27Feb 2017

THE IMPROVEMENT OF BIOCOMPATIBILITY OF THE DENTAL TITANIUM ALLOY IMPLANTS WITH DLC COATING:IN VITRO AND IN VIVO STUDY.

  • Department of Oral Maxillofacial Surgery, Faculty of Medicine and pharmacy, Casablanca, Morocco.
  • Consultations and Dental Emergency Services, Prosthetic joint, Faculty of Dentistry, Casablanca. Hassan II University of Casablanca. Morocco.
  • Bacteriology and Cytogenetic Services, Pasteur Institute, Casablanca, Morocco.
  • Cell Culture Unit, Centre for Research and Training in Health Sciences, Faculty of Medicine and Pharmacy, Casablanca, Morocco.
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Titanium alloys (Ti6A14V) are biomaterials of choice in dentistry, due totheir resistance to the biological constraints of the environment. Their use still causes in a number of cases, problems of osseointegrationthat urge us to raise again the question of the interface of titanium alloys withmineralized tissues in particular. The purpose of this study isfirst to evaluate the biocompatibility of these implants in the biological medium while offering alternative proposals for improving the osseointegration of these implants. Two studies were first conducted for this evaluation: in vitro study on human fibroblast cell cultures and bacterial colonization in the presence of four bacterial strains (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Haemolytic streptococcus) with and without implants , in vivo study on Wistar wistarrats (n = 35) which consists in the implantation of the biomaterial at the gingival sulcus and the renal capsule ; procurement of target organs of the cytotoxicity (sulcus, kidney and liver) and their morphological study withan optical microscope. Our results showed that the implants of titanium alloy (Ti6A14V) indeed disrupt the cellular structure of the organs studied compared to controls. The proliferation of bacterial strains studied was substantially similar in culture plates with and without implants. The proliferation of human fibroblasts in cell culture showed no significant change between the implants pits and the control pits. We concluded that the implants of titanium alloy (Ti6A14V) were biocompatible in vitro but cause some problems in vivo. This could explain the negative impact of these implants on osseointegration. The surface of implantscouldbe a limiting factorfor the biocompatibility and the osseointegration of titanium alloys (Ti6A14V). We have then showed,with a similar histopathological study, that the coating of the implants of titanium alloywithDiamond- Like Carbon (DLC) reduce completely the observed effects in the kidney and the liver rat.

  1. Branemark R, Huaud J B, Bertrand M. L?Ost?oint?gration : un nouveau concept chirurgical dans la r?habilitation des amput?s. e-m?moires de l'Acad?mie Nationale de Chirurgie, 2006;5(4) : 36-40.
  2. A et al. Convergent methods assessing bone growth in an experimental model at dental implants in the minipig. Annals of Anatomy 196 ;(2014): 100?107
  3. P et coll. Histologic studies on osseointegration: Soft tissuesresponse to implant surfaces and components. A review. Dental Materials 27?; (2011)?:53?60
  4. Xu B, Zhang J, Brewer E, Tu Q, Yu L, Tang J, Krebsbach P, Wieland M, Chen J. Osterix Enhances BMSC-associated Osseointegration of Implants. J Dent Res, 2009; 88(11): 1003?7.
  5. Depprich R, Ommerborn M, Zipprich H, Naujoks Ch, Handsche J, Wiesmann H P, K?bler N R, Meyer U. Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces. Head& Face Medicine, 2008;4:29-31.
  6. Stadlinger B, Pilling E, Huhle M, Mai R, Bierbaum S, Scharnweber Det all. Evaluation of osseointegration of dental implants coated with collagen, chondroitin sulphate and BMP-4: an animal study Int J. Oral Maxillofac Surg, 2008; 37: 54?9.
  7. Chiesa R, Giavaresi G, Fini M, Sandrini E, Giordano C, Bianchi A, Giardino R. In vitro and in vivo performance of a novel surface treatment to enhance osseointegration of endosseous implants.Oral Surg Oral Med Oral Pathol Oral RadiolEndod,2007; 103:745-56.
  8. K et al. Cell/Material Interfaces: Influence of Surface Chemistry and Surface Topography on Cell Adhesion Journal of Adhesion Science and Technology 24 ;(2010): 831?852
  9. J.E et al. Topographic scale-range synergy at the functional bone/implant Interface. Biomaterials 35; (2014): 25-35.
  10. Faia-Torres.A.B et al. Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients. Biomaterials 35; (2014): 9023-9032
  11. N.M et coll. Mechanical properties and in vitro bioactivity of injectable and self-setting calcium sulfate/nano-HA/collagen bone graft substitute. Journal of the mechanical behaviour of biomedical materials 12; (2012): 119-128
  12. B et coll.Bone Tissue Engineering Around Dental Implants. Stem Cell Biology and Tissue Engineering in Dental Sciences; (2015): 749-764.
  13. C.H et al. Micropit surfaces designed for accelerating osteogenic differentiation of murine mesenchymal stem cells via enhancing focal adhesion and actin polymerization. Biomaterials 35; (2014): 2245-2252
  14. Kumari TV, Anil Kumar P R, Muraleedharan C V, Bhuvaneshwar G S, Sampeur Y, Derangere F, Suryanara R.In vitro cytocompatibility studies of Diamond Like Carbon coatings on titanium. Bio-Medical Materials and Engineering, 2002;12 : 329- 38.
  15. Nakayama T, et al. Polarization of osteoclasts on dental implant materials is similar to that observed on bone. J Oral Biosci (2014), http://dx.doi.org/10.1016/j.job.2014.06.005.
  16. Zhou J, Pavon-Djavid G, Anagnostou F, Migonney V. Inhibition de l?adh?rence de Porphyromonas gingivalis sur la surface de titane greff? de poly (styr?ne sulfonate de sodium).ITBM-RBM,2007; 28: 42?8.
  17. Gaucher H, Bentley K, Roy S, Head T, Blomfield J, Blondeau F et al. Une ?tude multicentrique sur les implants Osseotite comme support aux proth?ses mandibulaires: rapport apr?s 3 ans. J? Can Dent Assoc, 2001; 67(9):528-33.
  18. Comăneanu.R.M et al.Correlations between cyto-histopathological tissue changes at the dental implant interface and the degree of surface processing. Rom J Morphol Embryol 2014?; 55(2):335?34
  19. Prodanov.L et al. The effect of nanometric surface texture on bone contact to titanium implants in rabbit tibia. Biomaterials 34; (2013): 2920-2927
  20. Y et coll. A review of improved fixation methods for dental implants. Part I: surface optimization for rapid osseointegration. Journal of prosthodontic Research 59; 2015: 20-33
  21. E et al. Osteogenic differenciation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche.Osteogenesis of MSCs in a simplified bone niche. European Cells and Materials Vol.23?; 2012?:13-27.
  22. F, Mosca.C, Ramella.M, Valente.G & Cannas.M (2013) The effect of mechanical strain on soft (cardiovascular) and hard (bone) tissues, Cell Adhesion & Migration, 7:2, 165-173, DOI: 10.4161/cam.23020
  23. M et al .Calcium phosphate bone graft substitutes: Failures and hopes. Journal of the European Ceramic Society 32; (2012): 2663?2671
  24. Alanazi A, Nojiri C, Noguchi T, Kido T, Komatsu K K,Funakubo A. Improved blood compatibility of DLC coated polymeric materials. ASAIO J, 1999 ; 45.
  25. Alanazi A, Nojiri C, Kido T, Noguchi T, Ohgoe Y, Matsuda T et al. Engineering analysis of Diamond - Like Carbone caoted polymeric materials for biomedical applications. Artificial Organs,2000; 24(8): 624-627.

[Sidqui M, Slimani F, Marzak J, Hassar G, Bouzoubaa M, Chadly B, Elazhari M, Dersi N, Bourjilat FZ, Chekkoury El Idrissi A and Aboussaouira T. (2017); THE IMPROVEMENT OF BIOCOMPATIBILITY OF THE DENTAL TITANIUM ALLOY IMPLANTS WITH DLC COATING:IN VITRO AND IN VIVO STUDY. Int. J. of Adv. Res. 5 (Feb). 1490-1495] (ISSN 2320-5407). www.journalijar.com

Sidqui Mustapha
Consultations and Dental Emergency Services, Prosthetic joint, Faculty of Dentistry, Casablanca. Hassan II University of Casablanca. Morocco

DOI:

Article DOI: 10.21474/IJAR01/3305      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3305

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