Vol. 6 (04) pp. 1089-1093 DOI: 10.21474/IJAR01/6955

DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN SPUTUM SAMPLES WITH MPB 64 GENE PRIMERS USING POLYMERASE CHAIN REACTION.

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  • Assistant Professor, Noida International University, Greater Noida.
  • Professor, Santosh Medical College, Ghaziabad.
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Abstract

Tuberculosis (TB) is preventable and curable, but it can lead to death if no actions are taken. In order to prevent transmission, it is necessary to identify infectious TB patients in a timely manner. The present study was aimed to detect the mycobacterium tuberculosis DNA in sputum samples with MPB 64 gene primers using PCR. Hundred patients were enrolled in the study based on inclusion and exclusion criteria. Sputum samples were collected and processed for the required procedure. Ziehl-Neelsen staining was done and slides were examined under microscope. DNA was extracted from the samples and amplified using PCR. Staining results of both spot and morning samples was compared under microscope with grading.

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How to Cite This Article

Ajab Singh Choudhary, Dakshina Bisht Jyoti Sharma and Jyoti Choudhary. (2018); DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN SPUTUM SAMPLES WITH MPB 64 GENE PRIMERS USING POLYMERASE CHAIN REACTION., Int. J. of Adv. Res., 6 (04), 1089-1093, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/6955

Corresponding Author

Ajab Singh Choudhary
Assistant Professor, Noida International University, Greater Noida
Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.