Vol. 5 (04) pp. 1003-1009 DOI: 10.21474/IJAR01/3908

IMUNNOLOGICAL AND STRUCTURAL ANALYSIS OF HPV-POSITIVE CERVICAL CARCINOMA CELL LINES AND BOVINE PAPILLOMAVIRUS VIRUS-LIKE PARTICLES (BPV-VLP).

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  • Laboratory of Morphology and Viral Morphogenesis, Instituto Oswaldo Cruz, Funda??o Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, 21040-900, Rio de Janeiro, RJ, Brazil.
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Abstract

This paper reported about morphological alterations occurring inside HPV-positive cervical carcinoma cell lines and BPV infection. The wart samples were collected from animals affected by cutaneous papillomatosis and previously detected using PCR. The ultrastructural cell morphology in SiHa (HPV-16) and HeLa (HPV-18) cell lines (3x106 cells) detected by electron microscopy were also investigated. Few studies have assessed the transmission electron microscopy in different cell lines. Morphologically, very electron-dense cells were detected presenting well developed mitochondria and rough endoplasmic reticulum (rER), many vesicles and ribosomes in HeLa and SiHa cell lines. Cellular modifications similar to antigen-presenting cells, many activated mitochondria and vesicle transport were well preserved. Furthermore, the presence of VLP and cellular junctions like desmosomes were also detected in BPV samples. These morphological alterations suggest a high cellular activity of HPV-positive (SiHa and HeLa) cell lines and can be possible prognostic markers of cervical cancer. These results point to the potential to induce tumors that probably involves mechanisms of oncoproteins to produce virions and differentiation of keratinocytes in the epithelial layers remains to be elucidated.

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How to Cite This Article

Dra. Rachel Siqueira de Queiroz Simoes and Dra. Ortrud Monika Barth. (2017); IMUNNOLOGICAL AND STRUCTURAL ANALYSIS OF HPV-POSITIVE CERVICAL CARCINOMA CELL LINES AND BOVINE PAPILLOMAVIRUS VIRUS-LIKE PARTICLES (BPV-VLP). , Int. J. of Adv. Res., 5 (04), 1003-1009, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/3908

Corresponding Author

Dra. Rachel Siqueira de Queiroz Simões
LaboratoryofMorphologyand Viral Morphogenesis
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