22Aug 2017

MOLECULAR CHARACTERIZATION OF CELLULASE PRODUCING MICROBACTERIUM BARKERI ISOLATED FROM AGRICULTURAL WASTE.

  • Department of Botany, T.P.S College, Magadh University, Bihar India.
  • Department of Botany, BRABU, Muzaffarpur, Bihar, India.
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The present investigation was undertaken to isolate and screen an efficient cellulase producing bacteria from agricultural waste. Bacterial cultures were isolated from agricultural wastes such as groundnut shell, coconut coir, vegetable peel etc. A total of 28 bacterial cultures were isolated on nutrient agar. The isolates were screened for cellulase production by plating on CMC and Congo red agar media to observe the zone of cellulose hydrolysis. Among all 28 tested bacterial strains, 12 strains showed cellulase production on CMC and congo red agar. Further these 12 isolates were used for crude enzyme production. The carboxy methyl cellulase (CMCase) activity was assayed using the method described by Miller, with some modifications. S7 bacterial isolate which showed maximum enzyme activity (0.430 IU/ml/min) was further subjected to biochemical and molecular characterization. On the basis of 16s rRNA molecular characterization, the isolate S7 was identified as Microbacterium barkeri. Therefore, the cellulase producing Microbacterium sp. isolated and identified in this study have prospective for further use and study, such as to isolate efficient or novel cellulases with unique characteristics and various applications.

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[Tanuja and Jaya Philip. (2017); MOLECULAR CHARACTERIZATION OF CELLULASE PRODUCING MICROBACTERIUM BARKERI ISOLATED FROM AGRICULTURAL WASTE. Int. J. of Adv. Res. 5 (Aug). 1344-1349] (ISSN 2320-5407). www.journalijar.com

Jaya Philip
Department of Botany, BRABU, Muzaffarpur,Bihar, India

DOI:

Article DOI: 10.21474/IJAR01/5188      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5188

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