REMOTE ISCHEMIC PRECONDITIONING AND N-ACETYLCYSTEINE IN AUTOLOGOUS OVARIAN TRANSPLANTATION IN RATS.

  • Laboratory of Experimental Surgery, Department of Experimental Surgery, Pará State University, Belem, Para, Brazil.
  • Department of Contemporary Amazon Parasitology, Pará State University, Belém, Para, Brazil.
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Background: Ovarian cryopreservation is a relevant approach to fertility preservation, even though it is an invasive and still experimental procedure. Graft’s vulnerability for ischemia and reperfusion injury before the neovascularization is the most important factor to determinate the viability of transplanted tissue, therefore in order to reduce this injury, many techniques were described as remote ischemic pre-conditioning (R-IPC) and early administration of N-acetylcysteine (NAC). The aim of this study was to evaluate the effects of remote ischemic preconditioning and N-acetylcysteine on autologous ovarian transplant in rats. Materials and Methods: 25 female Rattus norvegicus (Wistar) were distributed into five experimental groups (n=5): (1) control (CG); (2) ovarian transplantation (GTx); (3) ovarian transplantation + R-IPC; (4) Ovarian transplantation + NAC and (5) Ovarian transplantation + R-IPC + NAC. On the ?15?^th post-operative day, blood was collected from inferior vena cava for estradiol and progesterone serum analysis and ovarian grafts were removed for histopathology. Results: Estradiol levels between groups showed no statistical difference, however treatment with R-IPC promoted higher progesterone levels. Ovarian graft morphology indicated that NAC treatment increased secondary follicles and promoted atretic follicles as observed in transplant group. R-IPC treatment reduced atretic follicles. Conclusions: NAC and R-IPC treatments showed protective effects regarding follicles preservation and ovarian function.

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[Elisa Maria Novaes Barros, Andrew Moraes Monteiro, Fernando Mateus Viegas Brandao, Rodrigo Paracampo Couteiro, Larissa Vasconcelos de Castro, Rubens Fernando Goncalves Ribeiro Junior, Tatiane Lamarao Graaf, and Patrícia Brazao Cohen. (2017); REMOTE ISCHEMIC PRECONDITIONING AND N-ACETYLCYSTEINE IN AUTOLOGOUS OVARIAN TRANSPLANTATION IN RATS. Int. J. of Adv. Res. 5 (Feb). 1823-1830] (ISSN 2320-5407). www.journalijar.com

Elisa Maria Novaes Barros

DOI:

Article DOI: 10.21474/IJAR01/3347      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3347

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