The study of the Toxic Effects of Ochratoxin A the purified from fungus Aspergillus niger in vivo of White rats and Evaluating the Ability of the Bio-lotion Floramil in the Reduction of Its Toxicity.

The study has the selection of isolates of A.niger fungus and examining their ability to produce the Ochratoxin A and defining the toxic effects on some of the anatomic properties of the males of white rat. Moreover assessing the capability of the bio-lotion Floraml in reducing its toxicity , and when testing the ability of isolates of the fungus that is examined to produce the Ochratoxin A by the use of the technology Thin layer Chromatography (TLC) shows that seven isolates producing this toxin out f 20 isolates and the most productive was the isolate A.niger16, also the technology (PCR) that the selected isolates (AN3,An6,An7,AN9,AN10,AN16, AN,19) A.niger DNA has contained the gene (PKS). The results of the histological study for the animal of the white rat that is treated with the Ochratoxin A where the tissue sections that have been taken from the liver shown the existence of pathological effects like a sever congestion in the tissues of the liver and loss of the radiative arrangement of the liver cells and an expansion in the liver pockets and breeding of the kupffer cells , while the kidney has been affected by the Ochratoxin A and the effect is represented in atrophy of the glomeruli and an expansion of the renal tubule twisted with ( Degredation) and alienation in the cells of lining the renal tubules twisted , and the intestine emerges in it a damage of the villi with a ( Desequemation)with infiltration of inflammatory cells specially (Macrophage),There was an a role for the bio-lotion of the bacteria Psedomonas fluorescens in protecting the organs of the males of the white rat that is treated with OchratoxinA with the bio lotion floramil from the toxic effects of the Ochratoxin A with the existence of slight changes of the tissue in the organs of the rats treated with it. As for the influence of the bio-lotion in the tissues of the liver , kidney and the intestine, the results of test have shown and the laboratory diagnosis for the tissue sections for the mentioned organs that there is no occurrence of change of pathological tissue in the treatment of the bio-lotion floramil only and it is the symmetry of the tissue sections of the comparison treatment that referred to no pathological changes.