ISOLATION, SCREENING AND IDENTIFICATION OF BIOSURFACTANT PRODUCING FUNGAL STRAINS.

  • A. M. Reddy Memorial College of Pharmacy, Petlurivaripalem, Narasaraopet.
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Biosurfactants are the surface active compounds which are of microbial origin that are produced by different strains of bacteria fungi and actinomycetes. Soil samples were collected and 10 fungal colonies were isolated using pour plate method. Out of 10 fungal strains three were selected for further studies based on foaming activity. Preliminary and confirmatory tests were used for the identification of biosurfactant produced by three fungal strains. PS7 showed high biosurfactant production in emulsification index compared to other isolates. In this study morphology of fungal isolates were seen using trinocular microscope. Biosurfactants had different applications in the areas of oil recovery, cosmetics, agriculture, food processing.


  1. Cooper, D. G. & Zajic, J. F1980 Surface acting compounds from microorganisms. Advances in Applied Microbiology 26, 229-253
  2. Ellaiah P, Prabakar T, Sreekanth M, Taleb A T, Bhima P&Saisha V, Production of Glycolipids containing biosurfactant by Pseudomonas species, Indian? J ExpBiol, 40(2002) 1083-1086.
  3. GarimaBhardwaj, Swaranjit Singh Cameotra and Harish Kumar ChopraBiosurfactants from Fungi: A Review Volume 4 ? Issue 6 ? 1000160J Pet Environ Biotechnol ISSN: 2157-7463 JPEB 2013.s
  4. Ghurye, G. L Vipulanandan, C& Willson, R. C. 1994 A practical approach to biosurfactant production using non aseptic fermentation of mixed cultures. Biotechnology and Bioengineering 44, 661-666
  5. seghalKiran, T.A. Hema, R. Gandhimathi, Joseph Selvin, T. Anto Thomas, T. Rajeetha, K. Natarajaseenivasan , Optimization and production of biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3, Colloids and surface B:Biointerfaces 73(2009) 250-256.
  6. Kalyani A.L.T, Naga Sireesha G, Aditya A.K.G, GirijaSankar G, Prabhakar T ,Production Optimization of Rhamnolipid Biosurfactant by Streptomyces coelicoflavus (NBRC 15399T) using Plackett-Burman design, ISSN 2321-9122 EJBB 2014; 1 (5): 07-13,2014.
  7. Kamal preet kaur, Seema sangwan, Harpreet kaur, Biosurfactant production by yeast isolated from hydrocarbon polluted environment, Environ Monit Assess (2017) 189:603
  8. Karanth, N.G.K., P.G. Deo and N.K. Veenanadig, 1999. Microbial production of biosurfactants and their importance. Curr. Sci., 77: 116-126.
  9. Morikawa M, Hirata Y and Imanaka T, A study on the structure ?function relationship of lipopeptide biosurfactants, BiochemBiophyActa, 1488(2000) 211-218.
  10. G. Carrillo , C. Mardarag, S. I. Pitta-Alvarez and A. M. Giulietti Isolation and selection of biosurfactant producing bacteria, World journal of microbiology and biotechnology , vol. 12, 1996
  11. Samer M. Al-Hulu Babylon university, college of science, Isolation and identification of actinomycetes with biosurfactant activity vol.(3) No.(2), 2016
  12. Youssef NH, Duncan KE, Nagle DD, Savage KH, Knapp RM, McInerney MJ. Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods 2004; 56:339347.

[K. Revathi Sushma, G. Sai Kiranmai, D. Nandini, G. Gowtham, L. Mallikarjunarao and Dr. A. M. S Sudhakar Babu. (2018); ISOLATION, SCREENING AND IDENTIFICATION OF BIOSURFACTANT PRODUCING FUNGAL STRAINS. Int. J. of Adv. Res. 6 (Apr). 1036-1041] (ISSN 2320-5407). www.journalijar.com


KATAKAM REVATHI SUSHMA
A.M.REDDY MEMORIAL COLLEGE OF PHARMACY

DOI:


Article DOI: 10.21474/IJAR01/6947      
DOI URL: https://dx.doi.org/10.21474/IJAR01/6947