MICROBIOLOGICAL AND MOLECULAR CHARACTERIZATION OF SULPHUR OXIDIZING PSEUDOMONAS SP.PRK786 ISOLATED FROM CATTLE MANURE COMPOST

Rural based cattle manure compost inhabitant bacteria (sulphuroxidizing- Pseudomonas sp.PRK786) were characterized. The microbial physiologyaspect shows:chloramphenicolsensitive, gram-negative, non-motile, non-endospore forming, and transparent yellow color colonies were obtained. Besides the following biochemical characteristics such as:nitrate reducing,glucose dependent and non-pyocyanin pigments producing colonies were shown in thiosulphate agar medium. Moreover, it is significantly reducing the pH of the thiosulphate broth from 8.0 to 1.5.The sulfur oxidizing behavior of Pseudomonas sp.PRK786 was determined by sulphate ions accumulation in culture supernatant. Results, ~60% of sulphates are precipitated (without the formation of sulfur globules). Molecular aspects: It carries the ~4.5Mb size genomic DNA (A260/A280 ratio -1.52), 1285bp 16S rRNA gene (16S rRNA-PCR amplified product size was 1500bp), the 16S rRNA gene sequences showed 100% similarity with Pseudomonas sp.database, the free energy of consensus sequence of 16S rRNA was -396.80 kcal/mol and EMBL-EBI - accession number is HG931346.