14Mar 2018

MOLECULAR IDENTIFICATION OF STREPTOMYCES PHAEOPURPUREUS ISOLATED FROM MARINE SEDIMENTS, VISAKHAPATNAM, USING 16S RRNA GENE SEQUENCE ANALYSIS.

  • Department of Botany, Andhra University, Visakhapatnam, India. Pin- 530 003.
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In order to obtain different actinomycetes isolates from the Visakhapatnam marine soils, an isolation study was carried out by using different growth media. A molecular taxonomic method, 16S rDNA gene sequencing, was applied to obtain high virulent isolate (DHB-603) isolated from Visakhapatnam marine soils. Phylogenetic dendrograms based on 16S rDNA nucleotide sequences were constructed by using neighbour-joining algorithm and maximum likelihood methods. In the light of phylogenetic analyses, it was determined that DHB-603 showed 99.5% similarity with Streptomyces phaeopurpureus, and in phylogenetic tree also DHB-603 and Streptomyces phaeopurpureus were in the same branch. Soil isolate obtained from this study contribute to taxonomy of Streptomyces and also many species will emerge.

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[Sari Dharmaveera, Sandhya Deepika D and Yamuna M M. (2018); MOLECULAR IDENTIFICATION OF STREPTOMYCES PHAEOPURPUREUS ISOLATED FROM MARINE SEDIMENTS, VISAKHAPATNAM, USING 16S RRNA GENE SEQUENCE ANALYSIS. Int. J. of Adv. Res. 6 (Mar). 914-920] (ISSN 2320-5407). www.journalijar.com

Sari Dharmaveera
Department of Botany, Andhra University, Visakhapatnam, India.

DOI:

Article DOI: 10.21474/IJAR01/6748      
DOI URL: https://dx.doi.org/10.21474/IJAR01/6748

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