Molecular Detection of Toxoplasma gondii in Human and Chicken by Real-Time PCR Technique

The Toxoplasma gondii is one of the most common zoonotic infectious pathogenic parasites and can be infected the human and animals. Regarding to transmission of disease by feline, the chicken can be a risk factors for Toxoplasma infection by transmission the oocyct. Current diagnosis of toxoplasmosis dependent on serologicaldetectionit may fail todetect specific anti-Toxoplasma IgG orIgM during the active phase of T. gondii infection, becausethese antibodies may not be produced until after several weeksof parasitemia. Therefore, in this study we used highly specific molecular as Real-Time PCR based TaqMan probe and primers to amplify the T. gondii B1 gene for detection of Toxoplasma gondii. Our results recorded the detection of Toxoplasma gondii in positive blood samples by LAT test collected from aborts woman and local breed chicken at (16%) and (24%) respectively. We conclude that real-time PCR a rapid, sensitive molecular technique specific for detection of T. gondii in human, animals and the chicken may be play important role in transmission of disease. Using the Real-Time PCR technique as detection tools of toxoplasma gondii in human and chicken was reported at first time in Iraq.