30May 2017

A COMPARATIVE STUDY ON QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITYOF VARIOUS SOLVENT EXTRACTS OF TETRACERA AKARA(BURM. F.) MERR., AN ETHNOMEDICINAL PLANT USED BY KANI TRIBE OF KERALA.

  • Ethnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical BotanicGarden and Research Institute, Palode, Thiruvananthapuram 695 562, Kerala, India.
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Tetracera akara(Burm. f.) Merr. (Dellineaceae) is a woody climber distributed in Indo-Malayan archipelago and in India, it is found in the Western Ghats region of Kerala and Tamil Nadu. The root of T. akara is used by the Kani tribe of Kerala, India to cure various liver ailments like jaundice, inflammatory conditions, fever and also to enhance the immunity in children.The present study was aimed to scientifically validate the free radical scavenging potential of different solvent extracts of T. akara roots with different in vitro assays and also to estimate the total phenolic, flavonoid and condensed tannin contents. A significantly higher total poly phenolic content was estimated in the ethanolic fraction ofroot ofT. akara. The aqueous extracts possess significantly higher phenol and flavonoid content. Theethanolic fraction ofT. akararootexhibited 86 % inhibition against DPPH radicals at 320?g/ml concentration. Hexane and chloroform fractions showed comparatively less DPPH radical activity. In nitric oxide radical scavenging assay, all the extracts except hexane fraction showed potent activity against the free radicals and in superoxide radical scavenging assay the maximum activity was exhibited by the ethanolic fraction when compared to the standard ascorbic acid. In the present study, the antioxidant potential and free radical scavenging effects of T. akaramay be attributed to the high polyphenolic content.

  1. Al-Mamun, M., Yamak, K., Masumizu, T., Nakai, Y., Saito, K. and Sano, H. (2007). Superoxide Anion Radical Scavenging Activities of Herbs and Pastures in Northern Japan Determined Using Electron Spin Resonance Spectrometry. Int. J. Biol. Sci. 3: 349-355.
  2. Blois, M.S. (1958). Antioxidant determination by the use of stable free radicals. Nature. 181, 1199-1200.
  3. Blokhina, O., Virolainen, E. and Fagerstedt, K. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany. 91: 179-194.
  4. Chang, C., Yang, M., Wen, H. and Chern, J., (2002). Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. J. Food Drug Analaysis. 10:178-182.
  5. Chung, K.T., Wong, T.Y., Huang, Y.W. and Lin, Y.? (1998). Tannins and human health: a review, Crit. Rev. Food Sci. Nutr. 38: 421-464.
  6. Cook, N. C. and Samman, S. (1996). Flavonoids chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem. 7: 66-76.
  7. Ebrahimzadeh, M.A., Nabavi. S.M., Nabavi. S.F., Eslami, B. and Ehsanifar, S. (2009). Antioxidant activity of Hyoscyamussquarrosus Pharmacologyonline. 2: 644-650.
  8. Fu, W., Chen, J., Cai, Y., Lei, Y., Chen, L., Pei, L., Zhou, D., Liang, X. and Ruan, J. (2010). Antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective potential of the extract from Parathelypterisnipponica (Franch. Et Sav.) Ching. J. Ethnopharmacol. 130(3), 521-528.
  9. Hagerman, A.E., Riedl, K.M., Jones, G.A., Sovik, K.N., Ritchard, N.T., Hartzfeld, P.W. and Riechel, T.L. (1998). High molecular weight plant polyphenolics (tannins) as biological antioxidants. J. Agric. Food Chem. 46: 1887-1892.
  10. Halliwell, B. and Gutteridge, J. M. C. (1998). Free radicals in biology and medicine. London: Oxford University Press.
  11. Harput, U. S., Gen?, Y., Khan, N. and Saracoglu, I. (2011). Radical scavenging effects of different Veronica Records of Natural Products. 5 (2): 100-107.
  12. Kessler, M., Ubeaud, G. and Jung, L. (2003). Anti and pro-oxidant activity of Rutin and Quercetin derivatives. Pharm.&Pharmacol. 55: 131-142.
  13. Lima, C. C., Lemos, R. P. L. and Conserva, L. M. (2014). Dilleniaceae family: an overview of its ethnomedicinal uses, biological and phytochemical profile. J.Pharmacogn.Phytochem. 3(2): 181-204.
  14. Mondal, S.K., Chakraborty, G., Gupta, M. and Mazumder, U.K. (2006). In vitro antioxidant activity of DiospyrousmalabaricaKostel bark. Ind. J. Exp. Biol. 44 (1), 39-44.
  15. Nabavi, S. M., Ebrahimzadeh, M. A., Nabavi, S. F., Hamidinia, A. and Bekhradnia, A. R (2008). Determination of antioxidant activity, phenol and flavonoid content of Parrotiapersica Pharmacologyonline. 2: 560-567.
  16. Nagulendran, K.R., Velavan, S., Mahesh, R. and Begum, V.H. (2007). In vitro antioxidant activity of and total phenolic content of Cyperusrotundus E-Journal of Chemistry. 4: 440-449.
  17. Osawa, T., Uritani, I., Garcia, V.V. and Mendoza, E.M. (1994) Novel neutral antioxidant for utilization in food and biological systems. Japan: Japan Scientific Societies Press; p.p 241-251.
  18. R. Nair, Suja, S. R., Vilash, V., Aneeshkumar, A. L. and Rajasekharan. S. (2016). Pharmacognosticstandardization and phytochemical analysis of Tetracera akara (Burm. f.) Merr. J. Trad. & Folk Pra. Volume 04(2): 79 - 95
  19. Saradamma, L., Nair, C. R., Bhat, A. V. and Rajasekharan, S. (1994). Final technical Report - All India Co-Ordinate Research Project?on Ethnobiology Phase II.
  20. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphor molybdic phosphor tungstic acid reagents. Am. ? Enol.? Vitic. 16: 144-158.
  21. Sulaiman, S., Ibrahim, D., Kassim, J., Hong, L.S. (2011). Antimicrobial and antioxidant activities of condensed tannin from Rhizophoraapiculata bark, J. Chem. Pharm. Res.3: 436-444.
  22. Swapna, T.S., Archana, G.N., Mini, I. and Pradeesh, S. (2014). Free radical scavenging activity of leaves of Bidensbiternata (Lour.) Merr. & Sheriff, Int. J. of Pharm. Res.&Dev.6: 127-135.
  23. Udayan, P. S. and Indira, B. (2009). Medicinal plants of Arya Vaidya Sala Herb Garden, Dept. of Publications, Arya Vaidya Sala, Kottakal, Kerala-676503. Kottakkal Ayurveda Series.72: 345-346
  24. Wong, C.C., Li, H.B., Cheng, K.W. and Chen, F.? (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem. 97: 705-711.
  25. Yen, G.C., Duh, P.D. and Tsai, C.L. 1993. Relationship between antioxidant activity and maturity of peanut hulls. J. Agric. Food Chem. 41: 67-70.

[Ragesh R Nair, S R Suja, A L Aneeshkumar, V Vilash and S Rajasekharan. (2017); A COMPARATIVE STUDY ON QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITYOF VARIOUS SOLVENT EXTRACTS OF TETRACERA AKARA(BURM. F.) MERR., AN ETHNOMEDICINAL PLANT USED BY KANI TRIBE OF KERALA. Int. J. of Adv. Res. 5 (May). 1907-1915] (ISSN 2320-5407). www.journalijar.com

Dr. S R. Suja
Ethnomedicine and Ethnopharmacology Division, JNTBGRI, Palode

DOI:

Article DOI: 10.21474/IJAR01/4328      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4328

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