EFFECT OF 790-805NM DIODE LASER THERAPY ON MAST CELL IN CUTANEOUS WOUND HEALING IN MICE.

Background and objective: -The use of low level laser therapy (LLLT) has been increased now a dayto accelerate healing of soft tissue injuries because of some biostimulatory effects. The goal of this study was to investigate the effect of 790-805nm diode laser on the inflammatory effect of mast cells during wound healing in rodents. Materials and Methods: -A cut wound (1.5cm) wasmadeon the cheek of 40albino mice. 20 of them exposed to LLLT (360 J/cm 2) at 790-805 nm immediately post wounding procedure. The animals were scarified and the wound area was prepared and stained by toluidine blue. Results: - Mean mast cell count of 10.2 in the first day of control group while in laser group 8.4. The control and laser group showed gradual inclination in the mean value to be return to increase at the day 14 of experiment. There was significant difference (P< 0.05) in control group at the first day. While significant difference (P?0.05) was in the day 7. Pearson correlation showed significant correlation (P?0.01) between the control group at the day 1 and the laser group at the day 7. While there was significant correlation (P?0.05) between the control group at the day 14 and laser group at the day 1. Conclusions:- LLLT may induce an anti-inflammatory effect on wound healing process by its inhibitory action on mast cells; while it may have a biostimulatory effect on the proliferation of mast cells at proliferative phase of wound healing which indirectly affects fibrous tissue regeneration in subcutaneous area.