20Jan 2017

STRUCTURAL VALIDATION AND HOMOLOGY MODELING OF DIFFERENTIALLY EXPRESSED PROTEINS IN RATTUS NORVERGICUS INDUCED BY BISPHENOL A AND PROBIOTIC TREATEMENT

  • Department of Microbiology, S.V. University, Tirupati-517 502, Andhra Pradesh, India.
  • Assistant Professor, Department of Microbiology, S.V.University, Tirupati-517 502 , Andhra Pradesh, India.
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The aim of the present study was to apply Bio informatics tools to the proteins expressed in alteration with Bisphenol A. The structure-based computational methods are needed to help, identify and characterize protein-protein complexes and their function. Differentially expressed Proteins were Tryptic digested and were analyzed by MALDI-TOF to identify peptide masses afterward used for MS/MS. For individual proteins, the most successful technique is homology modeling. Based on their mass to charge ratio, the expressed proteins sequences were collected from Mascot search data. The sequences were analysed with the help of Phyre-2 server, RasMol version 2.6 software, BLAST, QMEAN servers, SWISS-PROT, QMEAN servers, STRING and Rampage validation tool.

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[S. Preethi and J. Pramoda Kumari. (2017); STRUCTURAL VALIDATION AND HOMOLOGY MODELING OF DIFFERENTIALLY EXPRESSED PROTEINS IN RATTUS NORVERGICUS INDUCED BY BISPHENOL A AND PROBIOTIC TREATEMENT Int. J. of Adv. Res. 5 (Jan). 1134-1151] (ISSN 2320-5407). www.journalijar.com

Dr.J.PRAMODA KUMARI
Assistant Professor, Depaertment of Microbiology, Sri Venkateswara University, Tirupati-517 502,Andhra Pradesh, India.

DOI:

Article DOI: 10.21474/IJAR01/2863      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2863

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