25Apr 2017

A COMPARISON OF NEW SPERM PREPARATION TECHNIQUE BY GLASS WOOL FILTRATION COMBINED WITH PENTOXIFYLLINE WITH OTHER TECHNIQUES IN ASTHENOZOOSPERMIC MEN.

  • High Institute for Infertility Diagnosis and Assisted Reproductive Technologies , Al-Nahrain University, Baghdad-IRAQ.
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Background: Variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive technologies. Objectives: This study is designed to compare the asthenozoospermic semen outcomes of four in vitro sperm activation techniques and evaluate their efficiency, namely; Simple layer technique, Centrifugation technique ,Glass wool filtration technique and a new technique of Glass wool filtration combined with Pentoxifylline. Subjects, Materials and Methods: Forty infertile men with asthenozoospermia were involved in this study. Sperm parameters were assessed according to WHO (2010 and 1999). Post- activation of each sample were divided into four aliquots, the first one :using Simple layer technique , the second :using Centrifugation technique ,the third :Glass wool filtration technique , and last one :using Glass wool filtration technique with Pentoxifylline. Results: A significant increase of sperm motility, progressive sperm motility and morphologically normal sperm when using Glass wool filtration technique with Pentoxifylline as compared to Glass wool filtration technique. Also, there was a significant increase for the same parameters when using Glass wool filtration technique as compared to the Simple layer and Centrifugation techniques. Regarding sperm agglutination and Round cells count the study showed the best results by decreasing their levels when using Glass wool filtration combined with Pentoxifylline as compared to other techniques. Conclusions: From the results of the present study, the sperm function parameters outcome when using glass wool filtration technique combined with Pentoxifylline were superior to other techniques since it yields better sperm function parameters rather than the outcome of glass wool filtration, centrifugation and simple layer techniques; respectively especially when using a low quality of semen samples such as decreased sperm function parameters (sperm motility) so it was fit in cases of asthenozoospermia which taken as an example of infertile group within this study.

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[Ali A. Kadhim, Hayder A. L. Mossa , Muayad S. Abbood. (2017); A COMPARISON OF NEW SPERM PREPARATION TECHNIQUE BY GLASS WOOL FILTRATION COMBINED WITH PENTOXIFYLLINE WITH OTHER TECHNIQUES IN ASTHENOZOOSPERMIC MEN. Int. J. of Adv. Res. 5 (Apr). 1178-1182] (ISSN 2320-5407). www.journalijar.com

Assist.Prof.Dr.Hayder A.L. Mossa
Department of Clinical Reproductive Physiology-High Institute For Infertility Diagnosis and Assisted Reproductive Technologies-AL-Nahrain University-Baghdad-IRAQ

DOI:

Article DOI: 10.21474/IJAR01/3931      
DOI URL: https://dx.doi.org/10.21474/IJAR01/3931

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