29Oct 2017

EFFECT OF UNDER CONVENTIONAL SOLVENT EXTRACTION ON POLYPHENOL CONCENTRATIONS AND ANTIOXIDANT ACTIVITIES IN Thespesia lampas.

  • School of Environment and Sustainable Development, Central University of Gujarat, Sector -30, Gandhinagar, 382030, Gujarat, India.
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Objectives: This study aimed to evaluate the polyphenol content and antioxidant activity in dried leaf, fruit, root and flower of Thespesia lampas Dalz. and Gibs (Cav.) extracted in acetone, isopropanol, acetonitrile and dicholoromethane (under conventional solvents). Material and methods: The antioxidant profile was evaluated using total antioxidant capacity (TAC), ABTS and DPPH assay, Fe2+ chelating ability, iron reducing power (RP) in vitro assays. Results: Highest total phenolic content (TPC) and flavonoid were noted in acetone extracted flower (Fac) and dichloromethane extracted root (Rdcm) respectively. Fac showed maximal activity for TAC (212.16 ?M TE/ mg DW), ABTS (IC50 = 1.12 ? 0.00 mgmL-1), DPPH (IC50 = 0.87 ? 0.01 mgmL-1) and RP (IC50 = 67.48 ? 0.54 mgmL-1) while Rdcm showed highest Fe2+ chelating ability (IC50 = 1.70 ? 0.05 mgmL-1). Acetone emerged as the most efficient solvent in extraction of antioxidants among the solvents exercised. A positive correlation was observed between TPC and ABTS (R2 = 0.952), TPC and TAC (R2 = 0.881), ABTS and TAC (R2 = 0.892); and DPPH and RP (R2 = 0.688) assays. Conclusions: Efficiency of under conventional solvents for extraction of potent organs of Thespesia lampas leading to higher antioxidant activity as a promising source.

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[Sunayana Nath and Bhawana Pathak. (2017); EFFECT OF UNDER CONVENTIONAL SOLVENT EXTRACTION ON POLYPHENOL CONCENTRATIONS AND ANTIOXIDANT ACTIVITIES IN Thespesia lampas. Int. J. of Adv. Res. 5 (Oct). 1749-1757] (ISSN 2320-5407). www.journalijar.com

Bhawana Pathak
School of Environment and Sustainable Development, Central University of Gujarat, Sector -30, Gandhinagar, 382030, Gujarat, India.

DOI:

Article DOI: 10.21474/IJAR01/5698      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5698

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