ESTIMATION LETHAL DOSE OF SALMONELLA MBANDAKA INOCULATED EXPERIMENTALLY IN MICE.

Salmonella is an important food borne pathogen worldwide. This study was intended for in vivo to estimation lethal dose of Salmonella mbandaka isolated from human infantile diarrhea by calculating the lethal dose (LD50 ) , using mice (BALB/c) of both gender with age range from 6 to 8 weeks old, which drenched orally . The mice were monitored daily for a maximum of 30 day , the rested fourty two mice were divided randomly into seven groups each of its have 6 mice. The six groups of mice inoculated orally with one of the calculated (CFU/ml) diluents by using polyethylene tubes about (0.5) ml and the seven group inoculated Phosphate Buffer Saline (pH=7.2) and considered as a control group. The lethal dose (LD50) of S. mbandaka in mice was (1.3 ×109.5 cells / ml ).