ANTIBIOTIC ACTION OF PYOCYANIN ON SOME PATHOGENIC BACTERIA ISOLATED FROM WOUND INFECTION.
- Sulaimani Polytechnic University, Technical college of Health, Medical Laboratory Department.
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Abstract
Bacteria Pseudomonas aeruginosa has ability to survive in various niches due to its capacity of production and releasing some compounds, which act as inhibitory effect of bacteria, fungi and protozoa, etc. It has been reported that the synthesis of pyocyanin by many strains could be the reason for P. aeruginosa to survive and compete other microorganisms in the nature. Antibacterial activity of pigment pyocyanin that extracted from P. aerogenosa has been studied using gram negative bacteria Klebsiella oxytoca, Proteus mirabilis, Escherichia coli and gram positive bacteria staphylococcus aureus. At the same time, antibiotic susceptibility test was performed for all bacteria using 12 different antibiotics in the absence and presence of pyocyanin pigment. As a result, all bacteria were sensitive in the presence of pyocyanin, while all bacteria were resistant to used antibiotics without pyocyanin except Proteus mirabilis, which was sensitive to both antibiotics Tetracycline and Amikacin. However, the results of bacterial sensitivity test to antibiotics in the presence of pyocyanin were variable.
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References
- Aonofriesei F and Cr?smaru M. (2002). Antibacterial activity of pyocianin produced by some Pseudomonas strains isolated from seawater. Ovidius University, Faculty of Natural Sciences and Agriculture Sciences, Blvd. Mamaia 124.
- Atlas r.m., brown a.e. and parks l.c. (1995). Laboratory manual experimental microbiology. mosby-yearbook inc., st. Louis.
- Baront Stephen S. and Rowe John J., (1981). Antibiotic Action of Pyocyanin. Antimicrobial agents and chemotherapy. p. 814-820 Vol. 20, No. 6.
- Costa Rita Ana, Batist?o W. F. Deivid, Ribas M. Rosineide, Sousa Margarida Ana, Pereira M. Olivia and Botelho M. Claudia. (2013). Staphylococcus aureus virulence factors and disease. Microbial pathogens and strategies for combating them: science, technology and education (A. M?ndez-Vilas, Ed.).
- Cruick Shank, R;D Duguid; B.Pmarmition and R.H.A. Swain. (1975). Medical microbiology, the practice of medical microbiology. 12thed.,vol.1. Churchill living stone, london and new York.
- El-Fouly M.Z., Sharaf A. M., Shahin A.A.M., El-Bialy H.A., (2015). Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa, J. Radiat. Res. Appl. Sci.,36-48. 8.
- El-Shouny W.A., Al-Baidani A.R.H., Hamza W.T., (2011). Antimicrobial activity of pyocyanin produced by Pseudomonas aeruginosa isolated from surgical wound infections, Int. J. Pharm. Med. Sci.,1-7. 1.
- Groscop, J.A., Brent, M.M. (1990). The effects of selected straines of pigmented microorganisms on small free-living ameobae. Can. J. Microbiol. 10: 579-584.
- L.G., Foster.S.J., and Richards R.G. (2002).An Introduction To Staphylococcus aureus, and Quantifying S. aureus Adhesins In Relation To Adhesion To Biomaterials;Review. European cells and Materials Vol. 4. 39-60.
- Hoadley, A.W., Ajello, G. (1982). Some characteristic of fluorescent pseudomonads isolated from surface waters and capable of growth at 41oC. Can. J. Microbiol. 18: 1769-1773.
- Law, D. (2000).The History and Evolution of Escherichia coli O157 and other Shiga toxinproducing E. coli. 16:701-709.
- Marshall, K.C., Stout, R., Mitchell, R. (1986). Mechanism of the initial events in the sorption of marine bacteria to surfaces. J. Gen. Microbiol.68: 337-348.
- McGowan, R.P., Cheng, K.-L., Bailey, C.B.M., Costerton, J.W. (1988). Adhesion of bacteria to epithelial cell surfaces within reticulo-rumen of cattle. Appl. Environ. Microbiol. 35; 149-155.
- Nester EW; Anderson DG. (2001). A human perspective In Microbiology, 3rd ed, McGraw-Hill companies, New Jersey, USA.
- Norman R.S., Moeller P., McDonald T.J., Morris P.J., (2002). Effect of pyocyanin on a crude-oil degrading microbial community, Appl. Anviron. Microbiol., 4004-4011. 70.
- ?zy?rek Sezen Bilen, G?r Sinem Diken and Bilkay Işıl Seyis. (2016). Investigation of Antimicrobial Activity of Pyocyanin Produced by Pseudomonas aeruginosa Strains Isolated from Different Clinical Specimens. S.B. ?zy?rek et al. / Hacettepe J. Biol. & Chem.,44 (1),1?6.
- Paerl, W. (1989). Microbial attachment to particles in marine amd freshwater ecosystems. Microb. Ecol.2: 73-83.
- Saha S., Thavas R., Jayalakshmi S., (2008). Phenazine pigments from Pseudomonas aeruginosa and their application as antibacterial agent and food colourants, Res. J. ,122-128. 3.
- Schaffer N. Jessica and Melanie M. Pearson. (2015). Proteus mirabilis and Urinary Tract Infections. Microbiol Spectr. 3(5)10.1128.
- Schoental, B. (1952). The nature of the antibacterial agents present in Pseudomonas pyocyanea cultures. Brit. J. Exp. Pathol. 22: 137-147.
- Sohn Kyung Mok, Kang Cheol-In, Joo Eun-Jeong, Ha Young Eun, Doo Ryeon, Chung Kyong Ran Peck, Lee Nam Yong and Song Jae-Hoon. (2011). Epidemiology of Ciprofloxacin Resistance and Its Relationship to Extended-Spectrum β-Lactamase Production in Proteus mirabilis Bacteremia. Original Article. 26.1.89.
- Stock Ingo and Wiedemann. (2001). Natural antibiotic susceptibility of Klebsiella pneumoniae, k. oxytca, K. planticola, K. orinthinolytica and K. terrigena strains. J. Med. Microbiol. Vol. 50, 396-406.
- Sweedan E.G., (2010). Study the effect of antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as antibiotic against different pathogenic bacteria, J. University of anbar for pure science, 4.
- Zinnah M. A., Bari M. R., Islam M. T., Hossain M. T., Rahman M. T., Haque M. H., Babu S. A. M., Ruma R. P. and Islam M. A. (2007). Characterization? Of Escherichia coli Isolated From Samples Of Different Biological And Environmental Sources. Bangl. J. Vet. Med. 5 (1 & 2): 25?32.
How to Cite This Article
Sabiha Sharif salih and Lana M A. Mohammed. (2017); ANTIBIOTIC ACTION OF PYOCYANIN ON SOME PATHOGENIC BACTERIA ISOLATED FROM WOUND INFECTION., Int. J. of Adv. Res., 5 (12), 1197-1201, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/6076
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