30Mar 2017

ESTABLISHMENT OF REFERENCE INTERVAL FOR SOME BONE TURNOVER MARKERS IN HEALTHY, YOUNG, PREMENOPAUSAL EGYPTIAN FEMALES.

  • Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, El-Hadara, Alexandria, Egypt.
  • Department of Biostatistics, High Institute of Public Health, Alexandria University & FAMCO at UoD.
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Introduction :The appropriateness of Laboratory tests selection and reliability of reference interval is crucial for healthcare delivery and clinical diagnosis. Bone remodeling is a physiological process that occurs continuously in healthy adult bone tissue with coupling of both resorption and formation. Bone turnover markers (BTMs) are classified as either bone formation, or resorption markers. Several BTM reference intervals for healthy premenopausal women have previously been established. This study aims to establish robust reference intervals for some bone turnover markers namely ; alkaline phosphatase , Calcium . Phosphorous , 25(OH) vit D , Carboxy-terminal collagen cross links (CTX-I) in healthy premenopausal Egyptian females between the age of 30-45 yrs. Material and methods : The sample of this study was age-stratified random sample , one hundred fifty one females aged between 30-45years participated and only 131 fulfilled the inclusion criteria and completed all lab tests and the questionnaire. Results : The results showed no statistically significant correlation between age and any of the studied parameters except phosphorus (weak indirect correlation rs=-0.189) and Vit D (intermediate correlation rs=-0.58). Ca and phosphorous and ALK did not statistically differ from the manufacturer .Concerning CTX and Vit D the new averages were lower than that set by the manufacturer , the differences were statistically significant (t=-10.2, p=0.0001 for CTX, and t=-19.7, p=0.0001 for Vit D) . While, the new average for PTH was higher than that of the manufacturer, the difference was statistically significant (t=10.9, p=0.0001).Conclusion : Our trial for establishment of reference interval in Egyptian population may help in harmonization of reporting by Egyptian laboratories. The strength points of this study are the standardization of the preanalytical and analytical conditions with adherence to the CLSI guidelines recommendations together with considering seasonal variation. The small sample size and the absence of the bone mineral density data with relatively low level of 25OHD were the limitations of this study .For a new or improved assay for BTM it is important to include a larger sample size with for a clinical reliable reference range to be used in assessment of the efficancy of the antiresorptive therapy. Key words: Bone turnover markers, reference interval, osteoporosis, premenopause.

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[Rania Mohamed El Sharkawy, Noha S. Kandil and MoatazaM. Abdel Wahab (2017); ESTABLISHMENT OF REFERENCE INTERVAL FOR SOME BONE TURNOVER MARKERS IN HEALTHY, YOUNG, PREMENOPAUSAL EGYPTIAN FEMALES. Int. J. of Adv. Res. 5 (Mar). 1522-1531] (ISSN 2320-5407). www.journalijar.com

Rania Mohamed El Sharkawy
1Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, El-Hadara, Alexandria, Egypt

DOI:

Article DOI: 10.21474/IJAR01/3661      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3661

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