Vol. 6 (04) pp. 1411-1416 DOI: 10.21474/IJAR01/7002

IMMUNOPATHOGENESIS OF ENTAMOEBA HISTOLYTICA AT NEWLY DIAGNOSED MALE PATIENTS, IN THE EASTERN REGION OF IRAQ.

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  • Department of Clinical Laboratory Sciences, College of Pharmacy, AL-Mustansiriyah University. Baghdad, Iraq.
  • Kamal Al-Samarraee Teaching Hospital for Infertility and IVF.
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Abstract

This study aims to investigate the effect of Entamoeba histolytica infection on the cellular immunity of the patients, through measuring the following immune markers: IL-1α, IL-2 and IL-8 during acute amoebiasis, and to find out the correlation of serological parameter with the severity of infection. The study included 110 individuals (male only), 55 patients were diagnosed with Entamoeba histolytica, and 55 cases as a healthy control group. Immune markers IL-1α, IL-2 and IL-8 were assessed throughout the course of the infection. Results showed elevated levels of IL-1α and IL-2 in patients group compared with the healthy control group (8.216? 1.656) and (4.263?1.128) respectively (P <0.001). In addition, results showed a significant increase in IL-8 (P <0.001) with a confidence interval of (3.512-4.881).

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How to Cite This Article

Zaid Habeeb Abd-Alkareem, Jaber Hameed hussien, Qays Ahmed Al-Kafaji and Mohammed Al-Aaraji. (2018); IMMUNOPATHOGENESIS OF ENTAMOEBA HISTOLYTICA AT NEWLY DIAGNOSED MALE PATIENTS, IN THE EASTERN REGION OF IRAQ., International Journal of Advanced Research (IJAR), 6 (04), 1411-1416, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/7002

Corresponding Author

Zaid Habeeb Abd-Alkareem
Clinical pharmacists

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References

  1. Abbas, Z., Ahmad, A. & Khan, A.H. (1997) Syndrome of inappropriate secretion of antidiuretic hormone (SIADH) in amoebic liver abscess. J. Infect. 34: 79-81.
  2. Ahn CS, Kim JG, Shin MH, Lee YA, Kong Y Comparison of Secretome Profile of Pathogenic and Non-Pathogenic Entamoeba histolytica. Proteomics. 2018 Feb 6.
  3. Al-Oumashi GD. Evaluation of immunoblobulin M(IL-1) assay true infection of E. histolytica. Iraqi Journal of Biotechnology. 2012 Vol(11): 2:445-454.
  4. Bercu TE, Petri WA, Behm JW. Amebic colitis: new insights into pathogenesis and treatment. Curr Gastroenterol Rep. 2007; 9:429.
  5. Begum, S., Quach, J., & Chadee, K. (2015). Immune Evasion Mechanisms of?Entamoeba histolytica: Progression to Disease.?Frontiers in Microbiology,?6, 1394.
  6. Bickel M. The role of interleukin-8 in inflammation and mechanisms of regulation. J Periodontol. 1993 May;64(5 Suppl):456-60.
  7. Bruchhaus I., Loftus B.J., Hall N., Tannich E.?The Intestinal Protozoan Parasite Entamoeba histolytica Contains 20 Cysteine Protease Genes, of Which Only a Small Subset Is Expressed during In Vitro Cultivation?.?Eukaryotic Cell?2003. 2 (3): 501-509.
  8. Carrero JC1, Cervantes-Rebolledo C, Aguilar-D?az H, D?az-Gallardo MY, Laclette JP, Morales-Montor J. The role of the secretory immune response in the infection by Entamoeba histolytica. Parasite Immunol. 2007 Jul;29(7):331-8.
  9. Capobianco MP, Cassiano, GC, da Cruz Furini AA, Storti de Melo LM, Domingos CRB, et al. Human Interleukin 2 (IL-2) Promotion of Immune Regulation and Clinical Outcomes: A Review. J Cytokine Biol. 2016. 1:109.
  10. Campbell D1, Gaucher D, Chadee K. Serum from Entamoeba histolytica-infected gerbils selectively suppresses T cell proliferation by inhibiting interleukin-2 production. J Infect Dis. 1999 Jun;179(6):1495-501.
  11. Choi HS1, Kim JW, Cha YN, Kim C(2006) A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells<J Immunoassay Immunochem. 2006;27(1):31-44.
  12. Diaz-Valencia JD, P?rez-Y?pez EA, Ayala-Sumuano JT, Franco E, Meza I. A surface membrane protein of Entamoeba histolytica functions as a receptor for human chemokine IL-8: its role in the attraction of trophozoites to inflammation sites. Int J Parasitol. 2015 Dec;45(14):915-23.
  13. Dey I, Chadee K. Prostaglandin E2 Produced by Entamoeba histolytica Binds to EP4 Receptors and Stimulates Interleukin-8 Production in Human Colonic Cells. Infection and Immunity. 2008;76(11):5158-5163.
  14. Eckmann L, Reed SL, Smith JR, Kagnoff MF. Entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin-1 alpha. J Clin Invest. 1995 Sep;96(3):1269-79.
  15. Espinosa-Cantellano M and Mart?nez-Palomo A. Pathogenesis of Intestinal Amebiasis: From Molecules to Disease. Clin Microbiol Rev. 2000 Apr; 13(2): 318?331.
  16. Garcia-zepeda, Rojas-lopez MA, Esquivel-velazquez, Osto S. Regulation of the inflammatory immune response by the cytokine/chemokine network in amoebiasis. 2007?;29(12): 679?684.
  17. Gaffen SL, Liu KD. "Overview of interleukin-2 function, production and clinical applications". Cytokine. 2004. 28 (3): 109?23.
  18. Khan NT and Jahan. Prevalence of E. histolytica Associated Dysentery in Children in Satellite Town, Quetta. Epidemiology (Sunnyvale). 2017, 7:1,DOI: 10.4172/2161-1165.1000290.
  19. Peterson KM, Singh U, Petri WA Jr. Enteric Amebiasis. In: Tropical Infectious Diseases: Principles, Pathogens and Practice, 3rd ed, Guerrant R, Walker DH, Weller PF (Eds), Saunders Elsevier, Philadelphia. 2011. p.614.
  20. Raeburn CD, Sheppard F, Barsness KA, Arya J, Harken AH (2002) Cytokines for surgeons. Am J Surg183:268-273.
  21. Skappak C, Akierman S, Belga S, et al. Invasive amoebiasis: A review of Entamoeba infections highlighted with case reports. Canadian Journal of Gastroenterology & Hepatology. 2014;28(7):355-359.
  22. Sharma M, Vohra H, Bhasin D. Enhanced pro-inflammatory chemokine/cytokine response triggered by pathogenic Entamoeba histolytica : basis of invasive disease. Parasitology. 2005 Dec;131(Pt 6):783-96.
  23. Yu Y and Chadee K. Entamoeba histolytica Stimulates Interleukin-8 From Human Colonic Epithelial Cells Without Parasite-Enterocyte Contact Institute of Para ref.
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