15Aug 2017

THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY CLIMBER.

  • Doctor of Philosophy in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.
  • Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.
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An efficient protocol is developed for the induction of shoot regeneration from leaf and node derived callus of Pueraria tuberosa on MS medium supplemented with TDZ (Thidiazuron) (2.0mg/l) and IAA (1.0mg/l) after two of subsequent subcultures. This is the first report of its kind in this plant of Pueraria tuberosa. It is belongs to member of Paplionaceae family. The obtained shoots were elongated after four weeks on the same medium and induced roots by transferring the cultures on half strength MS medium supplemented with 3.0 mg/l IBA after four weeks of inoculation. These plantlets were allowed for hardening and acclimatization for field transfer.

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[V. Sadguna and Md. Mustafa. (2017); THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY CLIMBER. Int. J. of Adv. Res. 5 (Aug). 743-748] (ISSN 2320-5407). www.journalijar.com

Dr.Md.Mustafa
Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.

DOI:

Article DOI: 10.21474/IJAR01/5114      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5114

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