DETECTION OF MUTANT DNA REPAIR GENE (OGG1) POLYMORPHISM IN SOME IRAQI PATIENTS TYPE1 DIABETES MELLITUS.

Background: Hyperglycemia and free fatty acid consumption are among the reasons for formation the oxidative stress. It may not be unexpected that diabetic subjects tend to have extra oxidative cell and reactive oxygen species) ROS) generation than healthy subjects. So the accumulation of reactive oxygen species (ROS) may generate extra damage to various biological macromolecules, counting DNA. The study aimed to compare the genetic distribution of polymorphism genotype (OGG1) in Type1 diabetes with a number of healthy people. Methods: The study involved a total (60) subjects including (30) people are suffering from diabetes Type1and (30) non-diabetic controls. All samples were taken from the Diabetes Center of the University of Al-Mustansiriya. Results: The result showed all samples (patients and controls) bands when analyzed by the wizard genomic DNA purification Kit (Intron, Korea), also the result revealed that the amplified DNA products band of OGG1 gene at level 207 bp in controls and in some patients. Conclusion: our studies showed the OGG1 gene polymorphism appeared intype1 diabetes which may lead to many complications in diabetes patients that would be appeared in the future.