30Nov 2016


  • Department of biotechnology/College of Science/Baghdad University/Iraq.
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This study was designed to search for HLA-G polymorphisms and its relationships with unexplained recurrent spontaneous abortion (URSA) among Iraqi women. In this study 300 aborted women have been chosen and they had history of recurrent spontaneous abortion as case group; in all cases full history and complete examinations including body weight, height and body mass index were done. Furthermore, all of the patients were screened for various known causes of miscarriages, including Anticardiolipin antibodies (IgM) , antiphospholipid (IgM), Coagulation factors including protein C, protein S, Antithrombin III, activated protein C resistance(APCR), and investigation of toxoplasmosis antibodies (IgM) and cytomegalovirus antibody (IgM), rubella antibody (IgM), as well homocysteine level , TSH and progesterone hormones. Based on the results of the screening test 33.6 % of cases are of unknown cause, So out of 300, only 100 case were enrolled in this study to investigate the association between RSA and three HLA-G alleles ( HLA-G*0103, HLA-G*0104 and HLA-G*0105N) and 14-bp insertion/deletion polymorphism in exon 8 of the 3untranslated region (3 UTR)frequency and genotypes in idiopathic RSA in Iraqi women compared with 100 control women without previous history of RSA, with at least two successful pregnancies In addition, the ELISA was conducted to investigate HLA-G serum levels in women with URSA and healthy women. The frequency of the allele and genotypes in the patient group and control group was determined using PCR-restriction fragment length polymorphism (PCR-RFLEP). Exon 3 of HLA-G gene amplified using specific primers then digested by PpuM-I and BseR-I restriction enzymes, in order to detect HLA-G*0105N and HLA-G*0104 alleles respectively. While exon 2 of HLA-G gene amplified using specific primers then digested with HinfI restriction enzyme to detect HLA-G*0103 allele. Genotypes for the mentioned alleles determined and tested for their association with URSA patients. Results of HLA-G allele frequency showed significant association with increased risk of URSA patients. HLA-G*0105N and HLA-G*0104 allele was present in URSA patients and fertile controls. HLA-G*0105N allele was found in 48.7% of the patient group while, it was present in 51.3% of the control group, odds ratio was 11.5759. HLA-G*0104 allele was present with high significant frequency of 71.3% in the patient group and 28.7% in the controls, odds ratio was 4.8947. While HLA-G*0103 allele was not encountered neither in the patient group nor in the control group. Aforementioned alleles genotyped, and two genotypes were encountered in both URSA patients and fertile control groups (HLA-G*0105N/0105N and G*0104/0104) whereas the third genotype (HLA-G*0104/0105N) was absent in the control group. HLA-G*0104/0105N genotype was interesting as it was evident in 10 patients (100%) whereas none of the fertile controls showed this genotype. Other genotypes presented in both URSA patients and fertile controls. HLA-G*0105N/0105N genotype showed the frequency (12%) in patient groups and (13%) in control groups with odds ratio (0.9126) in both groups, (30%) of the patients revealed in HLA-G*0104/ 0104 genotype whereas (27%) of the control group harbored this genotype with odds ratio 1.1587.

[Reema M. Abd, Hasan F Al-Azzawie and Kareem Al-Kazzaz. (2016); GENETIC POLYMORPHISMS OF HLA-G ANTIGEN IN IRAQI WOMEN WITH UNEXPLAINED RECURRENT MISCARRIAGE. Int. J. of Adv. Res. 4 (Nov). 1654-1663] (ISSN 2320-5407). www.journalijar.com

hasan fayadh samir


Article DOI: 10.21474/IJAR01/2258      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2258