30Nov 2017

IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF CHLORELLA VULGARIS.

  • Department of Biochemistry, Mohamed Sathak College of Arts & Science, Sholinganallur, Chennai, Tamil Nadu, India.
  • Department of Biotechnology, Mohamed Sathak College of Arts & Science, Sholinganallur, Chennai, Tamil Nadu, India.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The present study focuses on the quantification of total phenolics and flavonoids using Gallic acid and Rutin as standard respectively. The antioxidant activity of methanolic extract of Chlorella vulgaris was also determined. The antioxidant and free radical scavenging potential was due to the high content of phenolics [0.022 ? 0.015 μg/ml] and flavonoids [0.054 ? 0.031 μg/ml]. The microalga extracts with good antioxidant capacities can be explored for new natural antioxidant sources.

  1. Annamalai, J. and Nallamuthu, T. (2014): Antioxidant potential phytochemicals from methanol extract of Chlorella vulgaris and Chlamydomonasreinhardtii. Journal of Algal Biomass Utilization.,5: 60-67.
  2. Badmus, J.A.,Odunola,O.A.,Yekeen,T.A.,Gbadegesin,A.M.,Fatoki,J.O., Godo, M.O., Oyebanjo,K.S. and Hiss,D.C. (2013): Evaluation of antioxidant, antimutagenic, and lipid peroxidation inhibitory activities of selected fractions of Holarrhena floribunda(G. Don) leaves. ACTA Biochimica Polonica.,60: 435-442.
  3. Chang, C .C., Yang,M.H., Wen,H.M. and Chern,J.C.(2002):Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis.10: 178-182.
  4. Dantas, D.M.M., Costa,R.M.P.B., Carneiro-da-Cunha,M .G., Galvez,A.O., Drummond,A.R. and Bezerra,R.S.(2015): Bioproduction, Antimicrobial and Antioxidant Activities of Compounds from Chlorella vulgaris. Research &Reviews.Journal of Botanical Sciences.4: 12-18.
  5. Fadzai Boora, Elaine Chirisa. andStanley Mukanganyama. (2014): Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. Journal of Food Processing., 2014: 1-7.
  6. Goiris, K., De Vreese, P., De Cooman, L., Muylaert, K.(2012): Rapid screening and? guided? extraction? of? antioxidants? from microalgae using voltammetric methods. J Agric Food Chem.,60:7359-66
  7. Goiris, K., Muylaert, K., Fraeye, I., Foubert, I., Brabanter, J. D. and Cooman, L. D. (2012): Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. J Appl Phycol.,24: 1-10.
  8. Herrero, M., Mart?n-?lvarez, P.J., Senorans, F.J., Cifuentes, A., and Ib??ez, E. (2005). Optimization of accelerated solvent extraction of antioxidants from Spirulinaplatensis microalga. Food Chemistry.,93:417-423.
  9. Indu, H and Seenivasan, R.(2013): In VitroAntioxidant Activity of Selected Seaweeds from Southeast Coast of India. International Journal of Pharmacy and Pharmaceutical Sciences.,5: 474-484.
  10. Kuda ,T., Tsunekawa, M., Hishi, T., Araki, Y. (2005): Antioxidant properties of dried? kayamo-nori, a brown alga Scytosiphonlomentaria (Scytosiphonales, Phaeophyceae. Food Chem.,89: 617-622.
  11. Lee, S.H., Lee, J.B., Lee, K.W. and Jeon, Y.J.(2010): Antioxidant Property? of Tidal Pool Microalgae,Halochlorococcumporphyrae and Oltamannsiellopsisunicellularis From Jeju Island, Korea?ALGAE
  12. Manivannan, K., Anantharaman, P., Balasubramanian, T.(2012): Evaluation of antioxidant properties of marine microalga Chlorella marina (Butcher, 1952). S342-S346.
  13. Natrah, F.M., Yusoff, F.M., Shariff, M., Abbas, F., Mariana, N.S.(2007): Screening of Malaysian indigenousmicroalgae for antioxidant properties and nutritional value. J. Appl. Phycol.,19:711?718
  14. Nick, G.L.(2003): Addressing human exposure to environmental toxins with Chlorella pyrenoidosa - medicinal properties in whole foods. Townsend Letter for Doctors and Patients.,237: 28-32.
  15. Nishimiki, M., Rao,N.A. and Yagi, K.(1972): The occurrence of super-oxide anion in the reaction of reduced phenazinemethosulfate and molecular oxygen. Biochemistry and Biophysical Research and Communications.,46: 849-853.
  16. Oyaizu,M.(1986): Studies on product of browning reaction prepared from glucoseamine. Japanese Journal of Nutrition.,44: 307-315.
  17. Prieto, P., Pineda, M. and Anguilar, M. (1999): Anal.Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex Specific Application to the Determination of Vitamin E. Analytical ,269: 337-341.
  18. Re,R., Pellegrini,N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans,C. (1999): Antioxidant activity applying an improved ABTS radical cation-decolorizing assay. Free Radical Biology and Medicine.,26: 1231-1237.
  19. Ruch,R.J., Cheng,S.J. and Klaunig,J.E.(1989): Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis.,10: 1003.
  20. Shibata,T., Hama,Y., Miyasaki,T., Ito,M., Nakamura,T.J.(2006): Extracellular secretion? of? phenolic? substances? from? living brown algae. ApplPhycol.,18: 787-94.
  21. SivaKumar ,K. and. Rajagopal, S. V.,(2011): Radical scavenging activity of green algal species.?Journal of Pharmacy Research., 4: 723?725.
  22. Suganya,V., Anuradha,V., Syed ali,M., Sangeetha, P. and Bhuvana, P. (2017): In vitro antioxidant activity of microencapsulated and non-encapsulated astaxanthin. Asian Journal of Science and technology; 8: 6391-6404.
  23. Taga, M.S., Miller, E.E and Pratt, D.E. (1984): Chia seeds as a source of natural lipid antioxidants. Journal of American Oil Chemists Society., 61: 928?931.
  24. Uma, R. Sivasubramanian ,V. and? NiranjaliDevaraj S.(2011):?Evaluation of in vitro antioxidant activities and antiproliferative activity of green microalga? Desmococcusolivaceous and? J. Algal Biomass Utln.,2 : 82? 93
  25. Wang, H.M.,Pan, J.L.,Chen,C.Y.,Chiu,C.C.,Yang ,M.H.,Chang, H.W. and Chang, J.S.(2010):Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property using supercritical carbon dioxide extraction. Proc Biochem.,45:1865?1872.
  26. Winterbourn , C.C, Hawkins, R.E., Brian, M. and Carrell ,R.W.(1975): The estimation of red cell superoxide dismutase activity. See comment in PubMed Commons belowJournal of Laboratory Clinical Medicine., 85:337-341.
  27. Yen, G.H. and Chen,H.Y.(1995): Antioxidant Activity of Various Tea Extracts in Relation to their Antimutagenicity. Journal of Agricultural Food Chemistry., 43:27?32.
  28. Zargar, M., Azizah, H., Roheeyati, A. M., Fathimah, A. B., Jahanshiri, F. and Pak-Dek, M. S.(2011) Bioactive compounds and antioxidant activity of different extracts from Vitexnegundo Journal of Medical Plant Research.,5: 2525-2532.

[Bhuvana P, Anuradha. V, Syed Ali.M, Suganya V and Sangeetha P. (2017); IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF CHLORELLA VULGARIS. Int. J. of Adv. Res. 5 (Nov). 1465-1474] (ISSN 2320-5407). www.journalijar.com

Dr.Anuradha.V
Department of Biochemistry, Mohamed Sathak College of Arts and Science

DOI:

Article DOI: 10.21474/IJAR01/5922      
DOI URL: https://dx.doi.org/10.21474/IJAR01/5922

Download Full Paper

Download PDF No. of Downloads: 70 | No. of Views: 212