27May 2017

INHIBITION OF ISPD ENZYME TO CONTROL THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS.

  • Post Graduate Centre & Department of Botany and Biotechnology, College of Commerce, Arts & Science, Patna (Bihar), India 800 020.
  • Intermediate Reference Laboratory, TBDC Campus, Agamkuan, Patna, Bihar 800 007.
  • Ashayan, D.P.R. Colony, Ramjaipal Road, Off West Bailey Road, P.O. Danapur (Bihar) Patna 801503.
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Like several other pathogens, Mycobacterium tuberculosis synthesizes isopentenyl diphosphate via the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. IspD (2C-methyl-D-erythritol-4-phosphate Cytidyltransferase) is a flexible enzyme that carries out the third step in MEP pathway where 2-C-methyl-D-erythritol 4 phosphate (MEP) condenses with Cytidine Triphosphate (CTP) to form 4-diphosphocytidyl-2-amethyl-D-erythritol (CDP-ME). It catalizes the transfer of a cytidyl group from CTP to methylerythritol phosphate and utilizes two substrates, MEP and CTP. Due to this feature, this stage of MEP pathway is a significant reaction step that can be targeted to control the spread of the organism in vivo. Rosuvastatin successfully docks as a ligand close to the active site of the IspD with involvement of low (negative) energy indicating a stable system and a likely binding interaction. It can, thus, be safely assumed that rosuvastatin molecule could possibly be used as a candidate to competitively inhibit IspD to bind to CTP blocking the MEP pathway and keep the proliferating Mycobacterium tuberculosis under control.

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[Manoj Kumar, Pratik Kumar, Pratima Kumari and Jainendra Kumar. (2017); INHIBITION OF ISPD ENZYME TO CONTROL THE GROWTH OF MYCOBACTERIUM TUBERCULOSIS. Int. J. of Adv. Res. 5 (May). 1309-1313] (ISSN 2320-5407). www.journalijar.com

Jainendra Kumar
College of Commerce, Arts & Science, Patna, Bihar, India 800 020.

DOI:

Article DOI: 10.21474/IJAR01/4249      
DOI URL: https://dx.doi.org/10.21474/IJAR01/4249

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