COLUMN CHROMATOGRAPHY FRACTIONAL ANALYSIS OF ERYTHRINA VARIEGATA L. LEAF EXTRACT FOR ITS ANTIBACTERIAL EFFICACY.
- P.G Department of Biotechnology, A.N. College, Magadh University, Patna.
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Medicinal Plants are source of many noble drugs compound. Secondary metabolites are a source of biological active natural products with high potential to act as antibacterial and also as inhibitors and plant growth promoters. The progress in this area has great potential for industrial application especially in pharmaceuticals and nutraceuticals. This medicinal plants show the presence of Alkaloids, Flavanoids, Saponins, Tannins & Resins. The antimicrobial activity of the crude extract from the leaves of various medicinal plants are supposed to be due to the presence of various phytochemical constitutes present in them. Column chromatography is used to purify individual chemical compounds from mixtures of compounds. This also prevents cross-contamination and stationary phase degradation due to recycling. This method was used on chloroform extract for the leaf extract of Erythrina variegata using standard technique. The antibacterial sensitivity potential was assess and recorded on 14 fractions ranging from 1- 14 and compared with Negative control as ?Chloroform? and Positive control as ?Amikacin? treatment. The result confirmed high antibacterial potential in Chloroform extract of Erythrina variegata in comparison with other solvents tested such as n- Hexane, Dichloromethane, Ethyl acetate, Methanol and Aqueous. As the purification of fraction increased the antibacterial sensitivity of chloroform extract enhanced too. The pure solvent at fraction 14 was found to be nearby close to drug ?Amikacin? antibacterial sensitivity, indicating the suitability of chloroform fraction derived from leaf extract of Erythrina variegata to be a potential source of development of newer medicine.
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[Preeti Kumari and Chandrawati Kumari. (2017); COLUMN CHROMATOGRAPHY FRACTIONAL ANALYSIS OF ERYTHRINA VARIEGATA L. LEAF EXTRACT FOR ITS ANTIBACTERIAL EFFICACY. Int. J. of Adv. Res. 5 (Aug). 279-286] (ISSN 2320-5407). www.journalijar.com
P.G Department of Biotechnology, A.N. College, Magadh University, Patna
