HISTOLOGICAL EVALUATION OF RESPONSE TO DIRECT PULP CAPPING WITH PROPOLIS: EXPERIMENTAL STUDY IN RABBIT.
- Dentist in Faculty of Dentistry Hospital.
- Professor of Pediatric and Community Dentistry and Vice Dean for education and student Affairs, Faculty of Dentistry, Minia University.
- Lecturer of Pediatric and Community Dentistry, Faculty' of Dentistry', Minia University.
- Lecturer of Oral Pathology, Faculty of Dentistry, Minia University.
- Abstract
- Keywords
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Herbals have been used for centuries to prevent and control disease. Herbal extracts are effective because they interact with specific chemical receptors within the body and are in a pharmacodynamic sense than drugs themselves so usage of herbal extract averte many patients from many side effects that generally come with traditional medicines. Propolis gained a popularity in the field of Dentistry because of its antimicrobial, anti-inflammatory, healing, anesthetic and cariostatic properties. The aim of the current study was to evaluate the histological response of a healthy rabbit pulp to direct pulp capping with propolis compared to calcium hydroxide. Twelve male rabbits were selected, their dental pulps were intentionally exposed by using low speed round bur on labial surfaces of permanent central incisors. Split mouth technique was used for applying the capping material to control and experimental groups. Each group was subdivided into three subgroups, four rabbits for each, where rabbits were sacrificed after 1, 2 and 4 weeks from capping time respectively. Teeth were dissected after animal scarification and prepared for histopathologic and histochemical evaluation using Hematoxylin_ Eosin (HE) and Trichrome stains. The results showed that 25%, 50% and 25% of dental pulps capped with calcium hydroxide showed mild, moderate and severe inflammatory response respectively, while in propolis group 75% and 25% showed slight to moderate inflammatory response respectively. As regard hard tissue formation in response to capping materials, in calcium hydroxide group half of the cases showed moderate deposition and other half showed marked hard tissue deposited at fourth week of follow up period. While in propolis group there was a marked deposition in 75% of cases and moderate deposition in 25% in the other. Conclusion propolis proved to have less intense inflammatory response and better quality dentin bridge formation.
- Accornite ML, Loguerico AD, Reis A, Muench A, Araujo VC (2005): Response of human pulps capped with bonding agent after bleeding control with menostatic agents. Oper Dent;30: 147?155.
- Ahangari Z, Naseri M, Jalili M, Mansouri Y, Mashhadiabbas F, Torkaman A (2012): Effect of propolis on dentin regeneration and the potential role of dental pulp stem cell in Guinea pigs. Cell J.;13(4):223-228.
- Ansorge S, Reinhold D, Lendeckel U (2003): propolis and some of its constituents down-regulate DNA synthesis and inflammatory cytokine production but induce TGF-beta production of human immune cells. Z Naturforsch C. Jul-Aug; 58 (7-8) : 580- 589.
- Belduz N, Yilmmaz Y, Ozbek E, Kalkan Y, Demirci T (2010). The effect of neodymium-doped yttrium aluminum garnet laser irradiation on rabbit dental pulp tissue. Photomed Laser Surg. Dec; 28 (6): 747-750.
- Briso ALF, Rahal V, Mestrener SR, Dezan E Jr (2006): Biological response of pulps submitted to different capping materials. Braz Oral Res.; 20(3):219-225.
- Costa CA, Giro EM, do Nascimento AB, Teixeira HM, Hebling J (2003): Short-term evaluation of the pulpo-dentin complex response to a resin-modified glass-ionomer cement and a bonding agent applied in deep cavities. Dent Mater. Dec;19(8):739-746.
- Esmeraldo MR, Carvalho MG, Carvalho RA, Lime Rdef, CostaEM (2013): Inflammatory effect of green propolis on dental pulp in rats. Braz Oral Res. Sep- Oct: 27 (5) 417_422.
- Estrela C, Pimenta FC, Ito IY, Bammann LL (1999): Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules. J Endod, Jun; 25(6):416-418.
- Fenwick N, Danielson P, Griffin G (2011): Survey of Canadian animal-based researchers' views on the Three Rs: replacement, reduction and refinement. PLoS One.; 6(8).
- Fridland M, Rosado R (2005): MTA solubility: a long term study. J Endod. May;31(5):376-379.
- Haddad M, Lefranc G, Aftimos G (2003): Local application of IGF1 on dental pulp mechanically exposed; in vivo study on rabbit. Bull Group Int Rech Sci Stomatol Odontol. Jan-Apr; 45(1): 12-17.
- Khayyal MT, El-Ghazaly MA, El-Khatib AS (1993): Mechanism involved in the anti-inflammatory effect of propolis extracts. Drugs Exp Clin Res; 19: 197-203.
- Konigswald Wv, Golenishev FN (1979): A method for determining growth rates in continuously growing teeth. Journal of Mammalogoy; 60: 397-400.
- Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Uskudar Guclu A, Akca E, Ozkok Tuylu A, Sorkun K, Tanyuksel M, Salih B. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007 Jun-Aug;13(3-4):140-145.
- Luketić S, Malcić A, Jukić S, Anić I, Segović S, Kalenić S (2008): Coronal microleakage of two root-end filling materials using a polymicrobial marker. J Endod.;34(2):201-203.
- Mustafa M, Saujanya KP, Jain D, Sajjanshetty S, Arun A, Uppin l, Kadri M (2012): Role of Calcium Hydroxide in Endodontics. Global Journal of Medicine and Public Health. 1(1):66-70.
- Nascimento AB, FontanaUF, Teixeira HM, Costa CA (2000): Biocompatibility of a resin-modified glass-ionomer cement applied as pulp capping in human teeth. Am J Dent. Feb; 13 (1) : 28-34.
- Ozorio JE, Carvalho LF, de Oliveria DA, de Sousa- Neto MD, Perez D (2012): Standardized propolis extract and calcium hydroxide as pulpotomy agents in primary pig teeth. J Dent Child (Chic). May- Aug; 79 (2): 53-58.
- Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL (1998): Antimicrobial activity of propolis on oral microorganisms. Curr Microbiol. Jan;36(1):24-28.
- Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, Thomas M (2010): A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal. Aust Dent J. Mar; 55(1):59-64
- Passcoe S, Gatehouse D (1986): The use of a simple haematoxylin and eosin staining procedure to demonstrate micronuclei within rodent bone marrow. Mutal Res. Aug; 164 (4): 273-243.
- Sabir A, Abbu CR, Agustiono P, Sosroseno W (2005): Hisological analysis of rat dental pulp tissue capped with propolis. J Oral Sci. Sep; 47 (3): 135-138.
- Scheller S, Ilewicz L, Luciak M, Skrobidurska D, Stojko A, Matuga W (1978): biological properties and clinical application of propolis. Experimental observation on the influence of ethanol extract of propolis (EEP) on dental pulp regeneration. Arzneimittelforschung.; 28 (2): 289-291.
- Tziafas D, Smith AJ, Lesot H (2000): Designing new treatment strategies in vital pulp therapy. J Dent. Feb;28(2):77-92.
- Woehrlen E (1997):. Evaluation of techniques and materials used in pulpal therapy based on a review of the literature: part 1. J Am Dent Assoc.; 97: 1154-1158.
- Xu Y, Luo L, Chen B, Fu Y (2009): Recent development of chemical components in propolis. Frontiers of Biology in China.;4(4):385?391.
[Asmaa M Gamal, Nagwa M Khattab, Tamer A Fouda and Safaa M Tohamy. (2017); HISTOLOGICAL EVALUATION OF RESPONSE TO DIRECT PULP CAPPING WITH PROPOLIS: EXPERIMENTAL STUDY IN RABBIT. Int. J. of Adv. Res. 5 (Feb). 2325-2333] (ISSN 2320-5407). www.journalijar.com
Lecturer of Oral Pathology, Faculty of Dentistry, Minia University