PROBIOTIC NON LACTIC ACID BACTERIA: STUDY OF BACTERIOCIN FOR THE ANTAGONISTIC ACTIVITY.

Bacteriocins are very useful as therapeutic agents. They act as an important and safer components as preservative and hence find their application in a wide range of medicine, agriculture, food and dairy products. Isolation of bacteriocins and its significant application has remained a challenging task. Production of bacteriocins of beneficial grade and their utilization as antibiotics and in food grade products without any harmful side effects also appear to be a tedious work. This work was initiated by isolation of non lactic acid bacteria from various food materials like pulses and cow?s milk, morphological identification by colony characteristics and microscopic examination by Gram?s staining. Qualitative identification of non lactic acid bacteria by catalase test. Bacteriocins production was detected by antimicrobial activity test against the indicator organisms by Kirby bauer disc diffusion method. Here we are presenting the results of the identification tests of the isolated bacteria and that of the antimicrobial studies. Future prospective includes the optimization of cultural, nutritional parameters and use of inducers to enhance the production, purification and characterization of bacteriocins. Significance of bacteriocins would be exploited in replacing the use of antibiotics and as preservative in food industries.