Vol. 5 (10) pp. 684-694 DOI: 10.21474/IJAR01/5576

FIBRONECTIN EXPRESSION PATTERN IN SKELETAL MUSCLE REGENERATION.

and
  • Department of histology & Embryology, University of AL- Nahrian, Baghdad, Iraq.
  • Department of histology & Embryology, University AL- Nahrian, Baghdad, Iraq.
42 Downloads 186 Views
Crossref

Download Full Paper (PDF)

Abstract

Skeletal muscle is the largest component in the humane bodies, the main function of skeletal muscle is drive locomotion. Muscle fibers composed of myofibrils, the myofibrils are composed of actin and myosin filaments interact to from actinomyosin cross bridge, fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins .Similar to integrins, fibronectin binds extracellular matrix components such as collagen, fibrin, and heparin sulfate proteoglycans. The present study enrolled 40 mature male rabbits. The Extensor digitorum longus muscle was set as amodel for skeletal injury, extensor digitorum longus muscle taken after 3&6 weeks post operatively. Muscle tissues were histologically prepared for paraffin section a proceeded with immunohistochemical reaction fibronectin Ab. The Aperio software for quantitative immunohistochemical reaction was used. Fibronectin antibody was used in this study to asses it is role and changes during the process of regeneration there was a significant difference between the 3rd week's groups and other 2 groups (6th weeks and control groups). This study occluded that there is an important role for fibronectin in skeletal muscle regeneration

Keywords

Article Analytics

References

  1. Hung, C., Linn, G., Chow, Y. H., Kobayashi, A., Mittelsteadt, K., Altemeier, W. A. & Duffield, J. S. (2013). Role of lung pericytes and resident fibroblasts in the pathogenesis of pulmonary fibrosis.?American journal of respiratory and critical care medicine,?188(7), 820-830.‏
  2. Tedesco, F. S., Dellavalle, A., Diaz-Manera, J., Messina, G., &Cossu, G. (2010). Repairing skeletal muscle: regenerative potential of skeletal muscle stem cells.?The Journal of clinical investigation,?120(1), 11.‏
  3. Musar?, A. (2014). The basis of muscle regeneration.?Advances in Biology,?2014.‏
  4. ‏Lehto, M., Duance, V. C., &Restall, D. (1985). Collagen and fibronectin in a healing skeletal muscle injury. An immunohistological study of the effects of physical activity on the repair of injured gastrocnemius muscle in the rat.?Bone & Joint Journal,?67(5), 820-828.‏
  5. Huijbregts, J., White, J. D., & Grounds, M. D. (2001). The absence of MyoD in regenerating skeletal muscle affects the expression pattern of basement membrane, interstitial matrix and integrin molecules that is consistent with delayed myotube formation.?Actahistochemica,?103(4), 379-396.‏
  6. Gulati, A. K., Reddi, A. H., &Zalewski, A. A. (1983). Changes in the basement membrane zone components during skeletal muscle fiber degeneration and regeneration.?The Journal of cell biology,?97(4), 957-962.‏
  7. Singh, P., Carraher, C., &Schwarzbauer, J. E. (2010). Assembly of fibronectin extracellular matrix.?Annual review of cell and developmental biology,?26, 397-419.‏
  8. Dessau, W., Von Der Mark, H., Von Der Mark, K., & Fischer, S. (1980). Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis.?Development,?57(1), 51-60.‏
  9. Gulati, A. K., Reddi, A. H., &Zalewski, A. A. (1982). Distribution of fibronectin in normal and regenerating skeletal muscle.?The Anatomical Record,?204(3), 175-183.‏
  10. To, W. S., &Midwood, K. S. (2011). Plasma and cellular fibronectin: distinct and independent functions during tissue repair.?Fibrogenesis& tissue repair,?4(1), 21.‏
  11. Sottile, J., & Hocking, D. C. (2002). Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions.?Molecular biology of the cell,?13(10), 3546-3559.‏
  12. Sottile, J., Hocking, D. C., &Swiatek, P. J. (1998). Fibronectin matrix assembly enhances adhesion-dependent cell growth.?Journal of cell science,?111(19), 2933-2943.‏
  13. Lovering, R. M., & De Deyne, P. G. (2004). Contractile function, sarcolemma integrity, and the loss of dystrophin after skeletal muscle eccentric contraction-induced injury.?American Journal of Physiology-Cell Physiology,?286(2), C230-C238.‏

How to Cite This Article

Taghreed Abdul Rasool Ali and May Fadhil Al- Habib. (2017); FIBRONECTIN EXPRESSION PATTERN IN SKELETAL MUSCLE REGENERATION., Int. J. of Adv. Res., 5 (10), 684-694, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5576

Corresponding Author

May Fadil Majed
College of medicine/ Al-Nahrain University
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.