THE EFFECT OF NICKEL CHLORIDE (NICL2) ON VARIOUS CALLUS GROWTH DYNAMICS OF COLEUS BLUMEI (BENTH.) CULTURES.

  • Department of Botanical and Environmental Sciences, Guru Nanak Dev University, 143005, Amritsar.
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An important member of family Lamiaceae, Coleus blumei has been priced for its medicinal and ornamental values. Due to phytotoxic effects of the heavy metal Nickel its impact on the growth rate of in vitro callus cultures has been studied. At different levels of 0.0 mM, 0.1 mM, 0.2 mM, 0.4 mM, 0.6 mM and 0.8 mM Ni concentrations were added to the Murashige and Skoog Medium. The data showed that there was a marked decrease in the callus growth parameters such as percent callus survival, percent callus multiplication, average fresh weight, average dry weight and percent growth index of callus. On the other hand, there was an increase in the percent callus dry matter content with respect to the control. The level of 0.4 mM concentration is considered to the inhibitory concentration (IC50) as there was 50% decrease in the growth dynamics of callus.

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Amandeep Chabhal and Gurdeep Singh Virk. (2017); THE EFFECT OF NICKEL CHLORIDE (NICL2) ON VARIOUS CALLUS GROWTH DYNAMICS OF COLEUS BLUMEI (BENTH.) CULTURES., Int. J. of Adv. Res., 5 (04), 863-868, ISSN 2320-5407. DOI URL: https://dx.doi.org/10.21474/IJAR01/3888

Prof. Gurdeep Singh Virk
Guru Nanak Dev University

DOI:

Article DOI: 10.21474/IJAR01/3888      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3888

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