04Jan 2018

IDENTIFICATION OF CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA USING CRIPTO-1.

  • Assistant Lecturer, Oral Pathology Department, Faculty of Dentistry, October 6 University, Egypt.
  • Lecturer, Oral Pathology Department, Faculty of Dentistry, October 6 University, Egypt.
Crossref Cited-by Linking logo

  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Background: Oral squamous cell carcinoma (OSCC) is still one of the most common lethal malignancies of the oral cavity. One of the reasons for its treatment failure is thought to be related to the presence of a subpopulation of cells within the tumor called cancer stem cells (CSCs). CSCs display stem cell-like characteristics that impart resistance to conventional treatment modalities and promote tumor initiation, progression, and metastasis. Specific markers for this population have been investigated in the hope of developing a deeper understanding of their role in the pathogenesis of OSCC and elucidating novel therapeutic strategies. Over the past years, different techniques and markers have been investigated. Cripto-1(CR-1) is an identified oncogene which was overexpressed in a wide spectrum of human malignant solid tumours, introducing a potential therapeutic target for human cancers and was significantly associated with initiation and aggressiveness of human cancers. Methods: In this study 40 samples of well and poorly differentiated squamous cell carcinoma were obtained to study the difference of cripto-1 expression according to the degree of differentiation using the immunofluorescent technique. Results: CR-1 was over expressed in well differentiated samples compared to poorly differentiated samples and immunofluorescent expression showed significant decrease in mean percentage (P˂ 0.05). Conclusion: CSCs has a role in initiation and progression of OSCC and CR-1 can be considered as a good marker for detection of these cells.

  1. Al-Hajj, M., Wicha, M., Benito-Hernandez, A., Morrison, S. and Clarke, M. (2003): Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U S A,100:3983-3988.
  2. Baillie, R; Tan, S, Tand Itinteang,T (2017): Cancer Stem Cells in Oral Cavity Squamous Cell Carcinoma: A Review. Frontiers in Oncology, V (7), 112:1-10.
  3. Bianco, C; Rangel, MC; Castro, NP; Nagaoka,T; Rollman, K and Gonzales, M and Salomon, D (2010): Role of Cripto-1 in Stem Cell Maintenance and Malignant Progression. The American Journal of Pathology, Vol. 177, No. 2, 1-9.
  4. Burkert, J; Wright, N.A; Alison, M.R (2006): Stem cells and cancer: an intimat relationship. J Pathol.; 209: 287?297.
  5. Chen, Z. (2009): The cancer stem cell concept in progression of head and neck cancer. J. Oncol., 2009:894064.
  6. Chiou, SH; Yu, CC; Huang, CY; Lin, SC; Liu, CJ; Tsai, TH et al (2008): Positive cor-relations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma. Clin Cancer Res., 14(13):4085?95.
  7. Choi, S. and Myers, J. (2008): Molecular pathogenesis of oral squmous cell carcinoma: Implications for therapy. J. Dent. Res., 87(1):14-32.
  8. Collins, A., Berry, P., Hyde, C., Stower, M. and Maitland, N. (2005): Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res., 65:10946-10951.
  9. Dalerba, P., Dylla, S., Park, I., Liu, R., Wang, X., Cho, R., et al. (2007): Phenotypic characterization of human colorectal cancer stem cells. Proc. Natl. Acad. Sci. U S A, 104:10158-10163.
  10. Forghanifard MM, Khales SA, Javdani-Mallak A, Rad A, Farshchian M, Abbaszadegan MR (2014): Stemness state regulators SALL4 and SOX2 are involved in progression and invasiveness of esophageal squamous cell carcinoma. Medical Oncology ; 31(4):1-8.
  11. Forghanifard, MM; Taleb, S; Abbaszadegan, MR (2015): Notch Signaling Target Genes are Directly Correlated to Esophageal Squamous Cell Carcinoma Tumorigenesis. Pathology & Oncology Research; 21(2):463-7.
  12. Gong, Y., Yarrow, P., Carmalt, H., Kwun, S., Kennedy, C., Lin, B. et al. (2007): Overexpression of Cripto and its prognostic significance in breast cancer: A study with long-term survival. EJSO, 33(4):438-443.
  13. Hassabou, N; Kasem, R and Farag, H (2011): Precancerous and Cancerous Stem Cells in Oral Ep ithelial Dysplasia and Oral Squamous Cell Carcinoma (An Immunofluorescent Study). E.D.J. Vol. 57, No. 3
  14. Hema,KN; ?Rao, K; ?Devi, U; ?Priya, NS; Smitha, T and Sheethal, HS (2014): Immunohistochemical study of CD44s expression in oral squamous cell carcinoma-its correlation with prognostic parameters. J Oral Maxillofac Pathol. May-Aug; 18(2): 162?168
  15. Hermann, P., Huber, S., Herrler, T., Aicher, A., Ellwart, J., Guba, M., et al., (2007): Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell Stem Cell, 1:313-323.
  16. Jemal, A., Sieger, R., Ward, E., Murray, T., Xu, J., Smigal, C. and Thun, M. (2006): Cancer statistics, 2006. CA. Cancer J. Clin., 56(2):106-130.
  17. Klauzinska, M., Castro, N., Rangel, M., Spike, B., Gray, P., Bertolette, D. et al. (2014): The multifaceted role of the embryonic gene Cripto-1 in cancer, stem cells and epithelial-mesenchymal transition. Semin. Cancer Biol., 29:51-58.
  18. Klonisch, T; Wiechec, E; Hombach-Klonisch S., Ande S., Wesselborg S., Schulze-Osthoff K., Los M (2008): Cancer stem cell markers in common cancers ? therapeutic implications.Trends Mol. Med.; 14:450-460.
  19. Lee, C.Jl Dosch J., Simeone D.M (2008): Pancreatic cancer stem cells. Journal of Clinical Oncology; 26: 2806-2812.
  20. Liu, Q; Cui, X; Yu, Xi; Bian, B.S.J; Qian, F; Hu, Xu.G; Ji, C; Yang, L; Ren, Y; Cui, W; Zhang, X; Zhang, P; Wang, J; Cui, Y.H and Bian, Xiu (2017): Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma. Molecular Cancer, 16:81,1-12.
  21. Mahmoudian, R. A; Abbaszadegan, M.R; Forghanifard, M.M; Moghbeli, M; Moghbeli, F; Chamani, J and Mehran Gholamin, M (2017): Biological and Clinicopathological Significance of Cripto-1 Expression in the Progression of Human ESCC. Reports of Biochemistry & Molecular Biology Vol.5, No.2:1-8.
  22. Mariotto, A., Yabroff, K., Shao, Y., Feuer, E. and Brown, M. (2011): Projections of the cost of cancer care in the United States: 2010-2020. J. Nati. Cancer Inst., 103(2):117-128.
  23. Normanno, N., De Luca, A., Maiello, M., Bianco, C., Mancino, M., Strizzi, L., et al (2004). CRIPTO-1: a novel target for therapeutic intervention in human carcinoma. Int J Oncol, 25, 1013-1020.
  24. Okamoto, A., Chikamatsu, K., Sakakura, K., Hatsushika, K., Takahashi, G., Masuyama, K. (2009): Expansion and characterization of cancer stem-like cells in squamous cell carcinoma of the head and neck. Oral Oncol., 45(7):633-639.
  25. Oshimori, N., Oristian, D. and Fuchs, E. (2015): TGF-beta promotes heterogeneity and drug resistance in squamous cell carcinoma. Cell, 160(5):963-976.
  26. Perez, A; Neskey, DM; Wen, J; Pereira, L; Reategui, EP; Goodwin, WJ; et al (2013): CD44 interacts with EGFR and promotes head and neck squamous cell carcinoma initiation and progression. Oral Oncol., 49(4):306?13.
  27. Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P. et al (2007). Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc.Natl.Acad.Sci. USA, 104: 973-978.
  28. Satpute, P., Hazarey, V., Ahmed, R. and Yadav, L. (2013): Cancer stem cells in head and neck squamous cell carcinoma: A review. Asian Pac. J. Cancer Prev., 14 5579-5587.
  29. Schier, AF (2003): Nodal signaling in vertebrate development. Annual review of cell and developmental biology; 19(1):589-621.
  30. Strizzi, L; Bianco, C; Normanno, N; Seno, M; Wechselberger, C; Wallace‐Jones, B et al (2004): Epithelial mesenchymal transition is a characteristic of hyperplasias and tumors in mammary gland from MMTV‐Cripto‐1 transgenic mice. Journal of cellular physiology; 201(2):266-76
  31. Tirino, V., Desiderio, V., d'Aquino, R., De Francesco, F., Pirozzi, G., Graziano, A., et al. (2008): Detection and characterization of CD133+ cancer stem cells in human solid tumours. PLoS One, 3(10):e3469.
  32. Tsai, LL; Hu, FW; Lee, SS; Yu, CH; Yu, CC; Chang, YC (2014): Oct4 mediates tumor initiating properties in oral squamous cell carcinomas through the regula-tion of epithelial-mesenchymal transition. PLoS One, 9(1):e87207.
  33. Tysnes, B., Satran, H., Mork, S., Margaryan, N., Eide, G., Petersen K., et al. (2013): Age-Dependent Association between Protein Expression of the Embryonic Stem Cell Marker Cripto-1 and Survival of Glioblastoma Patients. Transl. Oncol., 6:732-741.
  34. Wei, B., Jin, W., Ruan, J., Xu, Z., Zhou, Y., Liang, J. et al. (2015): Cripto-1 expression and its prognostic value in human bladder cancer patients. Tumor Biology, 36(2):1105-1113.
  35. Wei, B; Jin, W; Ruan, J; Xu, Z; Zhou, Y; Liang, J et al (2015). Cripto-1 expression and its prognostic value in human bladder cancer patients. Tumor Biology; 36(2):1105-13.
  36. Wu, D., Shi, Z., Xu, H., Chen, R., Xue, S. and Sun, X. (2017): Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells. J. Biosci., 42(3):405-416.
  37. Wu, Z., Li, G., Wu, L., Weng, D., Li, X. and Yao, K. (2009): Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma. BMC cancer, 9(1):315.
  38. Yanamoto S, Kawasaki G, Yamada S, Yoshitomi I, Kawano T, Yonezawa H, et al. (2011): Isolation and characterization of cancer stem-like side population cells in human oral cancer cells. Oral Oncol., 47(9):855-60.
  39. Yanamoto, S., Kawasaki, G., Yamada, S., Yoshitomi, I., Kawano, T., Yonezawa, H., et al. (2011): Isolation and characterization of cancer stem-like side population cells in human oral cancer cells. Oral Oncol. 47(9):855-860.
  40. Ye, X., Tam, W., Shibue, T., Kaygusuz, Y., Reinhardt, F., Ng Eaton, E. and Weinberg, R. (2015): Distinct EMT programs control normal mammary stem cells and tumour-initiating cells. Nature, 525(7568):256-260.
  41. Yoon H-J, Hong J-S, Shin W-J, Lee Y-J, Hong K-O, Lee J-I et al. (2011): The role of Cripto-1 in the tumorigenesis and progression of oral squamous cell carcinoma. Oral oncology.;47(11):1023-31.
  42. Yoon, H., Hong, J., Shin, W., Lee, Y., Hong, K., Lee, J., et al., (2011): The role of Cripto-1 in the tumorigenesis and progression of oral squamous cell carcinoma. Oral Oncol., 47(11):1023-1031.
  43. Yu, YH; Morales, J; Feng, L; Lee, JJ; El-Naggar, AK; Vigneswaran, N (2015): CD147 and Ki-67 overexpression confers poor prognosis in squamous cell carcinoma of oral tongue: a tissue microarray study. Oral Surg Oral Med Oral Pathol Oral Radiol., 119(5):553?65.
  44. Zhang, J-G., Zhao, J. and Xin, Y. (2010): Significance and relationship between Cripto-1 and p-STAT3 expression in gastric cancer and precancerous lesions. World J. Gastroenterol., 16(5):571-577.
  45. Zhang, Q., Shi, S., Yen, Y., Brown, J., Ta, J. and Le, A. (2010): A subpopulation of CD133(+) cancer stem-like cells characterized in human oral squamous cell carcinoma confer resistance to chemotherapy. Cancer Lett., 289:151-160.
  46. Zini, A., Czerninski, R., Harold, D. and Cohen, S. (2010): Oral cancer over four decades: Epidemiology, trends, histology and survival by anatomical sites. J. Oral Pathol. Med., 39(4):299-305.

[Yasmine Alaa El-Din, Amina Fouad Farag and Nadia Fathy Hassabou. (2018); IDENTIFICATION OF CANCER STEM CELLS IN ORAL SQUAMOUS CELL CARCINOMA USING CRIPTO-1. Int. J. of Adv. Res. 6 (Jan). 411-419] (ISSN 2320-5407). www.journalijar.com

YASMINE Alaa El-Din

DOI:

Article DOI: 10.21474/IJAR01/6235      
DOI URL: https://dx.doi.org/10.21474/IJAR01/6235

Download Full Paper

Download PDF No. of Downloads: 15 | No. of Views: 90