SCREENING AND EXTRACTION OF BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED SOILS.
- Department of Microbiology, Mohamed Satak College Of Arts & Science, Chennai, Tamil Nadu.
323 Downloads
724 Views
Abstract
Biosurfactants produced by bacteria are surface active compounds involved in the degradation of hydrocarbons. They are heterogeneous group of surface active molecules produced by microorganisms, which adhere to the cell surface or are excreted extra cellularly in the growth medium. The biosurfactants producing microbes are helpful in bioremediation of heavy metals, pesticides and hydrocarbon contaminated sites. They are also used as bio control agent to protect plant against various diseases, resulting in higher crop yields. The present study was aimed to isolate potential biosurfactants producing bacteria from five different oil contaminated sites and tested for biosurfactant production using different techniques includes oil spreading test, emulsification test, haemolysis test and drop collapse assay.
Keywords
Article Analytics
References
- Abu-Ruwaida AS, Banat IM, Haditirtos Salem A, Kadri M. Isolation of biosurfactant producing bacterial product characterization and evaluation Acta Biotechnology1991; 11:315-324.
- Aparna A, Srinikethan G & Hedge S, Effect of addition of biosurfactant produced by Pseudomonas aeruginosa on biodegradation of crude oil,6,2011, 71-75.
- Atlas RM, Philip J, Bioremediation: applied microbial solutions for real-world environmental cleanup. American society for microbiology (ASM)Press 2005; 78-105.
- Bodour AA, Drees KP and Maier RM (2003). Distribution of biosurfactant producing bacteria in undisturbed and contaminated arid south-western soil. Appln. Environment. Microbiol., 69:3280-3287.
- Cameotra SS, Makkar RS (1998). Synthesis of biosurfactants in extreme conditions. Appln. microbiol. Biotechnol. 50:520-529.
- Chayabutra C, Wu, J, and Ju,L, 2001. ?Rhamnolipid production by Pseudomonas aeruginosa under denitrification effect of limiting nutrients and carbon substrates?. Biotechnology bioengineering, vol . 72, pp.25-33.
- Chikere CB.,Okpokwasili GC., Chikere BO. Monitering of microbial hydrocarbon remediation in soil. Biotechnology 2011: 1 : 117-138.
- Chirwa EMN, Bezza FA. Petroleum hydrocarbons spill in the environment and abundance of microbial community capable of biosurfactant production. J. Petroleum environment biotechnology. 2015; 6:237.
- Christofi N and Irshina I, 2002? Microbial surfactants and their use in field studies of soil remediation ?J. appl, Microbiol. Vol. 93, pp. 915-929.
- Das K, and Mukherjee AK (2005). Characterization of biochemical properties and biological activities of biosurfactant produced by P.aeruginosa mucoid and non-mucoid strains isolated from hydrocarbons contaminated soil samples. Appln.Microbiol. Biotechnol.,69: 192-199.
- Das N & Chandran P, Characterization and sphorolipid biosurfactant produced by yeast sps grown on diesel oil, Indian Journal of science and nature, 2(1), 2011, 63-71.
- Desai JD & Banat IM, Microbial production of biosurfactant and their commercial potentials, Microbiology and molecular biology review,61, 1997, 71-75.
- Desai JD, Banat IM (1997). Microbial production of biosurfactant and their commercial potential. Microbial molecular biology. Rev. 61(1): 47-64.
- Fitcher A (1992). Biosurfactant moving towards industrial application. Tibtech 10:208-217.
- Jain D, Collins-Thompson D & Lee H., 1991.? A drop collapsing test for screening surfactants producing microorganisms. J.Microbial methods, vol.13,pp.271-279.
- Kosari N (2001). Biosurfactants and their applications for soil bioremediation. Food technology. Biotechnology. 39: 295-304.
- Mulligan C, Cooper D, and Neufield R., (1994) ?Selection of microbes producing biosurfactants in media without hydrocarbons.? Fermentation technology, Vol.62, pp:311-314.
- Plaza G, Ziawiony I, Banat I M., 2006. Use the different methods for the detection of thermophilic biosurfactant producing bacteria from hydrocarbon contaminated and bioremediated soils.J. Petrol.sci.eng, vol.50, pp 71-77.
- Rahman K, Rahman T, Korkoutas Y, Petsas I, Marchant R and Banat I., 2003.? Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and macronutrients? Bioresources technology, vol 90, pp. 159-168.
- Rodrigues LR, Teixeri JA, Van Der Mei HC & Oliveira R, Physiochemical and functional characterization of a biosurfactant produced by Lactococcus lactis. Colloids surf B biointerfacec, 49,2006, 79-86.
- Schulz D, Passeri A, Schmidt M.,1991. Marine biosurfactant primary screening biosurfactants among crude oil degrading marine microorganisms from the North Sea. Z. Naturforsch, vol.46, pp. 197-203.
- Soberon-Charaz G, Maier RM (2011). Biosurfactants a general overview. In: Soberon-Charaz G, editor. Biosurfactants. Springer- Verlag. Berlin, Germany. Pp.1-11.
- Van Hamme JD, Singh A, Ward OP. Physiolocial aspects, part 1 in a series of paper devoted to surfactants in microbiology and biotechnology. Biotecnnology advance; 2006; 24: 604-620.
- Whang LM, Liu PWG, Macc, Cheng SS (2008). Application of biosurfactant, rhamnolipid and surfactin, for enhanced biodegradation of diesel contaminated water and soil. J.Hazard Mater. 151:155-163.
- Yateem A,Balba MT, Al-Shayji Y and Al-Awadhi M (2002) Isolation and characterization of biosurfactant producing bacteria from oil contaminated soil. Soil sediment contamination., 11:41-55.
- Youseff NH, Duccan KE, Nagle DP, Sarage KN, Knapp RM and Mclnerney MJ, (2004). Comparison of methods to detect biosurfactants production by diverse microorganisms, J. Microbiol. Mech., 56: 339-347.
- Youssef N, Duncan K, & Nagle D., 2004. Comparison of methods to detect biosurfactants production by diverse microorganisms. J.Microbial methods. Vol.56, pp.339-347.
- Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM& Michael JM. Comparison of methods to detect biosurfactant production by diverse microorganisms, J.Microbial methods,56,2004, 339-347.
- Zajic JE, Stiffens W (1994). Biosurfactants. CRCRev. Biotechnol. 1:87-106.
How to Cite This Article
B. F. Paul Beulah, A. Reena and M. Uma Maheswari. (2018); SCREENING AND EXTRACTION OF BIOSURFACTANT PRODUCING BACTERIA FROM OIL CONTAMINATED SOILS., Int. J. of Adv. Res., 6 (02), 1873-1878, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/6630
Corresponding Author
This work is licensed under a Creative Commons Attribution 4.0 International License.





