20May 2017

SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN PAGE FAMILY MEMBER 4(PAGE4) GENE IN MEN WITH BENIGN PROSTATE HYPERPLASIA FROM IRAQ.

  • Medical Tools Department, Director of Materials Researches, Ministry of Science and Technology, Baghdad, Iraq.
  • Department of Urology, Al-Yarmook Teaching Hospital, Baghdad, Iraq
  • Medical and Molecular Biotechnology Department, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
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This study was aimed todetermine the single nucleotide polymorphisms (SNPs) in PAGE family member 4 (PAGE4) gene in men with benign prostate hyperplasia from Iraq. Blood samples were collected and molecular analysis of PAGE4 has been studied by using PCR. A primer was designed for amplification of first region which included (exon1, intron1, exon2 and part of intron2 ) of that gene. It was found that this region of gene appeared as a single band, 1491bp in size. Single nucleotide polymorphisms (SNPs) were determined in this regionusing DNA sequencing technique. Then, nucleotide sequences were aligned with control group (healthy men) and with NCBI. Results also showed that seven polymorphisms were detected in first region of PAGE4 gene; six of them were substitution polymorphisms while one was addition polymorphism. Upon such findings, it can be concluded that some single nucleotide polymorphism in PAGE4 gene may affect gene expression.

  1. Auffenberg, G., Helfan, B. and McVary, K. (2009). Established medical therapy for benign prostatic hyperplasia. Urol.Clin. North Am., 36: 443.
  2. Bechis, K..,Alexander, M.,Otsetov, G.and Rongbin, G.(2014).MD: Personalized Medicine for Management of Benign Prostatic Hyperplasia Urol.;192(1): 16?23.
  3. Cardiol, R.( 2014).Gene polymorphisms in Human. Human Gen., 22(1):1-12.
  4. Helfand, B., Hu, Q. and Loeb, S. (2013). Genetic sequence variants are associated with severity of lower urinary tract symptoms and prostate cancer susceptibility. Urol., 189:845.
  5. Lindstrom, S., Schumacher, F. andSiddiq, A.(2011). Characterizing associations and SNP-environment interactions for GWAS-identified prostate cancer risk markers--results from BPC3. PLoS One. 6:17142.
  6. Mullins, C., Lucia, M. and Hayward, S. (2008). A comprehensive approach toward novel serum biomarkers for benign prostatic hyperplasia: the MPSA Consortium. Urol. 179:1243.
  7. Roehrborn, (2012). Benign Prostatic Hyperplasia: Etiology, Pathophysiology, Epidemiology, and Natural History. In: Wein, A. J., Kavoussi, L. R., Partin, A. W., Peters, C. A., Novick, A. C., editors. Campbell-Walsh Urology. 10th ed. Philadelphia: Saunders; 2570−610.
  8. Rohrmann, S., Fallin, M. and Page, W. (2006). Concordance rates and modifiable risk factors for lower urinary tract symptoms in twins. 17:419.
  9. Sudmant, H., Kitzman, O., Antonacci,F.,? Alkan, C. and? Malig, M. (2010). Diversity of Human Copy Number Variation and Multi-copy Genes.; 330(6004): 641?646.
  10. Zeng, Y., Gao, D.,? Kim, J., Shiraishi, T. and Terada, N. (2013) . Prostate-associated gene 4 (PAGE4) protects cells against stress by elevating p21 and suppressing reactive oxygen species production.? J. Clin. Exp. Urol.;1:39?52.

[Linda,F. Abdul-Sattar, Hayder,I. Al-Ameery and Abdulwahid, B. Al- Shaibani. (2017); SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN PAGE FAMILY MEMBER 4(PAGE4) GENE IN MEN WITH BENIGN PROSTATE HYPERPLASIA FROM IRAQ. Int. J. of Adv. Res. 5 (May). 562-567] (ISSN 2320-5407). www.journalijar.com

LINDA, Fawzi ABDUL-SATTAR
1Medical Tools Department, Director of Materials Researches, Ministry of Science and Technology, Baghdad, Iraq.

DOI:

Article DOI: 10.21474/IJAR01/4146      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4146

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