10Nov 2017

GENETIC CHARACERIZATION OF NDV STRAINS ISOLATED FROM BROILER CHICKENS OUTBREAKS IN EGYPT.

  • Microbiology and Immunology Department, Veterinary Research Division, National Research Center.
  • Virology Department, Faculty Of Veterinary Medicine, Cairo University.
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In the present study, NDV strains isolated from outbreaks in broiler farms in Egypt were molecularly characterized in 2009. Field samples were collected from different 50 broiler flocks distributed in Egyptian governorates showing characteristic NDV signs and post mortum lesions. Nested PCR was carried out by application of RT-PCR for amplification of regions of genes encoded for the fusion and matrix proteins followed by second PCR, the second PCR products (about 300bps) were sequenced for characterization of the virus. MEGA program version 4 was used for Phylogenetic analysis of sequenced NDV local isolates as well as ten selected known global (I-X) NDV genotypes. The results revealed that those isolates were related to genotype II (Lasota like strain) (Accession No. AF077761) while one isolate (NDV Giza VRLCU 09 23) was related to APMV1-Ch-Shandong-SRZ-03 strain (Accession No. DQ417112). The occurrence of breaks in vaccinated flocks suggests that massive vaccination with live vaccines and use of more than one type of vaccine leads to evolving of new field lentogenic strains which may be classified as new genotypes due to mutations leading to increase outbreaks and the newly strains are conflicted with virus field infection.

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[EL-Dabae. W. H., Hussein. H. A, Ata. Nagwa. S. and Reda. I. M. (2017); GENETIC CHARACERIZATION OF NDV STRAINS ISOLATED FROM BROILER CHICKENS OUTBREAKS IN EGYPT. Int. J. of Adv. Res. 5 (Nov). 300-309] (ISSN 2320-5407). www.journalijar.com

EL-Dabae, W.H
Microbiology and Immunology Department, Veterinary Research Division, National Research Center.

DOI:

Article DOI: 10.21474/IJAR01/5771      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5771

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