MULTIDRUG-RESISTANT ACINETOBACTERBAUMANNII IN ADJARA REGION.

Background:- Carbapenem resistance in A. baumannii is now an emerging issue worldwide. Hospital outbreaks have been described from various geographic areas and this organism has become endemic in some of them. Emergence and spread of multidrug-resistant (MDR) Acinetobacter baumannii in nosocomial settings has become a serious concern in clinical practice. Acinetobacter baumannii has multidrug-resistant phenotypes. Resistance to broad spectrum ?-lactams, aminoglycosides, fluoroquinolones, and carbapenems are observed in this bacteria, which complicate the treatment of this pathogen Objectives: The current study aimed to identify resistance isolate Acinetobacter baumannii from different wards of a teaching hospital in Georgia, Adjara and determine the susceptibility pattern of these bacteria. Materials and Methods:- Susceptibility profile and identification of the infection Acinetobacter baumannii (n=14) isolates collected in different hospital services (2013-2015) were performed by disc diffusion methods according to the CLSI guidelines, and API 20E, respectively. Disk diffusion method was employed to evaluate antimicrobial susceptibility against CTX-cefotaxime, PIP-Piperacilin, DOR-Doripenem, TIC-Ticarcilin, MRP-Meropenem, FEP-Cefepim, CAZ-Ceftazodom, IMI-Imipenem, TIC/ACC- Ticarcilin/Clavulonic, CIP-ciprofloxacin, STX-Sulfonamid+timetri, CN-Gentamicin, TE-Tetraciclin, TOB-Tobramicin, PIP/TAZ-Piperacilintazobactam, NET-Netilmicin, CT-Colistin and AK-amikacin. Genes of family’s blaOXA51, blaOXA40, blaOXA58 and blaOXA23 group were investigated by PCR. Sequencing was performed using group-specific primers Results:- Among the 14 isolated A. baumannii, Samples cultured from the Sputum (25%), Biological fluid (17%),.Most of the isolates (60%) were obtained from intensive care unit (ICU). Isolated A. baumannii showed high resistance to the evaluated antibiotics except TE-Tetraciclin, which showed only 21.4 % resistance. Also, 78.57% and 100% of the isolates were identified as MDR. Conclusions:- The result of the current study showed the growing number of nosocomial infections associated with XDR A. baumannii causing difficulties in antibiotic therapy. Resistant strains increasingly cause public health problems; therefore, their early detection is essential for healthcare centers.