Vol. 5 (10) pp. 478-487 DOI: 10.21474/IJAR01/5552

IMMUNOHISTOCHEMICAL AND MORPHOMETRICAL STUDY OF GESTATIONAL INDUCED CHANGES IN MOUSE LIVER USING ANTI-ADIPONECTIN RECEPTOR 2.

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  • Department of Human Anatomy, Section of Histology& Embryology, University of AL-Nahrain, College of Medicine, Baghdad, Iraq.
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Abstract

The liver is one of accessory organs associated with the digestive tract, it plays major role in carbohydrate and protein metabolism, inactivates many toxic substances and drugs, and synthesizes most plasma proteins and factors necessary for blood coagulation. The liver parenchyma represented by hepatocytes is adapted with the body changes in pregnancy. This study aimed to analyze the histomorphometrical changes in liver tissue and their cells in late pregnancy with special emphasis on the immunohistochemical reactivity of hepatocyte and their adaptation toward pregnancy changes using anti adiponectin receptor 2. The study design enrolled two groups; pregnant mice (15) in late gestational period and non pregnant mice (15); the liver tissues were collected and processed for paraffin block then sectioned, anti Adiponectin receptor 2 Ab was used to demonstrate hepatocyte cells reactivity; quantitification of adiponectin receptor2 Ab reactivity done using Aperio Alogrithm soft ware. The present study was designed to assess the changes in the liver in different aspects. General morphological result showed changes of liver include increase in total body weight. Statistical analysis of the morphological changes recorded showed that the weight 1.611 ? 0.061gram in control group and 2.255 ? 0.043gram of the pregnant group and the staining reactivity of anti- Adiponectin receptor 2 Ab in cells of liver in control group show high difference in positivity the mean was (0.781 ? 0.031pixel/micron), while weakly positive in pregnant group with mean (0.180 ? 0.006 pixel/micron). The study concluded that liver tissue adiponectin reactivity changes significantly with pregnancy.

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How to Cite This Article

Zahraa Fawzi A. Mohammed and May Fadhil AL-Habib. (2017); IMMUNOHISTOCHEMICAL AND MORPHOMETRICAL STUDY OF GESTATIONAL INDUCED CHANGES IN MOUSE LIVER USING ANTI-ADIPONECTIN RECEPTOR 2., Int. J. of Adv. Res., 5 (10), 478-487, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5552

Corresponding Author

May Fadil Majed
College of medicine/ Department of histology and emberyology
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.