15Aug 2017

THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY CLIMBER.

  • Doctor of Philosophy in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.
  • Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.
  • Abstract
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  • Corresponding Author

An efficient protocol is developed for the induction of shoot regeneration from leaf and node derived callus of Pueraria tuberosa on MS medium supplemented with TDZ (Thidiazuron) (2.0mg/l) and IAA (1.0mg/l) after two of subsequent subcultures. This is the first report of its kind in this plant of Pueraria tuberosa. It is belongs to member of Paplionaceae family. The obtained shoots were elongated after four weeks on the same medium and induced roots by transferring the cultures on half strength MS medium supplemented with 3.0 mg/l IBA after four weeks of inoculation. These plantlets were allowed for hardening and acclimatization for field transfer.


  1. Whyte R.O., Nilsson-Leissner G and Trumble H.C, (1953). Legumes in Agriculture. FAO Agricultural Studies 21, Rome, 367 p.
  2. Skerman P.J, (1977). Tropical Florage Legumes. FAO Plant Production and Protection Series no. 2, Rome.
  3. Duck J.A, (1981). Handbook of legumes of world economic importance plenum Press, New York.
  4. Kashyap M.K., V. Yadav, B.S. Sherawat S. Jain and S. Kumariet al., (2005).?Different antioxidants status, total antioxidant power and free radicals in essential hypertension. Cell Biochem. 277: 89-99.
  5. Kusano C and B. Ferrari, (2008).Total antioxidant capacity: A biomarker in biomedical and nutritional studies. Cell Mol. Biol., 7: 1-15.
  6. Pandey N., J.K. Chaurasia, O.P. Tiwari and Y.B. Tripathi, (2007).Antioxidant properties of different fractions of tubers from?Pueraria tuberosa? Food Chem., 105: 219-222.
  7. Bingham C., Beaman M., Nicholls AJ., Anthony PP, (1998). Necrotizing renal vasculopathy resulting in chronic renal failure after ingestion of methamphetamine and 3, 4-methylenedioxy-methamphetamine. Nephrology Dialysis Transplantation 13:2654-2655.
  8. Khare CP (2007). Indian medicinal plants: An illustrated dictionary. 1st New York: Springer Science; p.527
  9. Mamun A. N. K., Matin M. N., Bari M. A., Sidique N.A., Sultana R. S., Rahman M. H. & Musa A. S. M, (2004).Micropropagation of Woody Legume (Albizia lebbeck) Through Tissue Culture. Pakistan Journal of Biological Sciences. 7 (7):109-103.
  10. Veltcheva M.R., Svetleva D.L, (2005). In vitro regeneration of Phaseolus vulgaris via organogenesis from petiole explants. J Central European Agric 6:53?58.
  11. Priyanka Pandey, Rakesh Mehta and Ravi Upadhyay, (2013). Effect of Explants Type and Different Plant Growth Regulators on Callus Induction and Plantlet Regeneration in Psoralea corylifolia [L]. I. J. R in Pharmaceutical and Biomedical Sciences. Vol. 4(3): 914-918.
  12. Udomasuk L., Jarukamjorn K., Tanaka H and Putalum W, (2009). Production of isoflavonoids in Callus Cultures of Pueraria candollei var. mirifica, 64c, 239-243.
  13. Varisai Mohamed, Shamsudeen; Sung, Jih-Min; Jeng, Toong-Long and Wang, Chang-Sheng, (2006). Organogenesis of Phaseolus angularis L.: high efficiency of advaentitious shoot regeneration from etiolated seedlings in the presence of N6-benzylaminopurine and thidiazuron. Plant Cell Tissue and Organ Culture, August, vol.86, no.2, p.187-199.
  14. Tzitzikas E.N.,?Bergervoet M.,?Raemakers K.,?Vincken J.P.,?Lammeren A and?Visser R.G.F (2004). Regeneration of pea (Pisum?sativum?) by a cyclic?organogenic?system. Plant Cell Reports23: 453-461.
  15. Shinde A.N., Malpathak N., Fulzele D.P, (2009). Induced high frequency shoot regeneration and enhanced isoflavones production in Psoralea corylifolia, Rec Nat Prod., 3:38-45.
  16. K, Lakshmiprabha.A and Jayabalan.N, (2006). Amino acids enhancing plant regeneration from cotyledon and embryonal axis of peanut (Arachis hypogaea L.). Indian J. Crop Science, 1(1-2): 79-83.
  17. Ugandhar T., Venkateshwarlu M., Shekhar G.P.V and Jagan Mohan Reddy K, (2012). High Frequency Somatic Embryogenesis and Plantlet Regeneration from Cotyledon Explants of Pigeon Pea (Cajanus Cajan (L.), A Grain Legume. International Journal of Pharma and Bio Sciences, 3(1), 55-63.
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[V. Sadguna and Md. Mustafa. (2017); THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY CLIMBER. Int. J. of Adv. Res. 5 (Aug). 743-748] (ISSN 2320-5407). www.journalijar.com


Dr.Md.Mustafa
Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.

DOI:


Article DOI: 10.21474/IJAR01/5114      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5114