Vol. 5 (10) pp. 1789-1795 DOI: 10.21474/IJAR01/5702

HEAVY METAL BIOSORPTION POTENTIAL OF PSEUDOMONAS SP. ISOLATED FROM INDUSTRIAL WASTEWATER OF HARIDWAR DISTRICT, INDIA.

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Abstract

The present study was done to evaluate the heavy metal biosorption potential of two bacterial strains isolated from industrial wastewater of Haridwar industrial area. The wastewater samples were collected from four different sites., i.e., HI, BI, OD and DI. The heavy metal concentrations were determined after digestion of wastewater samples. The heavy metal resistant bacteria were isolated and screened for their Biosorption potential. The minimum inhibitory concentration (MIC) of Pb, Cr, Ni and Zn was determined by agar diffusion method in 25, 50, 100, 200 & 400 ppm concentrations. The isolates were identified and characterized molecularity with 16S rRNA gene sequence analysis and were revealed 99% similarities of BIN and BOD with Pseudomonas geniculate and Pseudomonas hibiscocola respectively. Biosorption experiments indicate that, BIN and BOD strain could bioadsorb metals in the order Pb>Cr>Zn>Ni and Ni>Cr>Pb>Zn.

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References

  1. Mulligan, C. N., Yong, R. N. and Gibbs, B. F. (2001). Remediation technologies for metal-contaminated soil and groundwater: an evaluation. Engin. Geol, 60: 193-207.
  2. Anderson, D. (2003). Introduction to heavy metal monitoring, European Environment Agency (EPA), environment assessment report no. 10, Europe?s environmental :- the 3rdassessment,published on web by Epa.
  3. Shayley H., Mcbride M, and Harrison E. (2009). Sources and impacts of contamination in soils. Cornell waste management Institute. 1-6.
  4. Fostner, U. and Wittmann, GTW (1983). Metal pollution in the aquatic environment Gremany: Springer- Verlag Berlin Heidelberg.
  5. - Hussien, H., Farag, S. and Moawad, H. (2003). Isolation and characterization of Pseudomonas resistant to heavy metals contaminants. Arab Journal of Biotechnology. 7: 13-22.
  6. Volesky, B., 1990. Biosorption of Heavy metals. Biotechnology Progress. 11, 235-250.
  7. Analya, N., Ramachandra, T. V., KANAmadi, R. D., (2003). Biosorption of Heavy metals. Journal of Chem. Env.7, 4:71-70. ISSN 0972-0626.
  8. APHA
  9. Aneja, K. R.(2010).In: Experiments in microbiology, plant pathology and biotechnology (4th edition ). New age international (pvt) ltd., New Delhi.
  10. Hansson, P.J.; Edwards, N.T. and Andrews, J.A. (2003).Effect of different tree species on soil properties.Journal of Applied Ecology.23: 657-666.
  11. Sam brook J, Fritsch EF, ManiatisT(1989) Molecular cloning: a laboratory manual (2nd edn), cold spring harbour, New York.
  12. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., and Kumar S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.
  13. Puranik, P. R. and Paknikar, K. M. (1999): Biosorption of lead, cadmium and zinc by Citrobacter strain MCM B-181: characterization studies. Biotechnol. Prog., 15: 228-237.
  14. Cybulski Z, Dzuirla E, Kaczorek E, Olszanowski A, (2003). The influence of emulsifiers on hydrocarbon biodegradation by pseudomonadacea and Bacillacea strains. Spill Science and technology bulletin 8:503-507
  15. John G H; Noel R K; Peter H A S; James T S and Stanley T W.(1994) Bergey‟s manual of Determinative Bacteriology (9th edition). Lippincott Williams & Wilkins, New York.

How to Cite This Article

Namitajoshi, Deep gupta and Anubala. (2017); HEAVY METAL BIOSORPTION POTENTIAL OF PSEUDOMONAS SP. ISOLATED FROM INDUSTRIAL WASTEWATER OF HARIDWAR DISTRICT, INDIA., Int. J. of Adv. Res., 5 (10), 1789-1795, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/5702

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Anu bala
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