ANTI-HYPERGLYCEMIC AND IN-VITRO ANTIOXIDANT EFFECT OF KIGELIA PINNATA FRUIT.

Diabetes mellitus (DM) is a complex metabolic disease characterized by chronic hyperglycaemia commonly associated with oxidative stress. The present study aims at evaluating the antioxidant and anti-hyperglycemic potential of various concentrations of hydroalcoholic extract of Kigelia pinnata fruit. The in-vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH), nitrDiabetes mellitus (DM) is a complex metabolic disease characterized by chronic hyperglycaemia commonly associated with oxidative stress. The present study aims at evaluating the antioxidant and anti-hyperglycemic potential of various concentrations of hydroalcoholic extract of Kigelia pinnata fruit. The in-vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH), nitric oxide, hydroxyl ion and hydrogen peroxide for free radical scavenging properties of the extracts, and determining their phenol and flavanoid contents. The antidiabetic activity was tested in rats following streptozocin induced hyperglycemia. The in-vitro antioxidant activity using DPPH, nitric oxide radical, hydroxyl radical and H2O2 radical showed IC50 value 77.49±0.38, 91.07±0.62, 118.91±0.43 and 86.46±0.52 ?g/ml respectively. The hydroalcoholic extract of the fruits with dose 250, 500 and 750 mg/Kg exhibited anti-hyperglycemic activities with 37.08%, 55.08% and 56.19% reduction, respectively. These findings suggest that K. pinnata fruits possess slight antidiabetic and good antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules against oxidative stress or it associated disorders.ic oxide, hydroxyl ion and hydrogen peroxide for free radical scavenging properties of the extracts, and determining their phenol and flavanoid contents. The antidiabetic activity was tested in rats following streptozocin induced hyperglycemia. The in-vitro antioxidant activity using DPPH, nitric oxide radical, hydroxyl radical and H2O2 radical showed IC50 value 77.49±0.38, 91.07±0.62, 118.91±0.43 and 86.46±0.52 ?g/ml respectively. The hydroalcoholic extract of the fruits with dose 250, 500 and 750 mg/Kg exhibited anti-hyperglycemic activities with 37.08%, 55.08% and 56.19% reduction, respectively. These findings suggest that K. pinnata fruits possess slight antidiabetic and good antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules against oxidative stress or it associated disorders.