29Nov 2017


  • Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 BP 2009 Cotonou, R. Benin.
  • Laboratory of Experimental and Clinic Biology (LaBEC), Faculty of Sciences and Techniques of Dassa, National University of Sciences, Technologies, Engineering and Mathematics (UNSTIM), R. Benin, BP 14 Dassa-Zoum?.
  • Laboratoire d\'Etude et de Recherche en Chimie Appliqu?e (LERCA); Ecole Polytechnique d\'Abomey-Calavi / Universit? d\'Abomey-Calavi (UAC), R. B?nin.
  • Human Biology Unit, Faculty of Health Science, 01 B.P. 188 Cotonou, R. Benin.
  • Laboratoire de Physiologie/Pharmacologie, Facult? des Sciences, Universit? de Lom?, Togo.
  • Laboratory of Biomembranes and Cellular Signaling, University of Abomey-Calavi 01 BP 188 Cotonou Benin.
  • Laboratoire de Biologie et de Typage Mol?culaire en Microbiologie, University of Abomey-Calavi 01 BP 188 Cotonou Benin.
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Cocos nucifera is a tropical coastal plant. The ethyl acetate fraction of its aqueous root extract stimulates erythropoiesis. This work aimed to evaluate the biological tolerance of this fraction. The extract fraction was administered to Wistar rats at a single dose of 2000 mg / kg body weight for Acute Oral Toxicity (OAT) or in daily doses of 200 mg / kg body weight for 28 days for Sub-Chronic Toxicity. (SCT), as recommended by the OECD. On day 0, then on day 14 for OAT and day 28 for SCT, the rats were weighed and their blood was collected for tests. The liver balance was assessed by measuring transaminases AST and ALT, kidney balance by uremia and creatinine level and immune status by blood leukocyte count. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. The weight of rats, the balance of liver, kidney and immunity as well as these organs histology were not affected by either acute oral or sub-chronic toxicity tests, suggesting a lack of toxicity in the extract fraction. The ethyl acetate fraction of the root water extract of Cocos nucifera did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids that are antioxidants. The study of biological tolerance deserves to be continued by the chronic toxicity test and clinical trials.

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[Maximin Senou, Atchade Pascal Tchogou, T.Victorien Dougnon, Cokou Pascal Agbangnan Dossa, Pidox Ogue, Angelique Agossadou, Anatole Laleye, Frederic Loko, Amegnona Agbonon, Alphonse Sezan and Lamine Babamoussa. (2017); SAFETY OF ETHYL ACETATE FRACTION OF COCOS NUCIFERA ROOT EXTRACT. Int. J. of Adv. Res. 5 (Nov). 1083-1090] (ISSN 2320-5407). www.journalijar.com

Maximin SENOU
Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 BP 2009 Cotonou, R. Benin


Article DOI: 10.21474/IJAR01/5869      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5869