NITRITE OXIDE DETECTION UTILIZING THE GRIESS ASSAY: ELUCIDATING INTERFERING FACTORS FOR IN VITRO APPLICATION.

Nitrite Oxide (NO) is a molecular mediator of many physiological processes including: Vasodilation, Inflammation, Immunity& Neurotransmission.But we can’t measure Nitrite Oxide formation Directly by Griess assay so we use one of its stable non-volatile breakdown products: the Nitrite ( NO2-).In our paper we look into different factors that may change the Nitrite Oxide Quantification in Griess Assay reaction for in-vitro application including:Culture Media(DMEM , RPMI , MEM and RPMI-F12) &Anticoagulants(Heparin And Enoxaparin). This is done by comparing the results from a control Griess Assay against a Griess Assay with either Culture media or Anticoagulant. From the results we conclude that a) Culture Media produce similar results to Calibration curve. B) Griess assay is effected by Heparin and Enoxoparin.