ALPHA AMYLASE INHIBITION ACTIVITY OF OKRA MUCILAGE.
- Dept. of Biotechnology, Shivchhatrapati college,Aurangabad, M.S.India.
- Abstract
- Keywords
- References
- Cite This Article as
- Corresponding Author
In the present study the aqueous seed extract of okra Mucilage was used to examined for alpha amylase inhibition activity. Different concentrations of extracts were incubated with enzyme substrate solution and the activity of enzyme was measured. The maximum inhibition activity was observed at 240?l concentration. The starch agar plate method was also prepared for detection of alpha amylase inhibitor. This inhibitory compound also has insecticidal activity. Diabetes Mellitus is a metabolic disorder characterized by high blood glucose level caused due to deficiency of insulin action. The inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase can be important strategy in blood glucose level in patients with type II diabetes. In this study male mice were used to measure blood glucose level. The results show that the anti-amylase inhibitor was very powerful to reduce the level of glucose in the blood of the treated mice to the normal comparison with the control.The inhibitory compound was analyzed by FT-IR and SDS-PAGE.
- Abdulwahid h ,et al. (2014).? Studing the effect of anti amylase inhibitor extracted from white kidney bean (phaseolus vulgaris) in treat diabetes and obesity in an affected mice,International Journal of Current Microbiology and Applied Sciences vol.(3).
- Chauhan DVS , (1972) vegetable production in India, Ram Prasad and sons, Agra.
- Grubben GTH,(1977). Tropical vegetables and their genetic recources (Tindall H,D. and Willams J.T.eds.) IBPGER,Rome, ,pp197.
- Hamon, (1987). organization genetique dugenera Abelmos chees, Orstom paris,pp217.
- Kartic, et al .(2008). Identification of alpha amylase inhibitors from Syzygium cumini Linn seeds,Indian Journal of Experimental Biology,pp 677-680.
- Peyman Salehi,et al.(2013). Alpha glucosidase and alpha amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for Diabetes,Journal of Medicinal plants Recearch vol.7(6),pp257.
- Sharma S.B., et al.(2006). Anti hyperglycemic effect of fruit pulp of Eugenia jambolana in experimental Diabetes mellitus ,J Ethanopharmacol,104.
- Sabitha , et al (2012) . In Vitro alpha glucosidase and alpha amylase enzyme inhibitory effects in aqueous extracts of Abelmoscus esculentus(L.) Moench,Asian Pacific Journal of Tropical Biomedicine.
- Vijai sing, et al.(2012). An optimized method of DNA isolation from highly mucilage rich Okra for PCR analysis,Advances in Applied Science Rsearch,3(3),1809-1813.
- Vrun Kumar Prabhakar, et al. (2013). In Vitro Study Of Alpha amylase Inhibitory Activity and Phytochemical Screening Of Few Indian Medicinal Plant Having Antidibetic Properties,International Journal Of Scientific and Research publications.vol(3).
- Xiao-shun, et al.(2009). Anti hyperglycemic effects of total flavonoids from Polygonalum odoralum in STZ and alloxan induced diabetic rats,Journal of Ethnopharmacolo.
- Yoon S.H.,et al.(1969). Study of the inhibition of four alpha amylases by acarbose and it?s 4IV-alpha maltohexosyl and 4IV alpha malt tododecaosyl analogues, carbohydrates RES, 338(2003).
- Zohere Mirzaalian Dastjerdi et al.(2015). Alpha Amylase Inhibition Activity of some plants Extracts of Teucrium species, European Journal of Biological Sciences 7(1),26-31.`
[Manisha N. Chalse, Vijay Bondre and Sheetal Wahule. (2017); ALPHA AMYLASE INHIBITION ACTIVITY OF OKRA MUCILAGE. Int. J. of Adv. Res. 5 (Apr). 686-691] (ISSN 2320-5407). www.journalijar.com
Dept. of Biotechnology, Shivchhatrapati College, AURANGABAD
