31Jan 2017

EXTRACELLULAR L-ASPARGINASE PRODUCTION BY HALOTOLERANT STRAIN OF ENTEROBACTER HORMAECHEI ISOLATED FROM MARINE FISHES.

  • Department of Biotechnology, St. Peter’s College, Kolenchery, Kerala 682311, India.
  • School of Bioengineering, SRM University, Chennai, Tamil Nadu 603203, India.
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Bacterial isolates from marine fishes were screened for L-Asparginase production on M9 minimal agar medium. Eleven isolates were selected for the estimation of extracellular enzyme production by submerged fermentation. Highest enzyme activity observed was 39 IU/ml. Bacterial mass assessed as optical density using spectrophotometer showed strong correlation (p<0.01) with the extracellular enzyme production. In laboratory scale process optimization studies, conditions selected for L-asparaginase production were 400C temperature, 7.0 pH and 3% sodium chloride concentration. The yield has been increased to 42IU/ml upon fermentation with optimized process parameters. Taxonomic identity of the best extracellular enzyme producing bacterial isolate was studied by biochemical analysis, PCR amplification of a portion of 16S rDNA and comparative sequence analysis using NCBI tool. The isolate was identified as Enterobacter hormaechei. Halotolerance and sustained enzyme production at an elevated temperature might be advantages to exploit the bacterium on commercial scale for the production of extracellular L- asparaginase.

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[Sudha K., Rani John and Sivansankari T. R. (2017); EXTRACELLULAR L-ASPARGINASE PRODUCTION BY HALOTOLERANT STRAIN OF ENTEROBACTER HORMAECHEI ISOLATED FROM MARINE FISHES. Int. J. of Adv. Res. 5 (Jan). 2619-2625] (ISSN 2320-5407). www.journalijar.com

Sudha K.
Department of Biotechnology, St. Peter's College, Kolenchery, Ernakulam, Kerala 682311

DOI:

Article DOI: 10.21474/IJAR01/3052      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3052

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