In Vitro Morphogenesis of Stevia rebaudiana, A naturally occurring sweet plant

Stevia rebaudiana is likely to become as a major source of high potency sweetener for the growing natural food market worldwide. The present investigation was conducted to develop a fast and efficient protocol to propagate S. rebaudiana by tissue culture. Murashige and Skoog (MS) basal supplemented with 6-Benzylamino purine (BAP) at 13.5 µM and ?-Napthaleneacetic acid (NAA) at 8.0 µM showed best performance as compared to (BAP) alone at 13.3 µM for bud breaking. While two different combinations of BAP at 13.3 µM with Adenine sulphate (ADS) at 61.8 µM and BAP at 13.5 µM with (NAA) at 8.0 µM in MS basal medium resulted in maximum shoot regeneration and development. The combination of (NAA) at 10.7 µM with (BAP) at 8.8 µM resulted in the highest percentage (100%) of callus initiation. The best rooting was observed with (NAA) at 16.1 µM with (BAP) at 10.7 µM than Indole Acetic Acid (IAA) at 16.1 µM alone.