25Apr 2017

DETERMINING THE EFFECT OF AFLATOXIN B1 USING HEMOGLOBIN OF HUMAN BLOOD AS INTERACTING MEDIUM BY CALCULATION OF COLLISION ENERGY.

  • Junior Research Fellow,Department of Physics, Indian Institute of Engineering Science and Technology.
  • Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology.
  • Associate Professor, Department of Physics, Indian Institute of Engineering Science and Technology.
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The objective of this study concerns whether there is any biochemical changes of the Red Blood Corpuscles (RBC) of human blood by using different concentrations (in micro molar) of Aflatoxin B1 (AflB1) and to determine the changes using the collision energy. RBC from the human blood was treated by AflB1at different time interval and the respective samples have been characterized by optical microscope followed by UV-Vis spectrophotometer and Fourier Transform Infra Red spectroscopy (FTIR). From the optical microscope data it has been noticed that the structure of the RBC is altered which is supported by the scanning electron microscope (SEM) pronouncedly. In the results studied by FTIR, some unconventional peaks have found and from these data we conclude about the typical bonding nature between the atoms involving in Hb and AflB1 chemical structure. The bonding of the newly formed structure is quite similar to that of the Aflatoxin G1 (AflG1) and after a period of time again the structure of the AflB1 (which initially added to the sample) returns back. According to our study the phenomena relating to the returning of the AflB1 structure is not only a factor of concentration of AflB1 added but also depends upon the duration of biochemical interaction of AflB1 with Hb. The change in collision energy due to structural deformation of Aflatoxin can easily be calculated from FTIR spectra data.

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[Bhadra Preetha, Das Mukhopadhyay Chitrangada and Mukherjee Sampad. (2017); DETERMINING THE EFFECT OF AFLATOXIN B1 USING HEMOGLOBIN OF HUMAN BLOOD AS INTERACTING MEDIUM BY CALCULATION OF COLLISION ENERGY. Int. J. of Adv. Res. 5 (Apr). 742-748] (ISSN 2320-5407). www.journalijar.com

Sampad Mukherjee
Department of Biotechnology, Government of India

DOI:

Article DOI: 10.21474/IJAR01/3871      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3871

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