EFFECT OF CURCUMIN PRETREATMENT ON CIPROFLOXACIN BIOAVAILBILITY IN RATS.

Aim:- The experiment was carried out to study the influence of curcumin pre-treatment on pharmacokinetic disposition of ciprofloxacin following single oral administration in adult male wistar rats. Method:-Twelve rats were divided into two groups of six each .Animals in group-I were administered ciprofloxacin (10mg/kg body weight p.o), while animals in group-II received similar dose of ciprofloxacin after pre-treatment with curcumin (400 mg/kg body weight p.o). Rats were anaesthetized after ciprofloxacin administration by xylazine (10-20 mg.kg-1 IP) and ketamine (44-100 mg.kg-1 IP). Blood samples (200 ?l) were collected into heparinised centrifuge tubes by tail vein clipping or jugular vein puncture as per convenience, prior to and at 0.33, 0.67,1, 1.5, 2, 4, 6, 8 and 12 h time intervals. Plasma concentrations of ciprofloxacin were determined by microbiological assay. Pharmacokinetic Analysis:-Plasma concentration versus time data of ciprofloxacin obtained in control and in pretreated group was utilized for calculating various pharmacokinetic parameters (?, t1/2?, AUC0-?, AUMC0-?, Vd, ClB, and MRT) by non compartmental methods (Gibaldi and Perrier, 1982) and computer software (PK Solver Version 2.0, 2010 by Zhang Yang). Statistical analysis:- All data were expressed as Mean±SEM. Differences in pharmacokinetic data between groups were analysed for statistical significance using unpaired student’s‘t’ test with Welch’s correction using ‘Instat’ software Results:- Peak plasma drug concentration (Cmax), AUC0-?, elimination half life t½?, Mean residence time (MRT) were significantly (p<0.05) longer in curcumin pretreated group. There is significant decrease in Steady state volume of distribution Vdss andtotal body clearance ClB in curcumin pretreated rats than in ciprofloxacin alone group. Conclusion:- The present study concluded that pretreatment with curcumin enhanced oral bioavailability of ciprofloxacin in rats.