Vol. 5 (04) pp. 471-474 DOI: 10.21474/IJAR01/3834

OPTIMIZATION OF BACTERIAL INOCULUM CONCENTRATION AND pH FOR THE PRODUCTION OF AMYLASE AND PROTEASE USING Proteus sp. FROM SAGO EFFLUENT.

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  • II-M.Sc. Biochemistry, Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamilnadu, India.
  • Assistant Professor, Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamilnadu, India.
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Abstract

Sago is the edible starch in the form of globules extracted from tubers of Manihotesculenta commonly known as Tapioca/Cassava plant. Sago effluent contains large amount of organic material which affect the fertility of soil in agricultural lands. The aim of this study was to isolate and identify amylase and protease producing bacterial strain from sago factory effluent and it is identified as Proteus sp.Optimization of culture conditions such as various concentration of bacterial inoculum (0.5%, 1.0%, 1.5% and 2.0%) and various range of pH (6, 7, 8, 9) was performed at different time interval (24hrs, 48hrs and 72hrs) for the maximum production of amylase and protease using Proteus sp. isolated from sago effluent. Bacterial inoculum concentration of 1.0% and 2.0% shows 100?g/ml of amylase production, 420?g/ml of protease was produced by 0.5%, 1.0% and 1.5% bacterial inoculum concentration and the bacterial inoculum concentration of 0.5% and 1.0% shows 8000?g/ml of protein production at 72 hours incubation period. Optimum pH for the production of amylase and protease was found to be 9. Amylase and protease production at pH 9 was found to be 126?g/ml and 690?g/ml. Protein production at pH 9 was found to be 1200 ?g/ml.

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References

  1. Anbukumar,S., Narad Muni Prasad, Ashok Kumar,M.(2014).Effluent treatment for sago industry using Zeolie and activated carbon.Open Journal of Water pollution and Treatment.
  2. Dabananda S.N and Kshetri P.(2010).A thermostable alkaline protease from a moderately halo-alkalithermotolerant Bacillus subtilis strain SH1. J. Basic Appl. Sci.10: 5126-5134.
  3. Lowry O.H, Rosebrough N.J, Farr A.L and Randall R.J.(1951) Protein measurement with the Folin phenol reagent. Biochem.,193: 265-275.
  4. MacFaddin J.F. (1980) Biochemical tests for identification of medical Bacteria, 2nd edition, Williams, Baltimore., 1-2.
  5. Miller G.L.(1959).Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem.,31: 426-428.
  6. Oyeleke,S.B., and Oduwole,A.A.(2009). Production of amylase by bacteria isolated from a cassava dumpsite in minna, Nigerstate, Nigeria. African Journal of Microbiology Research, 3(4): 143-146.
  7. Pandey,A.,Nigam,P.,Soccol,C.R.,Socool,V.T.,Singh,D.&Mohan,R.(2000).Advances in microbial amylases.Biotechnol.Appl.Biochem.,31:13-12.
  8. Rai,S.K., Roy,J.K., and Mukherjee,A.K.(2010).Characterization of a detergent stable alkaline protease from a novel thermophilic strain Paenibacillus tezpurensis sp. Nov. AS-S24-II. Micribiol. Biotechnol. 85: 1437-1450.
  9. Singh J, Vohra R and Sahoo D.(1999).Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus. Lett.,21: 921-924.
  10. Synowiecki,J.,(2010).Some applications of thermophiles and their enzymes for protein processing. Afr. J. Biotechnology, 9: 7020-7025.
  11. Tambekar D.H, Kalikar M.V, Shinde R.S, Vanjari L.B and Pawar R.G.(2009).Isolation and characterization of multiple enzyme producer Bacillus species from saline belt of purna river. Appl. Sci. Res.5: 1064-1066.
  12. Windish W.W and Mhatre N.S. (1965).Microbial amylases. In: Wayne WU editor. Advances in applied microbiology. New York: Academic Press. 7: 273-304.

How to Cite This Article

S. Kavipriya and P. Kousalya. (2017); OPTIMIZATION OF BACTERIAL INOCULUM CONCENTRATION AND pH FOR THE PRODUCTION OF AMYLASE AND PROTEASE USING Proteus sp. FROM SAGO EFFLUENT., Int. J. of Adv. Res., 5 (04), 471-474, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/3834

Corresponding Author

S.KAVIPRIYA
II-M.Sc. Biochemistry, Department of Biochemistry, Bharathidasan College Of Arts and Science, Erode, Tamilnadu, India.
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.