30Mar 2017

EFFECT OF FROZEN STORAGE AND CRYOPROTECTANTS ON FUNCTIONAL PROPERTIES OF TILAPIA (Oreochromis mossambicus) FISH

  • ICAR-Mumbai Research Centre of Central Institute of Fisheries Technology, CIDCO, Admin Bldg, Sector-1, Vashi, Navi Mumbai – 400703, Maharashtra, India.
  • Fisheries Research and Information Centre, Bhutanal, Vijayapura – 586103, Karnataka, India.
  • Fisheries Research and Information Centre (Inland), 10th cross, Mayura Street, Papanna Layout, Hebbal Outer Ring Road, Bangalore – 560 094, Karnataka, India.
  • Department of Fish Processing Technology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore - 575 002, India.
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In the present investigation, changes in functional properties of proteins from tilapia (Oreochromis mossambicus) in the form of whole tilapia (WHT), water washed minced meat of tilapia without addition of cryoprotectants (WMT) and with cryoprotectants (WMCT) during frozen storage have been evaluated. Gradual reduction in moisture content and marginal increase in protein content was observed in all the samples with progressive frozen storage. The reduction in non protein nitrogen (NPN) content was lower in WMCT as compared to WHT and WMT. All the samples exhibited decreased calcium-ATPase activity as a function of frozen storage. WMCT had a higher activity index during frozen storage as compared to WHT and WMT. The enzymatic activities decreased with progressive frozen storage period with relatively higher activity in WHT. Proteins from washed meat with cryoprotectants had minimum change in reduced viscosity profile at single protein concentration during different periods of frozen storage. Changes in structure of proteins were evident from apparent reduced viscosity and gel filtration profile as a function of frozen storage.

  1. Tan, A., Voegtline, M., Bekele, S., Chen, C.S. and Aroian, R.V. (2008): Expression of Cry5B protein from Bacillus thuringiensis in plant roots confers resistance to root-knot nematode. Biol. Control., 47: 97-102.
  2. Pandey, R. and Kalra, A. (2003): Root knot disease of ashwagandha Withania somnifera and its ecofriendly cost effective management. J. Mycol. Pl. Pathol., 33(2): 240-245.
  3. Charnley, A.K. (1992): Mechanisms of fungal pathogenesis in insects with particular reference to locusts. In: Lomer CJ, Prior C (eds) Biological Controls of Locusts and Grasshoppers: Proceedings of an international workshop held at Cotonou, Benin. Oxford: CAB International, pp. 181-190.
  4. An, H., Weerasinghe, V., Semour, T.A. and Morrissey, M.T. (1994): Cathepsin degradation of pacific whiting surimi proteins. Food Sci. 59(5): 1013-1033.
  5. 2000. Official methods of analysis of AOAC International, Horwitz, W. (eds.) AOAC International, Gaithersburg, Maryland, USA.
  6. Arakawa, T. and Timasheff, S.N. (1982): Preferential interactions of proteins with salts in concentrated solutions. Biochem. 21(25): 6545-6552.
  7. Arekere, G. (1993): Effect of freezing and frozen storage on the properties of proteins from common carp (Cyprinus Carpio). Post graduation dissertation, Univ. Agric. Sci., Bangalore, India.
  8. Badii, F. and Howell, N.K. (2001): A comparison of biochemical changes in cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) fillets during frozen storage. J. Sci. Food Agric. 82: 87-97.
  9. Basavanagouda, N. (2001): Setting ability of surimi from croaker (Johnius s) ?sh meat: Effect of frozen storage and added cryoprotectants. Post graduation dissertation, University of Agricultural Sciences, Bangalore, India.
  10. Binsi, P.K., Shamasundar, B.A. and Dileep, A.O. (2007): Physico-chemical and functional properties of proteins from green mussel (Perna viridis) during ice storage. J. Sci. Food Agric. 87: 245-254.
  11. Buttkus, H. (1970): Accelerated denaturation of myosin in frozen solution. J. Food Sci. 35: 558-562.
  12. Colmenero, J.F. and Borderias, A.J. (1983): A study of the effects of frozen storage on certain functional properties of meat and fish proteins. J. Food Technol. 18: 731-737.
  13. Fernandes, A.F.A., Silva, M.D., De-Alvarenga, E.R., Teixeira, E.D., Da-Silva, A.F. and Alves, G.F.D. (2015): Morphometric traits as selection criteria for carcass yield and body weight in Nile tilapia (Oreochromis niloticus) at five ages. Aquacult. 446: 303-309.
  14. Fogaca, F.H., SantAnac, L.S., Larab, J.A.F., Maia, A.C.G. and Carneiroc, D.J. (2015): Restructured products from tilapia industry byproducts: The effects of tapioca starch and washing cycles. Food Bioprod. Proc. 94: 482-488.
  15. Ganesh, A., Dileep, A.O., Shamasundar, B.A. and Singh, U. (2006): Gel forming ability of common carp fish (Cyprio carpio): effect of freezing and frozen storage. J. Food Biochem. 30: 342-361.
  16. Herrera, J. and Sampedro, L. (2002): Effects of various cryostabilisers on protein functionality in frozen-stored minced blue whiting muscle: The importance of inhibiting formaldehyde production. Eur. J. Food Res. Technol. 214: 382-387.
  17. Jackson, C.T., Acuff, R.G. and Dickson, J.S. (1997): Meat, poultry and seafood. In: Food Microbiology, fundamentals and frontiers. Doyle, M.P., Beuchat, L.R., and Montville, T.J. (Ed.) American Society for Microbiology, Washington. pp. 263-300
  18. Karthikeyan, M., Dileep A.O. and Shamasundar B.A. (2006): Effect of water washing on the functional and rheological properties of proteins from threadfin bream (Nemipterus japonicus) meat. Int. J. Food Sci. Technol. 41: 1002-1010.
  19. Karthikeyan, M., Shamasundar, B.A., Mathew, S., Ramesh Kumar, P. and Prakash, V. (2004): Physico-chemical and functional properties of proteins from pelagic fatty fish (Sardinella longiceps) as a function of water washing. Int. J. Food Prop. 7(3): 353-365.
  20. Ko, aC. and Hsu, K.C. (2002): Effect of high-pressure storage on the processing quality of tilapia meat. Biotechnol. Prog.a 19: 411-416.
  21. Lehninger, A.L. (1993): The Molecular Basis of Cell Structure and Function. 2nd Worth Publ. Inc., New York. pp. 1104.
  22. Lin, T.M. and Park, J.W. (1998): Solubility of salmon myosin as affected by conformational changes at various ionic strengths and pH. Food Sci. 63: 215-218.
  23. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
  24. Murthy, L.N. and Rajanna, K.B. (2011): Effect of washing on composition and properties of proteins from Tilapia (Oreochromis mossambicus) meat. Fish. Technol. 48(2): 125-132.
  25. Murthy, L.N., Panda, S.K. and Shamasundar, B.A. (2011): Physico-chemical and functional properties of proteins of tilapia (Oreochromis mossambicus). Food Proc. Engg. 34: 83-107.
  26. Ng, W.K. and Bahurmiz, O.M. (2009): The impact of dietary oil source and frozen storage on the physical, chemical and sensorial quality of fillets from market-size red hybrid tilapia, Oreochromis Food Chem. 113: 1041-1048.
  27. Nishiya, K., Takeda, F.K., Tamoto, O., Tanaka, T., Fukumi T., Yashi K. and Aizawa, S. (1961): Studies on freezing of surimi (fish paste) and its applications (IV): on freezing surimi of Atka mackerel meat. Mon. Rep. Hokkaido Mun. Fish. Exp. Stn. 18: 391-397.
  28. Noguchi, S. and Matsumoto, J.J. (1970): Studies on the control of the denaturation of fish muscle properties during frozen storage-I. Preventive effect of Na-glutamate. Bull. Jap. Soc. Sci. Fish. 36: 1078-1087.
  29. Nopianti, R., Huda, N., Fazilah, A., Ismail, N. and Easa, A.M. (2012): Effect of different types of low sweetness sugar on physicochemical properties of threadfin bream surimi (Nemipterus ) during frozen storage. Int. Food Res. J. 19(3): 1011-1021.
  30. Pan, J., Shen, H. and Luo, Y. (2010): Cryoprotective effects of trehalose on grass carp (Ctenopharyngodon idellus) surimi during frozen storage. J. Food Proc. Preserv. 34: 715-727.
  31. Park J.W., Korhonen, R.W. and Lanier, T.C. (1990): Effect of rigor mortis on gel forming properties of surimi and unwashed mince prepared from Tilapia. J. Food Sci. 55: 335-360.
  32. Park, J.W. and Lin, T.M.J. (2005): Surimi: Manufacturing and Evaluation. In: Surimi and Surimi seafood. 2nd Park, J.W. (Eds.) Taylor and Francis, Boca Raton. pp. 33-40.
  33. Park, J.W. and Lin, T.M.J. (2013): Surimi: Manufacturing and Evaluation. In: Surimi and Surimi seafood. 3rd Park, J.W. (Ed.) Taylor and Francis, Boca Raton. pp. 21.
  34. Shenouda, S.Y.K. (1980): Protein denaturation in frozen fish. Adv. Food Res. 26: 275-311.
  35. Siddaiah, D., Reddy, G.V.S., Raju, C.V. and Chandrasekar, T.C. (2001): Changes in lipids, proteins and Kamaboko forming ability of silver carp (Hypopthalmichthys molitrix) mince during frozen storage. Food Res. Int. 34: 47-53.
  36. Suvanich, V., Jahncke, M.L. and Marshall, D.L. (2000): Changes in selected chemical quality characteristics of channel catfish frame mince during chill and frozen storage. J. Food Sci. 65: 24-29.
  37. Suzuki, T. (1981): Fish and Krill protein: Processing Technology, Applied Science Publishers: Barking, Essex.
  38. Taussky, H.H. and Shorr, E. (1952): A micro colorimetric method for the determination of inorganic phosphorus. J. Biol. Chem. 202: 675-685.
  39. Velankar, N.L. and Govindan, T.K. (1958): A preliminary study of the distribution of non-protein nitrogen in some marine fishes and invertebrates. Proc. Ind. Acad. Sci. 47: 202-209.
  40. Venugopal, V. and Shahidi, F. (1998): Traditional methods to process underutilized fish species for human consumption. Food Rev. Int. 14: 35.
  41. Yang, J.T. (1961): The viscosity of macromolecules in relation to molecular conformation. Adv. Prot. Chem. 16: 323-400.
  42. Yongsawatdigul, J., Park, J.W., Virulhakul, P. and Viratchakul, S. (2000): Proteolytic degradation of tropical tilapia surimi. Food Sci. 65(1): 129-133.
  43. Yoon, K.S., and Lee, C.M. (1990): Cryoprotectant effects in surimi and surimi/mince-based extruded products. J. Food Sci. 55(5): 1210-1216.
  44. Zhou, A., Benjakul, S., Pan, K., Gong, J. and Liu, A. (2006): Cryoprotective effects of trehalose and sodium lactate on tilapia (Sarotherodon nilotica) surimi during frozen storage. Food Chem. 96: 96-103.

[Lakshmi Narasimha Murthy, Girija Gajanan Phadke, Vijaya Kumar Siddaiah, Rajanna Karani Boraiah and Chandra Madhamuthanalli Venkataramappa. (2017); EFFECT OF FROZEN STORAGE AND CRYOPROTECTANTS ON FUNCTIONAL PROPERTIES OF TILAPIA (Oreochromis mossambicus) FISH Int. J. of Adv. Res. 5 (Mar). 1597-1606] (ISSN 2320-5407). www.journalijar.com

Dr. L. Narasimha Murthy
ICAR-Mumbai Research Centre of Central Institute of Fisheries Technology, CIDCO, Admin Bldg, Sector-1, Vashi, Navi Mumbai – 400703, Maharashtra, India

DOI:

Article DOI: 10.21474/IJAR01/3668      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3668

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