PHYSIOLOGICAL, IMMUNOLOGICAL AND HISTOPATHOLOGICAL COMPARISON OF ECHINOCOCCUS GRANULOSUS (G6) CAMEL STRAIN BY DIFFERENT VIABILITY STATUS USING SECONDARY CYST DEVELOPMENT IN RAT

  • Department of Zoology, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt.
  • Parasitology Unit, Animal Health Department, Desert Research Center, Cairo, Egypt.
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The current study aimed to demonstrate the differences in hydatid cysts of Echinococcusgranulosus(G6) camel strain by different viability status using secondary cyst development in the rat and to study physiological, immunological and histopathological changes associated with.A comparative study was conducted along time of exposure in different groups of rats which intraperitoneally injected with three different status of the viability of hydatid cyst fluid of camel origin (G6).The groups were:low viability protoscolices (1), high viability protoscolices fluid (2), not viable and not completely transformed to the calcareous status of protoscolices fluid(3), and one negative control group. The immunobiology of host–E. granulosus interactions were evaluated by measuring IL-10 a cytokine associated with immunoregulation of effector responses. Histopathological changes were conducted in liver, lung,and kidney because these organs are the target of infection and play an important role in the immune status of the host. Results showed thatthesecondary infection by hydatid cyst fluid in the experimental model causedsevere pathological changes which increased in rats of groups 2 and 3 along with the time of infection, in addition to considerable changes that revealed by hematological and biochemical tests.The median IL-10 level in rats of group 1 was 6.77 pg/ml compared to 5.74 pg/ml in control group, while the third group recorded the highest level equal 10.45 (above 10.0 pg/ml). The rats of the second group reached to 9.11 pg/ml, but no more survival of rats above 10.0 pg/ml. Elevated IL-10 levels were correlated with poor survival. We concluded that the most effective stage of hydatid cyst development was the stage before transformation to calcareous phase and the most effective changes in serological, biochemical, immunological and pathological changes occur during this stage.

  1. Abdullah, B.H. (2007). Some Helminthes Parasitized on Stray Cats Feliscatus L. in Basrah and Epizootiological and Biological Studies on TaeniataeniaeformiBatsch, 1786, Ph.D. Thesis, College of Education, University of Basrah, (In Arabic).
  2. Aboelhadid S. M., El-Dakhlya, k., Tokuma, Y., Fukushi, H.,Hassanin, K. (2013). Molecular characterization of Echinococcus granulosus in Egyptian donkeys. Vet Parasitol. 193: 292– 296.
  3. Akhtar, T., Mubashra, T. and Anwar, Z. (1998). Haematological and biochemical changes in rabbits due to high doses of crude hydatid cyst fluid of sheep origin. Pakistan Vet J. 18 (2): 82-86.
  4. Al- Nasiri, F.S. (2006). Biological and immunological study of hydatid cyst formation in albino mice. Ph.D. Thesis, College of Education, University of Baghdad, (In Arabic).
  5. Allain, C., Poon, L., Chan, C., Richmond, W. and Fu, P. (1974). Enzymatic determination of total serum cholesterol. ClinChim. 20: 470- 475.
  6. Anwar, Z. and Tanveer, A. (2000). Blood biochemistry of rabbits after intravenous inoculation of unilocular hydatid cyst fluid of sheep origin. Punjab Univ J Zool. 15: 137- 149.
  7. Ayaz, E., Ertekin, A., Özdal, N. and Ta?, Z. (2007). Fasciola spp., Dicrocoeliumdendriticum, Kist Hidatik, TrichostrongylidaeveProtostrongylidaeKoyunlardaBaz?BiyokimyasalParametreler. TürkiyeParazitolojiDergisi. 30 (4): 57-61.
  8. Babba, H., Messedi, A., Masmoudi, S., Zribi, M., Grillot, R., Ambriose-Thomas, P., Beyrouti, I. and Sahnoun,(1994). Diagnosis of human hydatidosis: comparison between imagery and six serologic techniques. Am J Trop Med Hyg. 50:64-68.
  9. Balistreri, W. and Shaw, L. (1987). Liver function: Fundamentals of clinical chemistry. 3rd Philadelphia, 729-761.
  10. Bancroft, J .D. (1975). Histochemical techniques. 2nd, Butter worthies, London and Boston.
  11. Bayati, S., Aziz, O., Abdulla, A. and Abed, S. (2010). Biochemical profile of hydatid cyst fluids of Echinococcus granulosus of sheep in Duhok area. Iraqi J Vet Med. 34 (1): 78-83.
  12. Benjamin, M. (1985). Online of veterinary clinical parasitology. 1st Oxford University Press 38-162.
  13. Budke, C.M., Deplazes, P. and Torgerson, P.R. (2006). Global socioeconomic impact of cysticechinococcosis. EmergInfect Dis. 12(2): 296–303.
  14. Caraway, W. and Watts, N. (1987). Carbohydrates in fundamentals of clinical chemistry. 3ry ed. Philadelphia, 422-447.
  15. Carmena, D., Sánchez-Serrano, L. P. andBarbero-Martínez, I. (2008). Echinococcus granulosus infection in Spain.  Zoonoses and Public Health 55(3):156–165.
  16. Cheesbrough, M. (1998). District laboratory practice in tropical countries part 1.Cambridge University press, Cambridge 1: 355-358.
  17. Craig, P.S., McManus, D.P., Lightowlers, M.W., Chabalgoity, J.A., Garcia, H.H., Gavidia, C.M., Gilman, R.H., Gonzalez, A.E., Lorca, M., Naquira, C., Nieto, A. and Schantz, P.M. (2007). Prevention and control of cystic echinococcosis. Lancet Infect Dis. 7:385-394.
  18. Dacie, J. V. and Lewis, S. M. (1991). Practical Haematology. Churchill Livingstone. Edinburgh 7: 521- 534.
  19. Dakkak, A. (2010). Echinococcosis/hydatidosis: a severe threat in Mediterranean countries. Vet Parasitol. 174(1-2): 2–11.
  20. Dede, S., Altu?, N., De?er, Y., Özdal, N. and Ceylan, E. (2014). Serum biochemical profile and protein fractions in cattle with Theileriosis. Revue MédVét. 165 (56): 137-143.
  21. Doumas, B.,Waston, W. and Biggs, H. (1971). Albumin standard and measurement of serum albumin with bromocresol green. ClinChimActa. 31: 87- 96.
  22. El-Kattan, A.M. (2012). Some studies on hydatidosis in camels. Ph. D. V. Sc. Thesis Infectious Diseases. Fac. Vet. Med. Cairo University, Egypt.
  23. Finkelman, F.D., Pearce, J.F., Urban, J.R., and A. Sher.(1991). Regulation and biological function of helminth-induced cytokine responses. Immunol Today12:A62-A66.
  24. Frayha, G.J. and Haddad, P. (1980). Comparative chemical composition of protoscoleces and hydatid cyst fluid of Echinococcus granulosus. Int J Parasitol. 10: 359.
  25. Gella, F., Olivella, T., Cruz, M., Arenas, J., Durban, R. andComez, A. (1985). A simple procedure for routine determination of aspartate aminotransferase and alanine aminotransferase with pyridoxal phosphate. ClinChemActa. 153: 241- 247.
  26. Guyton, A. C. (1993). Textbook of Medical Physiology. 9" ed. W.B. Saunders Co., Philadelphia.
  27. Halsted, J.A. and Halsted, C.H. (1991). The laboratory in clinical medicine: Interpretation and application. Saunders Company, Philadelphia 2: 281-283.
  28. Haralabidis, S., Karagouni, E., Frydas, S. and Dotsika, E.(1995). Immunoglobulin and cytokine profile in murine secondary hydatidosis. Parasite Immunol. 17:625-630.
  29. Hillman, G. (1971). Continuous photometric measurement of prostrate acid phosphatase activity.Z KlinChemKlinBiochem.  9:273.
  30. Holcman, B. and Heath, D.D.(1997). The early stages of Echinococcus granulosus  Acta Trop. 64:5-17.
  31. Khalaf, A. (2013). Effects of hydatid cyst infection on some biochemical and haematological parameters in experimental mice Balb/ c strain. J College of Edu pure sci. 3(2): 1-9.
  32. Khalifa, N.o., Khater, H.F.,  Fahmy, H.A.,  Radwan,M.E. and Afify, J.S.A. (2014). Genotyping and phylogenetic analysis of cystic echinococcosis isolated from camels and humans in Egypt. Amer J EpidInfect Dis.  2.3 (2014): 74-82.
  33. Lange, A. M., Yutanawiboonchai, W., Scott, P. and Abraham, D. (1994). IL-4- and IL-5-dependent protective immunity to Onchocerca volvulusinfective larvae in BALB/c BYJ mice. J Immunol. 153:205-211.
  34. Macpherson, C. N. (1985). Echinococcosis a plague on pastoralist. In Parasitic Helminths and Zoonosis in Africa. 2: 25- 53.
  35. McManus, D. P. and Bryant, C.(1995). Biochemistry, physiology and molecular biology of Echinococcus,  355-410. In R. C. A Thompson and A. J. Lymbery (ed.), The biology of Echinococcus and hydatid disease. CAB International, Wallingford, United Kingdom.
  36. McManus, D. P. and Thompson, R. C. A. (2003). Molecular epidemiology of cystic echinococcosis. 127: S37–S51.
  37. Meduri, A., Lovane, A., Martone, A., Bonaduces, S. and Palomba, E. (1990). Sialic acid, mucoproteins and phospholipids in serum of healthy cattle with echinococcosis, and in hydatid fluid. Acta Medical Vet. 36(2): 151-166.
  38. Menezes da Silva, A. (2010). Human echinococcosis: a neglected disease.  Gastroenterology Research and Practicehttp://dx.doi.org/10.1155/2010/583297
  1. Mondragón-De-La-Peña, C., Ramos-Solís, S., Barbosa-Cisneros, O., Rodríguez-Padilla, C., Tavizón-García, P. and Herrera-Esparza, R. (2002). Echinococcus granulosus down regulates the hepatic expression of inflammatory cytokines IL-6 and TNF? in BALB/c mice.  Parasite 9(4): 351–356.
  2. Moraitaki, P., Roussi, P., Emmanouil, Y., Pechlivanidou, R., Bitsakou, C., Konstantinou, M. and Marosis, K. (2010). Pulmonary echinococcosis presenting as a pulmonary mass with fever and haemoptysis; a case report. Pneumon. J. 23(2): 180 – 183.
  3. Muhsin, S., Ahmed, D. and Hassan, A. (2015).  Hydatid cyst fluid of Echinococcus granulosus isolated from infected Human, sheep and cattle in Baghdad. J Nursing Health Sci. 4 (6):72-74.
  4. Nakaya, K., Mamuti, W., Xiao, N., Sato, M.O., Wandra, T.,Nakao, M., Sako, Y., Yamasaki, H., Ishikawa, Y., Craig,P.S., Schantz, P.M. and Ito, A. (2006). Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis. Parasitol Int. 55: 91–97.
  5. Namjoo, A. and Arzanipour, N. (2014). Serum liver enzymes profiles of cattle infected with hydatid cyst at Shahrekord abattoir in ChaharmahalVaBakhtiari Province, Iran. Int J Biosci. 4 (1): 469- 476.
  6. Orhue, N.E.J. andNwanze, E.A.C. (2004). Effect of Scopariadulcis on Trypoanosomabrucei induced alterations in serum transaminase, alkaline phosphatase and bilirubin in the rabbit. J Med Sci. 4(3): 194- 197.
  7. Orlowski, M. andMeister, A. (1963). Gamma-glutamyl-p-nitroanilide: a new convenient substrate for determination and study of l- and d-gamma-glutamyltranspeptidase activities. BiochimBiophys Acta.73(6): 679-681.
  8. Pearce, E. J., Caspar, P., Grzych, J.M., Lewis, F.A. and Sher, A.(1991). Down regulation of Th1 cytokine production accompanies induction of Th2 responses by a parasitic helminth, Schistosoma mansoni. J Exp Med. 173:159-166.
  9. Radfar, M., Tajalli, S. and Alipour, T.  (2012). Comparative study of cattle, sheep and goats on biochemical profiles of hydatid cyst fluid from the lungs and liver. Comp ClinPathol. 21: 1135–1137.
  10. Rahman, M.S., Sokar,S. and Dahab,  (1992).  Comparative studies on hydatidosis in farm animals in Egypt. DtschTierarztlWochenschr 99: 438-440.
  11. Rogan, M.T.(1988). Observations on the origin of daughter cysts within hydatid cysts of Echinococcus granulosus. Ann Trop Med Parasitol. 82:405-406.
  12. Richards, K. S., Arme, C. and Bridges, J.F.(1983). Echinococcus granulosus equinus: an ultrastructural study of murine tissue response to hydatid cysts.  86:407-417.
  13. Riley, E. M., Dixon, J. B., Jenkins, P. and Ross, G. (1986). Echinococcus granulosusinfection in mice: host responses during primary and secondary infection. Parasitol. 92:391-403.
  14. Riley, E.M., Dixon, J.B., Kelly, D.F. and Cox, D.A. (1985). The immune response to Echinococcus granulosus:sequential histological observations of lymphoreticular and connective tissues during early murine infection. J Comp Pathol. 95:93-104.
  15. Rosalki, S., Foo, A. and Burlina, A. (1993). Multicenter evolution of iso- ALP test kit for measurement of bone alkaline phosphatase activity in serum and plasma. Clin Chem. 39: 648- 652.
  16. Ruedai, M., Osunaz, A., Derycke, P. and Janssen, D. (1995). Biochemical evaluation of mice, experimentally infected with Echinococcus granulosus metacestodes. Res Rev ill Parasitol. 55 (2): 109-111
  17. Salih, M., Hailu, D. and MotiYohannes, M. (2011). Infection rates, cyst fertility and larval viability of hydatid disease in camels (Camelusdromedarius) from Borena, Kereyu and Harare areas of Ethiopia. Global Vet. 7(6): 518-522.
  18. Shaafie, A., Khan, A.H.  and  Rambabu, K. (1999). Biochemical profiles of hydatid cyst fluids of Echinococcus granulosus of human and animal origin in Libya. J Helminthol. 73(3): 255-263.
  19. Sharif, M., Keighobadi, M., Ziaee, H., Izadi, J., Gholami, S.H. and  Khalilian A.  (2004). Measurement of biochemical components of liver Hydatid cyst fluids in human, sheep, goat, cattle and camel. Mazandaran J Arak Uni Med Sci. 2: 24-31.
  20. Shindala, N. A., Al-Kassim, K. H., Shabaan, E. K., Shehab, A. K., Husain, A. K. and Alias, H. Y.  (2007). Study the toxic effect of albendazole excretion in the milk on the sucking pups. The 4th Scientific Conference. College of Veterinary Medicine, Mosul University 1:177-188.
  21. Siracusano, A., Rigano, R., Ortona, E., Profumo, E., Margutti, P., Buttari, B., Delunardo, F. and Teggi, A. (2008). Immunomodulatory mechanisms duringEchinococcus granulosus  ExpParasitol. 119:483–489.
  22. Siracusano, A.,  Delunardo, F.,  Teggi, A. and  Ortona, E. (2012). Host-Parasite Relationship in Cystic Echinococcosis: An evolving story clinical and developmental immunology.
http://dx.doi.org/10.1155/2012/639362.
  1. Smith, P.K. (1985). Measurement of protein using bicinchoninicacid.Anal Biochem. 150, 76-85.
  2. Smyth, J. D. (1979). Echinococcus granulosus and multilocularis: in vitro culture of strobilar stage from protoscolices. AngewParasit.  20:137-147.
  3. Smyth, J. and Barrett, N.J. (1980). Procedure for testing the viability of human hydatid cyst following surgical removal, especially after chemotherapy. Trans Royal Soc Trop Med Hyg. 74: 649–652.
  4. Smyth, J. and Davies, Z. (1974). In vitro culture of the strobilar stage of Echinococcus granulosus (sheep strain): A review of basic problems and results. Int J Parasitol. 4(6): 631-644.
  5. Tanveer, A., Ahmad, W. and Anwar, Z. (1997). Effect of low doses of filtered hydatid cyst fluid on some blood and liver function tests in rabbits. Biologia. 43:169-181.
  6. Thompson, R. C. A. (1977). Biology and Systematic of Echinococcus In “Biology of Echinococcus and Hydatid disease”. George Allen and Unwin, London 4: 52.
  7. Thompson, R. C. A. (1976). Biology and Systematics of Echinococcus. The Biology of Echinococcus and Hydatid Disease. Ed. Unwin, London 5: 43.
  8. Thompson, R. C. A. (1982). Biology and Systematic of Echinococcus and Hydatid disease. CAB International Wallingford, Oxen 1:37.
  9. Turgut, M. (2000). Hydatid disease of the spine: A survey study from Turkey. Inf. 25: 221–226.
  10. Varcasia, A. Tanda, B., Giobbe, M., Solinas, C., Pipia, A.P., and Malgor, R. (2011). Cystic echinococcosis in Sardinia: farmers’ knowledge and dog infection in sheep farms. Vet Parasitol. 181(2): 335-340.
  11. Wang, J., Xing, Y., Ren, B., Xie, W.D., Wen, H. and Liu, W.Y. (2011). Alveolar echinococcosis: correlation of imaging type with PNM stage and diameter of lesions. Chin Med J. 124 (18): 2824–2828.
  12. Xhemollari, E., Dhaskali, L., Papaioannou, N. and Kritsepi M. (2016). Evaluation of hematological and biochemical indicators by pathologies. The 4th Global Virtual Conference, 314-318.
  13. Yarsan, E., Altinsaat, C., Aycicek, H.,Sanindokuyucu, F. and Kalkan, F. (2003). Effect of albendazole treatment on hematological and biochemical parameters in healthy and Toxocaracanis infected mice. Turk J Vet Anim Sci. 27: 105- 117.
  14. Yeruham, I.,Hadani, A., Galker, F., Avidar ,Y. andBogin, E. (1998). Clinical, clinico-pathological and serological studies of Babesiaovis in experimentally infected sheep. ZentralblVeterinarmed B. 45(7): 385-394.
  15. Younus, M., Muhammad, M., Khan, A., Akhtar, T. and Akhtar, M. (2016). Hematological changes in the hydatidosedmale sheep after experimental inoculation of Echinococcus granulosus  Int J Agri Biosystems Eng.  3(1).
  16. Zak, O. and Sande, M.A. (1999). Hand book of animal models of infection. England 1:142-170.
  17. Zhang, W. B. and D. Z. Zhao.(1992). A comparison of calcification of hydatid cysts in sheep pastured and post-fed. Chin J Vet Sci Technol. 22:28-29.
  18. Zygner, W., Gojska, O., Rapacka, G., Jaros, D. and Wedrychowicz, H. (2007). Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland). Vet Parasitol. 145:146-151.

Rasha A. El Sayed, Safaa M. Barghash, Nadia M. El-Alfy, Basma M. Abou-Elnour and Al- Shaimaa M. Sadek. (2017); PHYSIOLOGICAL, IMMUNOLOGICAL AND HISTOPATHOLOGICAL COMPARISON OF ECHINOCOCCUS GRANULOSUS (G6) CAMEL STRAIN BY DIFFERENT VIABILITY STATUS USING SECONDARY CYST DEVELOPMENT IN RAT, Int. J. of Adv. Res., 5 (02), 2119-2131, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/3385

Safaa Mohamed Barghash
Post Doctor of Parasitology, Parasitology Unit, Animal Health Department, Desert Research Center (DRC). Phone: +202- 26332846, Fax: +202-26357858, B.O.P. 11753 Address: 1- Mathaf El-Mataria St, El-Naam, Cairo, Egypt. Mobile: +20 1067859132 E- mail: Barghash_7@yahoo.com

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Article DOI: 10.21474/IJAR01/3385      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3385

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