25Aug 2017

THE UTILITY OF GOLD NANOPARTICLES AS AN EFFECTIVE DRUG CARRIER AND ITS ANTIBACTERIAL EFFICACY WITH GEMIFLOXACIN HCL.

  • Pharmaceutical Chemistry Dept., National Organization for Drug Control and Research [NODCAR], 6 Abu
  • Hazem Street, Pyramids Ave, P.O. Box 29, Giza, Egypt.
  • Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, P.C.44 519, Zagazig, Egypt.
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A simple and extractive spectrophotometric method was developed for the determination of GemifloxacinHCl (GEM) in bulk drug and pharmaceutical formulation. The method depends on the reaction between Gemifloxacin and gold nanoparticles (AuNps). The apparent molar absorptivity and Sandell?s sensitivity were found to be 0.1 x 104 L mol−1 cm−1 and 0.0038 μg cm-2 respectively. Beer?s law was conformed in the concentration range of 1.5 ?38.8μg mL-1. The method is very simple and not time consuming, therefore it can be properly applied for the evaluation of Gemifloxacin in dosage forms and also in vitro antibacterial activities of GEM, gold nanoparticles and drug coated gold nanoparticles against several strains of Gram positive and Gram negative organisms. The antibacterial properties of GEM-AuNps complex were higher than that of the pure Gemifloxacin while gold nanoparticles did not produce any antibacterial activity against the bacterial strains. These results recommend that gold nanoparticles could act as an effective drug carrier in drug delivery system.

  1. Krishna MV, Sankar DG: Utility of σ and π-acceptors for the spectrophotometric determination of gemifloxacin mesylate in pharmaceutical formulations. Journal of Chemistry 2008, 5(3):493-498.
  2. Lowe MN, Lamb HM: Gemifloxacin. Drugs 2000, 59(5):1137-1147.
  3. Lomaestro BM: Gemifloxacin: A broad-spectrum oral quinolone for treatment of respiratory and urinary tract infections. Formulary 2000, 35(12):961-961.
  4. Doyle E, Fowles S, McDonnell D, McCarthy R, White S: Rapid determination of gemifloxacin in human plasma by high-performance liquid chromatography?tandem mass spectrometry. Journal of Chromatography B: Biomedical Sciences and Applications 2000, 746(2):191-198.
  5. Rote A, Pingle S: Reverse phase-HPLC and HPTLC methods for determination of gemifloxacin mesylate in human plasma. Journal of Chromatography B 2009, 877(29):3719-3723.
  6. Cho SI, Shim J, Kim M-S, Kim Y-K, Chung DS: On-line sample cleanup and chiral separation of gemifloxacin in a urinary solution using chiral crown ether as a chiral selector in microchip electrophoresis. Journal of Chromatography A 2004, 1055(1):241-245.
  7. Vinodhini C, Chitras K, Annie A, Marbaniang I, Singh A, Ashok D, Reddy C: Determination of gemifloxacin in tablets by reverse phase high performance liquid chromatography. Indian Drugs 2009, 46:71-73.
  8. Krishna MV, Sankar DG: Spectrophotometric determination of gemifloxacin mesylate in pharmaceutical formulations through ion-pair complex formation. Journal of Chemistry 2008, 5(3):515-520.
  9. Chen Y-H, Tsai C-Y, Huang P-Y, Chang M-Y, Cheng P-C, Chou C-H, Chen D-H, Wang C-R, Shiau A-L, Wu C-L: Methotrexate conjugated to gold nanoparticles inhibits tumor growth in a syngeneic lung tumor model. Molecular pharmaceutics 2007, 4(5):713-722.
  10. Jalil R, Nixon J: Biodegradable poly (lactic acid) and poly (lactide-co-glycolide) microcapsules: problems associated with preparative techniques and release properties. Journal of microencapsulation 1990, 7(3):297-325.
  11. Arulkumar S, Sabesan M: Biosynthesis and characterization of gold nanoparticle using antiparkinsonian drug Mucuna pruriens plant extract. Int J Res Pharm Sci 2010, 4:417-420.
  12. Atta NF, Galal A, Azab SM: Gold Nanoparticles Modified Electrode for the Determination of an Antihypertensive Drug. Electroanalysis 2012, 24(6):1431-1440.
  13. Salama NN, Zaazaa HE, Azab SM, Atty SA, El-Kosy NM, Salem MY: Utility of gold nanoparticles/silica modified electrode for rapid selective determination of mebeverine in micellar medium: comparative discussion and application in human serum. Ionics 2016, 22(6):957-966.
  14. Atta NF, Galal A, Azab SM: Novel sensor based on carbon paste/Nafion? modified with gold nanoparticles for the determination of glutathione. Analytical and bioanalytical chemistry 2012, 404(6-7):1661-1672.
  15. Atta NF, Galal A, Azab SM: Electrochemical morphine sensing using gold nanoparticles modified carbon paste electrode. Int J Electrochem Sci 2011, 6(10):5066-5081.
  16. Brust M, Schiffrin DJ, Bethell D, Kiely CJ: Novel gold‐dithiol nano‐networks with non‐metallic electronic properties. Advanced materials 1995, 7(9):795-797.
  17. Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ: A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 1996, 382(6592):607.
  18. Demers LM, Mirkin CA, Mucic RC, Reynolds RA, Letsinger RL, Elghanian R, Viswanadham G: A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Analytical chemistry 2000, 72(22):5535-5541.
  19. Kim Y, Johnson RC, Hupp JT: Gold nanoparticle-based sensing of ?spectroscopically silent? heavy metal ions. Nano Letters 2001, 1(4):165-167.
  20. Selvaraj V, Alagar M, Hamerton I: Analytical detection and biological assay of antileukemic drug using gold nanoparticles. Electrochimica Acta 2006, 52(3):1152-1160.
  21. Sharada C, Channabasavaraj K, Mani TT: Development of a spectrophotometric method for the quantitative estimation of zidovudine concentration in bulk and pharmaceutical dosage forms. KMITL Science and Technology Journal 2014, 10(1).
  22. Menon SK, Mistry BR, Joshi KV, Sutariya PG, Patel RV: Analytical detection and method development of anticancer drug Gemcitabine HCl using gold nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2012, 94:235-242.
  23. Selvaraj V, Alagar M: Analytical detection and biological assay of antileukemic drug 5-fluorouracil using gold nanoparticles as probe. International journal of pharmaceutics 2007, 337(1):275-281.
  24. Job P: Formation and stability of inorganic complexes in solution. 1928.
  25. Sommer L, Havel J, Kub?ň V: Spectrophotometric studies of the complexation in solution: Univerzita JE Purkyně; 1970.
  26. Atta NF, Galal A, Azab SM, Ibrahim AH: Electrochemical Sensor Based on Ionic Liquid Crystal Modified Carbon Paste Electrode in Presence of Surface Active Agents for Enoxacin Antibacterial Drug. Journal of The Electrochemical Society 2015, 162(1):B9-B15.
  27. Motwani SK, Chopra S, Ahmad FJ, Khar RK: Validated spectrophotometric methods for the estimation of moxifloxacin in bulk and pharmaceutical formulations. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2007, 68(2):250-256.
  28. Raut C, Gharge D, Dhabale P, Gonjari I, Hosmanib A, Hosmani A: Development and Validation of Oseltamivir Phosphate in Fluvir? by UV spectrophotometer. International Journal of PharmTech Research 2010, 2(1):363.
  29. Charan DC, Satyabrata S: Simple and rapid spectrophotometric estimation of gemifloxacin mesylate in bulk and tablet formulations. International Journal of PharmTech Research 2011, 3(1):133-135.

[Amira S. Eldin, *Shereen M. Azab, Abdallah A. Shalaby andMagda El-Maamly. (2017); THE UTILITY OF GOLD NANOPARTICLES AS AN EFFECTIVE DRUG CARRIER AND ITS ANTIBACTERIAL EFFICACY WITH GEMIFLOXACIN HCL. Int. J. of Adv. Res. 5 (Aug). 1649-1658] (ISSN 2320-5407). www.journalijar.com

Shereen M. Azab
National Organization for Drug Control and Research

DOI:

Article DOI: 10.21474/IJAR01/5226      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5226

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