28Feb 2017

ROLE OF GROWTH REGULATORS IN MICROPROPAGATION OF WOODY PLANTS-A REVIEW

  • Department of Botany, Dayanand College, Hisar-125001, Haryana (India).
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The success of micropropagation technique depends upon the use of growth regulators in the culture medium. Growth regulators regulate the growth and developmental processes. These are the key factors in initiating the process of regeneration in tissue culture. In most of in vitro studies, explants do not respond well on culture media without growth regulators. An interactive balance of auxins and cytokinins controlled the in vitro growth and differentiation response in plant tissues. This review highlights on the role of growth regulators in micropropagation of woody plants.

  1. Al-Safadi, B. and Elias, R. (2011): Improvement of caper (Capparis spinosa) propagation using in vitro culture and gamma irradiation. Sci. Hortic. 127:290-297.
  2. Anuradha, M. and Pullaiah, T. (1999): In vitro seed culture and induction of enhanced axillary branching in Pterocarpus santalinus & Pterocarpus marsupium: A method for rapid multiplication. Phytomorphol. 49:157-163.
  3. Arockiasamy, S.; Ignacimuthu, S. and Melchias, G. (2000): Influence of growth regulators and explant type on in vitro shoot propagation and rooting of red sandalwood (Pterocarpus santalinus). Indian J. Exp. Bot. 38:1270-1273.
  4. Arya, S.; Kalia, R.K. and Arya, I.D. (2000): Induction of somatic embryogenesis in Pinus roxburghii Plant Cell Rep. 19:775-780.
  5. Asthana, P.; Jaiswal, V.S. and Jaiswal, U. (2011): Micropropagation of Sapindus trifoliatus and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiol. Plant. 33:1821-1829.
  6. Babu, K.N.; Sajina, A.; Minoo, D.; John, C.Z.; Mini, P.M.; Tushar, K.V.; Rema, J. and Ravindran, P.N. (2003): Micropropagation of camphor tree (Cinnamomum camphora). Plant Cell Tiss. Org. Cult. 74:179-183.
  7. Bala, R.; Beniwal, V.S. and Laura, J.S. (2015): An efficient and reproducible indirect shoot regeneration from female leaf explants of Simmondsia chinensis, a liquid-wax producing shrub. Physiol. Mol. Biol. Plants 21(2):293-299.
  8. Balaraju, K.; Agastian, P.; Ignacimuthu, S. and Park, K. (2011): A rapid in vitro propagation of red sanders (Pterocarpus santalinus) using shoot tip explants. Acta Physiol. Plant. 33:2501-2510.
  9. Balaraju, K.; Agastian, P.; Preetamraj, J.P.; Arokiyaraj, S. and Ignacimuthu, S. (2008): Micropropagation of Vitex agnus-castus, (Verbenaceae)-a valuable medicinal plant. In Vitro Cell. Dev. Biol.-Plant 44:436-441.
  10. Bosela, M.J. and Michler, C.H. (2008): Media effects on black walnut (Juglans nigra) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinins types. In Vitro Cell. Dev. Biol.-Plant 44:316-329.
  11. Buendia-Gonzalez, L.; Estrada-Zuniga, M.E.; Orozco-Villafuerte, J.; Cruz-Sosa, F. and Vernon-Carter, E.J. (2012): Somatic embryogenesis of the heavy metal accumulator Prosopis laevigata. Plant Cell Tiss. Org. Cult. 108:287-296.
  12. Buendia-Gonzalez, L.; Orozco-Villafuerte, J.; Cruz-Sosa, F.; Chavez-Avila, V.M. and Vernon-Carter, E.J. (2007): Clonal propagation of mesquite tree (Prosopis laevigata & Bonpl. ex Willd. M.C. Johnston). In Vitro Cell. Dev. Biol.-Plant 43:260-266.
  13. Bunn, E. (2005): Development of in vitro methods for ex situ conservation of Eucalyptus impensa, an endangered mallee from southwest Western Australia. Plant Cell Tissue Org. Cult. 83:97-102. DOI: 10.1007/s11240-005-3275-2.
  14. Chand, S. and Singh A.K. (2004): In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium In Vitro Cell. Dev. Biol.-Plant 40:464-466.
  15. Cheruvathur, M.K. and Thomas, T.D. (2011): An efficient plant regeneration system through callus for Pseudarthria viscida (L.) Wright and Arn., a rare ethnomedicinal herb. Physiol. Mol. Biol. Plants 17:395-401.
  16. Du, N. and Pijut, P.M. (2008): Regeneration of plantlets from Fraxinus pennsylvanica hypocotyls and cotyledons. Sci. Hortic. 118:74-79.
  17. Feng, J.C.; Yu, X.M.; Shang, X.L.; Li, J.D. and Wu, Y.X. (2010): Factors influencing efficiency of shoot regeneration in Ziziphus jujuba ‘Huizao’. Plant Cell Tiss. Org. Cult. 101:111-117.
  18. Gatti, E. (2008): Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance. Biol. Plant. 52:146-148.
  19. George, E.F. (1993): Plant propagation by tissue culture (part I: the technology). Exegetics LTD, UK.
  20. Ghimire, B.K.; Seong, E.S.; Nguyen, T.H.; Yu, C.Y.; Kim, S.H. and Chung, I.M. (2016): In vitro regeneration of Melastoma malabatricum through organogenesis and assessment of clonal and biochemical fidelity using RAPD and HPLC. Plant Cell Tiss. Organ Cult. 124: 417-529.
  21. Girijashankar, V. (2012): In vitro regeneration of Eucalyptus camaldulensis. Physiol. Mol. Biol. Plants 18:79-87.
  22. Gonzalez-Rodriguez, J.A.; Ramirez-Garduza, F.; Robert, M.L.; O’Connor-Sanchez, A. and Pena-Ramirez, Y.J. (2010): Adventitious shoot induction from adult tissues of the tropical timber tree yellow Ipe primavera (Tabebuia donnell-smithii rose [Bignoniaceae]). In Vitro Cell. Dev. Biol.-Plant 46:411-421.
  23. Gour, V.S. and Kant, T. (2009): In vitro regeneration in Emblica officinalis from juvenile root-derived callus. J. Indian Bot. Soc. 89:34-36.
  24. Gururaj, H.B.; Giridhar, P. and Ravishankar, G.A. (2007): Micropropagation of Tinospora cordifolia (Willd.) Miers ex Hook. F & Thoms- a multipurpose medicinal plant. Curr. Sci. 92:23-26.
  25. Husain, M.K.; Anis, M. and Shahzad, A. (2007): In vitro propagation of Indian Kino (Pterocarpus marsupium) using Thidiazuron. In Vitro Cell. Dev. Biol.-Plant 43:59-64.
  26. Inamdar, J.A.; Nataraj, M.; Mohan, J.S.S. and Subramanian, R.B. (1990): Somatic embryogenesis from callus cultures of Crataeva nurvala Ham. Phytomorphol. 40:319-322.
  27. Isogai, S.; Touno, K. and Shimomura, K. (2008): Gibberellic acid improved shoot multiplication in Cephaelis ipecacuanha. In Vitro Cell. Dev. Biol.-Plant 44:216-220.
  28. Joseph, N.; Siril, E.A. and Nair, G.M. (2011): An efficient in vitro propagation methodology for Annatto (Bixa orellana). Physiol. Mol. Biol. Plants 17:263-270.
  29. Karun, A.; Siril, E.A.; Radha, E. and Parthasarathy, V.A. (2004): Somatic embryogenesis and plantlet regeneration from leaf and inflorescence explants of areca nut (Areca catechu). Curr. Sci. 86:1623-1628.
  30. Krajnakova, J.; Haggman, H. and Gomory, D. (2009): Effect of sucrose concentration, polyethylene glycol and activated charcoal on maturation and regeneration of Abies cephalonica somatic embryos. Plant Cell Tiss. Org. Cult. 96:251-262.
  31. Kumar, A. (1992): Somatic embryogenesis and high frequency of plantlet regeneration in callus culture of Thevetia peruviana. Plant Cell Tiss. Org. Cult. 31:47-50.
  32. Kumar, A.; Ahmad, MD. S. and Naseem, MD. (2010): In vitro plant regeneration from organ culture of Gmelina arborea J. Indian Bot. Soc. 89:197-203.
  33. Kumar, A.D. and Seeni, S. (1998): Rapid clonal multiplication through in vitro axillary shoot proliferation of Aegle marmelos (L.) Corr., a medicinal tree. Plant Cell Rep. 17:422-426.
  34. Kumar, A.S. and Kumar, A. (1995): Plant regeneration from cultured embryonic axis in Thevetia peruviana Indian J. Exp. Biol. 33:190-193.
  35. Kumar, S.; Narula, A.; Sharma, M.P. and Srivastava, P.S. (2003): Effect of copper and zinc on growth, secondary metabolite content and micropropagation of Tinospora cordifolia: a medicinal plant. 53:79-91.
  36. Kumar, U.; Singh, I. and Vimala, Y. (2009): In vitro regeneration of Moringa oleifera. J. Indian Bot. Soc. 88:120-123.
  37. Kumari, N.; Jaiswal, U. and Jaiswal V.S. (1998): Induction of somatic embryogenesis and plant regeneration from leaf callus of Terminalia arjuna Curr. Sci. 75:1052-1055.
  38. Lee, Y.; Lee, D.E.; Lee, H.S.; Kim, S.K.; Lee, W.S.; Kim, S.H. and Kim, M.W. (2011): Influence of auxins, cytokinins and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree (Morus alba). Plant Cell Tiss. Org. Cult. 105:9-19.
  39. Marriott, P. and Sarasan, V. (2010): Noval micropropagation and weaning methods for the integrated conservation of a critically endangered tree species, Medusagyne oppositifolia. In Vitro Cell. Dev. Biol.-Plant 46:516-523.
  40. Mathur, G.; Arnold, S. V. and Nadgauda, R. (2000): Studies on somatic embryogenesis from immature zygotic embryos of chir pine (Pinus roxburghii ). Curr. Sci. 79:999-1004.
  41. Mathur, S.; Shekhawat, G.S. and Batra, A. (2002): Micropropagation of Salvadora persica via cotyledonary nodes. Indian J. Biotechnol. 1:197-200.
  42. Miller, C.O. and Skoog, F. (1953): Chemical control of bud formation in tobacco segments. Am. J. Bot. 40:768-773.
  43. Murashige, T. (1974): Plant propagation through tissue culture. A. Rev. Plant Physiol. 25:136-166.
  44. Nair, L.G. and Seeni, S. (2001): Rapid in vitro multiplication and restoration of Celastrus paniculatus sub sp. paniculatus (Celastraceae), a medicinal woody climber. Indian J. Exp. Biol. 39:697-704.
  45. Nayak, P.; Behera, P.R. and Manikkannan, T. (2007): High frequency plantlet regeneration from cotyledonary node cultures of Aegle marmelos (L.) Corr. In Vitro Cell. Dev. Biol.-Plant 43:231-236.
  46. Nayak, P.; Behera, P.R.; Thirunavoukkarasu, M. and Chand, P.K. (2010): High frequency plant regeneration through adventitious multiple shoot organogenesis in epicotyl explants of Indian gooseberry (Emblica officinalis Gaertn). Sci. Hortic. 123:473-478.
  47. Ndoye, M.; Diallo, I. and Dia, Y.K.G. (2003): In vitro multiplication of the semi arid forest tree, Balanites aegyptiaca (L.) Del. African J. Biotechnol. 2:421-424.
  48. Ortiz, B.O. C.; Reyes, M.E.P. and Balch, E.P.M. (2000): Somatic embryogenesis and plant regeneration in Acacia farnesiana and schaffneri. In Vitro Cell. Dev. Biol.-Plant 36:268-272.
  49. Palla, K.J. and Pijut, P.M. (2011): Regeneration of plants from Fraxinus americana hypocotyls and cotyledons. In Vitro Cell. Dev. Biol.-Plant 47:250-256.
  50. Peternel, S.; Gabrovsek, K.; Gogala, N. and Regvar, M. (2009): In vitro propagation of European aspen (Populus tremula) from axillary buds via organogenesis. Sci. Hortic. 120:109-112.
  51. Pinto, G.; Santos, C.; Neves, L. and Araújo, C. (2002): Somatic embryogenesis and plant regeneration in Eucalyptus globulus Plant Cell Rep. 21:208-213.
  52. Raghu, A.V.; Geetha, S.P.; Martin, G.; Balachandran, I. and Ravindran, P.N. (2006): In vitro propagation through mature nodes of Tinospora cordifolia (Willd.) Hook. F. & Thoms.: an important ayurvedic medicinal plant. In Vitro Cell. Dev. Biol.-Plant 42:584-588.
  53. Rai, M.K.; Akhtar, N. and Jaiswal, V.S. (2007): Somatic embryogenesis and plant regeneration in Psidium guajava cv. Banarasi local. Sci. Hort. 113:129-133.
  54. Ram, K. and Shekhawat, N.S. (2011): Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants. Physiol. Mol. Biol. Plants 17:281-289.
  55. Rout, G.R. (2005): In vitro somatic embryogenesis in callus cultures of Azadirachta indica Juss.-a multipurpose tree. J. For. Res. 10:263-267.
  56. Rout, G.R. and Nanda, R.M. (2005): Protocol of somatic embryogenesis in Acacia arabica (Lamk.) Willd. Forestry Sciences (Book Series). 77:401-411.
  57. Saklani, K.; Singh, S.; Purohit, V.K.; Prasad, P. and Nautiyal, A.R. (2015): In vitro propagation of Rudraksha (Elaeocarpus sphaericus) (Gaertn.) K. Schum): a biotechnolical approach for conservation. Physiol. Mol. Biol. Plants 21(4):611-615.
  58. Savita; Singh, B.; Virk, G.S. and Nagpal, A.K. (2011): An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Physiol. Mol. Biol. Plants 17:161-169.
  59. Shahzad, A. and Siddiqui, S.A. (2001): Micropropagation of Melia azedarach Phytomorphol. 51:151-154.
  60. Sharma, H. and Vashistha, B.D. (2010a): Effect of some auxins on regenerative potential of various explants of Cinnamomum camphora (L.) Nees & Eberm. Agri. Bio. Res. 15(1): 83-89.
  61. Sharma, H. and Vashistha, B.D. (2010b): In Vitro propagation of Cinnamomum camphora (L.) Nees & Eberm using Shoot Tip Explants. Biol. 26(2): 109-114.
  62. Sharma, H. and Vashistha, B.D. (2010c): Effect of some cytokinins on nodal explants of Cinnamomum camphora (L.) Nees & Eberm. Eco-Res. J. Biosci. 9(1&2): 81-86.
  63. Sharma, H. and Vashistha, B.D. (2011a): In vitro callus induction and plant regeneration in nodal explants of Tinospora cordifolia (Willd.) Miers. ex Hook. f. & Thoms. Agri. Bio. Res. 16(1): 7-11.
  64. Sharma, H. and Vashistha, B.D. (2011b): Effect of some plant growth regulators on regenerative potential of internodal explants of Tinospora cordifolia (Willd.) Miers. ex Hook. f. & Thoms. Biol. 27(1): 9-14.
  65. Sharma, H. and Vashistha, B.D. (2014): In vitro callus initiation and organogenesis from shoot tip explants of Tinospora cordifolia (Willd.) Miers ex Hook. f & Thoms. CIBTech J. Biotecnol. 3(4): 77-83.
  66. Sharma, H. and Vashistha, B.D. (2015a): Plant tissue culture: a biological tool for solving the problem of propagation of medicinally important woody plants- A review. J. Adv. Res. 3(2): 402-411.
  67. Sharma, H. and Vashistha, B.D. (2015b): Effect of some auxins on petiolar explants of Tinospora cordifolia (Willd.) Miers ex Hook. f & Thoms. Ann. Biol. 31(1): 28-31.
  68. Sharma, H. and Vashistha, B.D. (2015c): In vitro plant regeneration through callus in Giloy (Tinospora cordifolia (Willd.) Miers ex Hook. f & Thoms.). Ind. J. Sci. 12(34): 59-68.
  69. Sharma, H.; Vashistha, B.D.; Singh, N. and Kumar, R. (2015): Tinospora cordifolia (Willd.) Miers ex Hook. f & Thoms. (Menispermaceae): Rapid in vitro propagation through shoot tip explants. Int. J. Recent Sci. Res. 6(2): 2714-2718.
  70. Sharma, H. (2016): Cloning of economically important trees through micropropagation technique- A review. Global Res. Analysis. 4(2): 146-151.
  71. Shekhawat, M.S. and Manokari, M. (2016): In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis): a multipurpose treatened species. Physiol. Mol. Biol. Plants 22(1):131-142.
  72. Siwach, P. and Gill, A.R. (2011): Enhanced shoot multiplication in Ficus religiosa in the presence of adenine sulphate, glutamine and phloroglucinol. Physiol. Mol. Biol. Plants 17:271-280.
  73. Sujatha, K. and Hazra, S. (2007): Micropropagation of mature Pongamia pinnata In Vitro Cell. Dev. Biol.-Plant 43:608-613.
  74. Sun, L.; Hou, S.; Wu, D. and Zhang, Y. (2008): Rapid clonal propagation of Zygophyllum xanthoxylon (Bunge) Maxim., an endangered desert forage species. In Vitro Cell. Dev. Biol.-Plant 44:396-400.
  75. Tian, L.; Sibbald, S.; Subramanian, J. and Svircev, A. (2007): Characterization of Prunus domestica in vitro regeneration via hypocotyls. Sci. Hortic. 112:462-466.
  76. Tornero, O.P.; Lopez, J.M; Egea, J. and Burgos, L. (2000): Effect of basal medium and growth regulators on the in vitro propagation of apricot (Prunus armenica ) cv. Canino. J. Hort. Sci. Biotechnol. 75:283-286.
  77. Tyagi, P. and Kothari, S.L. (2001): Continuous shoot production for micropropagation of Capparis decidua- A tree of arid agroforestry system. J. Indian Bot. Soc. 8:5-8
  78. Tyagi, S. and Govil, C.M. (1999): Somatic embryogenesis and micro-propagation in Emblica officinalis J. Indian Bot. Soc. 78:363-365.
  79. Walia, N.; Sinha, S. and Babbar, S.B. (2003): Micropropagation of Crataeva nurvala. Biol. Plant. 46:181-185.
  80. Wang, B.; Peng, D.X.; Sun, Z.X.; Zhang, N. and Gao, S.M. (2008): In vitro plant regeneration from seedling-derived explants of ramie [Boehmeria nivea (L.) Gaud]. In Vitro Cell. Dev. Biol.-Plant 44:105-111.
  81. Zakavi, M.; Askari, H. and Irvani, N. (2016): Optimizing micropropagation of drought resistant Pyrus boissieriana Physiol. Mol. Biol. Plants 22(4): 583-593.

[Hemant Sharma. (2017); ROLE OF GROWTH REGULATORS IN MICROPROPAGATION OF WOODY PLANTS-A REVIEW Int. J. of Adv. Res. 5 (Feb). 2378-2385] (ISSN 2320-5407). www.journalijar.com

Dr. Hemant Sharma
Department of Botany, Dayanand College, Hisar-125001, Haryana (India)

DOI:

Article DOI: 10.21474/IJAR01/3421      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3421

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