Molecular ecological typing of environmental isolates of Aspergillusterreus collected from desert regions in Iraq

Most environmental isolates ofA.terreus reveal phenotypic variation in colonies characters included colony color ,texture and reverse pigments in order to determine if these variations occurred due to environmental response or genetic variation,100 isolates of A.terreus were collected from arid region in Iraq in the period 2011- 2012.Ribotyping of ITS region by two universal primer ITS1 -ITS2,ITS1 -ITS4 and specific primer for A.terreus. Genotyping of 19 isolates by RAPD -PCR using oligonucleotide primer R108 (5´-GTATTGCCCT-3´) Phylogenetic tree dendrogram (UPGMA)were constructed by using UVI band software, and evaluated the similarity coefficient factor among 19 isolates of A.terreus. The results showed that the morphological characterization had less taxonomic value comparing with molecular characterization which showed powerful keys in the identification of A.terreus isolates. The Phylogenetic analysis by RAPD-PCR had been show provide taxonomically useful information created many ecological genotypes , R108 Primer generated a banding patterns composing of one to 5 bands in various sizes ranging from 100 to 1000 bp. Phylogenetic tree based on RAPD –PCR patterns showed variable similarity coefficient values.